Can component‐based microarray replace fluorescent enzimoimmunoassay in the diagnosis of grass and cypress pollen allergy?

Cabrera‐Freitag, Paula; Goikoetxea, M. J.; Beorlegui, Carmen; Gamboa, P. M.; Gastaminza, Gabriel; Fernández-Benítez, M.; Ferrer, Marta; Blanca, Miguel; Sánz, M L

doi:10.1111/j.1365-2222.2011.03818.x

Cited by 31 publications

(20 citation statements)

References 16 publications

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“…For example, ImmunoCAP and ISAC tests have a similar sensitivity and specificity in patients diagnosed with grass and cypress pollen allergy on the basis of clinical history and SPTs [11]. SPT and ImmunoCAP tests are generally comparable in predicting or defining symptomatic food hypersensitivity [12].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Performance of Skin Prick, ImmunoCAP, and ISAC Tests in the Diagnosis of Patients with Allergy

Griffiths

El‐Shanawany

Jolles

et al. 2017

Int Arch Allergy Immunol

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Background: Allergy is diagnosed from typical symptoms, and tests are performed to incriminate the suspected precipitant. Skin prick tests (SPTs) are commonly performed, inexpensive, and give immediate results. Laboratory tests (ImmunoCAP) for serum allergen-specific IgE antibodies are usually performed more selectively. The immuno-solid phase allergen chip (ISAC) enables testing for specific IgE against multiple allergen components in a multiplex assay. Methods: We retrospectively analysed clinic letters, case notes, and laboratory results of 118 patients attending the National Adult Allergy Service at the University Hospital of Wales who presented diagnostic difficulty, to evaluate which testing strategy (SPT, ImmunoCAP, or ISAC) was the most appropriate to use to confirm the diagnosis in these complex patients, evaluated in a “real-life” clinical service setting. Results: In patients with nut allergy, the detection rates of SPTs (56%) and ISAC (65%) were lower than those of ImmunoCAP (71%). ISAC had a higher detection rate (88%) than ImmunoCAP (69%) or SPT (33%) in the diagnosis of oral allergy syndrome. ImmunoCAP test results identified all 9 patients with anaphylaxis due to wheat allergy (100%), whereas ISAC was positive in only 6 of these 9 (67%). Conclusions: In this difficult diagnostic group, the ImmunoCAP test should be the preferred single test for possible allergy to nuts, wheat, other specific foods, and anaphylaxis of any cause. In these conditions, SPT and ISAC tests give comparable results. The most useful single test for oral allergy syndrome is ISAC, and SPT should be the preferred test for latex allergy.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Performance of Skin Prick, ImmunoCAP, and ISAC Tests in the Diagnosis of Patients with Allergy

Griffiths

El‐Shanawany

Jolles

et al. 2017

Int Arch Allergy Immunol

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“…Although not much data on the diagnostic accuracy of the microarray ISAC in pollen allergy is available, we decided to use this approach because of the possibility it provides to analyze a wide spectrum of pollen-derived component sensitizations. Furthermore, some recent studies have shown similar performances for component-based microarray ISAC and whole-allergen CAP system detection, reporting both high sensitivity and specificity for pollen allergens [6,7]. …”

Section: Introductionmentioning

confidence: 99%

“…Results were expressed as ISAC standardized units (ISU). We did not consider the quantity of the detected specific IgE in further evaluations - results greater than or equal to 0.3 ISU/l were taken as positive [6]. …”

Section: Methodsmentioning

confidence: 99%

A Comprehensive Analysis of Middle-European Molecular Sensitization Profiles to Pollen Allergens

Panzner

Vachová

Vítovcová

et al. 2014

Int Arch Allergy Immunol

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Molecular diagnosis of allergy and microarray technology have opened a completely new avenue of insight into sensitization profiles from both the clinical and the epidemiological point of view. We used this innovative tool in the description of sensitization patterns in pollen-sensitized patients in Middle Europe. Immunoglobulin E detection using 112 different allergenic molecules was carried out employing the ImmunoCAP ISAC microarray system. Sera from 826 patients sensitized to at least one pollen-derived molecule were subjected to analysis. The highest observed sensitization rate was 81.0% to grass-specific molecules (the most frequent being Phl p 1; 69.6%). The second most frequent sensitization was 54.8% to Betulaceae-specific molecules (Bet v 1; 54.2%). Together, grasses and Betulaceae components (and their cosensitizations with other components) comprised the vast majority of pollen sensitizations. Unexpectedly frequently observed sensitizations were those to Cupressaceae-specific molecules (14.1%), Oleaceae-specific molecules (10.8%), and the plane tree-derived molecule Pla a 2 (15.5%). The sensitization rates for all other molecules were within the expected range (Art v 1, 13.6%; Pla l 1, 9.6%; Che a 1, 8.4%; Par j 2, 0.9%; Amb a 1, 0.8%, and Sal k 1, 0.5%). Cross-reacting molecule sensitization rates were found to be 12.4% for profilins, 5.0% for polcalcins, and 6.4% for lipid transfer proteins. Molecular diagnosis of allergy gives a more precise and comprehensive insight into pollen sensitization patterns than extract-based testing, allowing a better understanding of the sensitization process and regional differences. The data presented here may help to improve the diagnostic and allergen-specific treatment procedures in the respective region.

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“…Each ISAC chip can test four sera for all 130 allergens using~50 μL serum and the results are expressed in ISAC Standardized Units (ISU). In comparative diagnostic studies, the ISAC chip has shown a good correlation with fluorescent immunoassay (ImmunoCAP) for pollen and latex allergy and~78 % concordance when tested against a wider panel of 15 allergens [23][24][25]. Proof-of-principle for using bead suspension arrays for IgE antibody and total IgE measurement on the Luminex xMAP platform has been demonstrated [26].…”

Section: Molecular Diagnosticsmentioning

confidence: 98%

Molecular Approaches to Allergen Standardization

Chapman

Briza

2012

Curr Allergy Asthma Rep

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Molecular approaches to allergen standardization include the development of purified natural or recombinant allergen standards whose structural and allergenic properties have been validated, in tandem with certified immunoassays for allergen measurement. Purified allergens can be used individually or incorporated into multiple allergen standards. Multicenter international collaborative studies are required to validate candidate allergen standards and immunoassays, as a prelude to being approved by regulatory agencies. Mass spectrometry is a sophisticated and powerful proteomics tool that is being developed for allergen analysis. Recent results using pollen allergens show that mass spectrometry can identify and measure specific allergens in a complex mixture and can provide precise information of the variability of natural allergen extracts. In future, the combined use of immunoassays and mass spectrometry will provide complete standardization of allergenic products. Molecular standardization will form the basis of new allergy diagnostics and therapeutics, as well as assessment of environmental exposure, and will improve the quality of treatment options for allergic patients.

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Can component‐based microarray replace fluorescent enzimoimmunoassay in the diagnosis of grass and cypress pollen allergy?

Abstract: Component-based microarray ISAC CRD103 and whole-allergen CAP showed high Se and Sp diagnosing equally grass and cypress pollen allergy. The cut-off point for each allergen should be properly applied for both techniques.

Cited by 31 publications

References 16 publications

Comparison of the Performance of Skin Prick, ImmunoCAP, and ISAC Tests in the Diagnosis of Patients with Allergy

Comparison of the Performance of Skin Prick, ImmunoCAP, and ISAC Tests in the Diagnosis of Patients with Allergy

A Comprehensive Analysis of Middle-European Molecular Sensitization Profiles to Pollen Allergens

Molecular Approaches to Allergen Standardization

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