Can co-culturing of two white-rot fungi increase lignin degradation and the production of lignin-degrading enzymes?

Chi, Yujie; Hatakka, Annele; Maijala, Pekka

doi:10.1016/j.ibiod.2006.06.025

Cited by 141 publications

(102 citation statements)

References 31 publications

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“…Several authors have reported increased Lac and MnP activities due to two-speciesinteractions (Baldrian 2004;Freitag and Morrell 1992;Snajdr et al 2011;White and Boddy 1992). Chi et al (2007) found that some combinations of two fungi can accelerate the decay of wood due to increases in MnP production relative to that observed in equivalent cases featuring only a single fungal species. Whether enzymes are actually secreted due to fungus-fungus interactions or for other reasons may depend on the community structure and the state of degradation of the colonized wood.…”

Section: The Role Of Wif In Ecosystem Functions and Processesmentioning

confidence: 99%

Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests

et al. 2015

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Fungi play vital roles in the decomposition of deadwood due to their secretion of various enzymes that break down plant cell-wall complexes. The compositions of woodinhabiting fungal (WIF) communities change over the course of the decomposition process as the remaining mass of wood decreases and both abiotic and biotic conditions of the wood significantly change. It is currently not resolved which substrate-related factors govern these changes in WIF communities and whether such changes influence the deadwood decomposition rate. Here we report a study on fungal richness and community structure in deadwood of Norway spruce and European beech in temperate forest ecosystems using 454 pyrosequencing. Our aims were to disentangle the factors that correspond to WIF community composition and to investigate the links between fungal richness, taxonomically-resolved fungal identity, and microbial-mediated ecosystem functions and processes by analyzing physico-chemical wood properties, lignin-modifying enzyme activities and wood decomposition rates. Unlike fungal richness, we found significant differences in community structure between deadwood of different tree species. The composition of WIF communities was related to the physico-chemical properties of the deadwood substrates. Decomposition rates and the activities of ligninmodifying enzymes were controlled by the succession of the fungal communities and competition scenarios rather than fungal OTU richness. Our results provide further insights into links between fungal community structure and microbialmediated ecosystem functions and processes.

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Section: The Role Of Wif In Ecosystem Functions and Processesmentioning

confidence: 99%

Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests

et al. 2015

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“…Two main pathways are used to gain an advantage over the competitor: development of attack or defense mechanisms and improvements in nutrient uptake (12). For instance, the overproduction of a large number of oxidative enzymes, such as laccases, phenoloxidases, and peroxidases, has been demonstrated in multiple interactions (13)(14)(15)(16). As most of these enzymes are also secreted during oxidative and abiotic stresses, it has been suggested that they are part of a defensive function that contributes to the detoxification of reactive oxygen species (ROS).…”

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confidence: 99%

Differential Gene Expression in Pycnoporus coccineus during Interspecific Mycelial Interactions with Different Competitors

Arfi

Levasseur

Record

2013

Appl Environ Microbiol

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Fungi compete against each other for environmental resources. These interspecific combative interactions encompass a wide range of mechanisms. In this study, we highlight the ability of the white-rot fungus Pycnoporus coccineus to quickly overgrow or replace a wide range of competitor fungi, including the gray-mold fungus Botrytis cinerea and the brown-rot fungus Coniophora puteana. To gain a better understanding of the mechanisms deployed by P. coccineus to compete against other fungi and to assess whether common pathways are used to interact with different competitors, differential gene expression in P. coccineus during cocultivation was assessed by transcriptome sequencing and confirmed by quantitative reverse transcription-PCR analysis of a set of 15 representative genes. Compared with the pure culture, 1,343 transcripts were differentially expressed in the interaction with C. puteana and 4,253 were differentially expressed in the interaction with B. cinerea, but only 197 transcripts were overexpressed in both interactions. Overall, the results suggest that a broad array of functions is necessary for P. coccineus to replace its competitors and that different responses are elicited by the two competitors, although a portion of the mechanism is common to both. However, the functions elicited by the expression of specific transcripts appear to converge toward a limited set of roles, including detoxification of secondary metabolites. Interspecific interactions are key elements in the ecology of fungi, which live in complex communities and often have to compete against each other for a territory and the resources it contains (1, 2). Competitive mycelial interactions usually result in replacement (one of the competitors overruns the other) or deadlock (neither fungus gains any territory). The outcome of these interactions will in turn affect the succession of species, community development, and substratum decay (3-5). This ability to eliminate competitors has also been used as a biotechnological tool, with the development of methods using aggressive, strongly antagonistic species, such as Trichoderma sp., to control plantpathogenic and wood rot fungi (1, 6, 7).When antagonistic relations occur, a broad array of responses can be observed, including changes in mycelial morphology at the interaction front, modification of metabolism, and production and secretion of extracellular enzymes (1,(8)(9)(10)(11). Two main pathways are used to gain an advantage over the competitor: development of attack or defense mechanisms and improvements in nutrient uptake (12). For instance, the overproduction of a large number of oxidative enzymes, such as laccases, phenoloxidases, and peroxidases, has been demonstrated in multiple interactions (13-16). As most of these enzymes are also secreted during oxidative and abiotic stresses, it has been suggested that they are part of a defensive function that contributes to the detoxification of reactive oxygen species (ROS). Laccases and peroxidases are also thought to be involved in morpholo...

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“…secrete dominantly CMCase of them in peach and cherry cultures of SSF. 31 The maximum Lac activities in P.eryngii enzyme supernatant ranged from 337.29 ± 11.2 to 2193.06 ± 50.4 U L -1 for peach and cherry wastes with and without pretreatment conditions during 30 days of cultivation as shown in Figure 3. The maximum Lac activities in control conditions of both cultures were attained, and also they were decreased slightly under all pretreatment conditions.…”

Section: Resultsmentioning

confidence: 96%

Peach and Cherry Agroindustrial Wastes: New and Economic Sources for the Production of Lignocellulolytic Enzymes

Akpınar¹,

Ürek

2017

ACSi

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Pleurotus eryngii was incubated on both peach and cherry agroindustrial wastes by solid state fermentation for 30 days without/with pretreatment conditions. The lignocellulosic substrates were pretreated with dilute acid and alkaline solutions, hot water before incubation. The maximum carboxymethyl cellulase and xylanase activities peaked on 3 rd and 5 th days under control conditions of both wastes, respectively. The highest laccase and manganese peroxidase activities reached to their maximum on 17 th day as 2193.06 ± 50.4 UL -1 and 732.73 ± 19.8 UL -1 , respectively. The highest aryl alcohol oxidase activity was obtained as 239.25 ± 7.3 UL -1 in control condition of peach cultures. The used pretreatment methods had generally negative effects on lignocellulolytic enzyme production. The highest lignocellulolytic activities were detected using peach wastes. To results, these wastes could be used as alternative, new and economic energy sources to produce high amounts of lignocellulolytic enzymes.

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Can co-culturing of two white-rot fungi increase lignin degradation and the production of lignin-degrading enzymes?

Cited by 141 publications

References 31 publications

Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests

Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests

Differential Gene Expression in Pycnoporus coccineus during Interspecific Mycelial Interactions with Different Competitors

Peach and Cherry Agroindustrial Wastes: New and Economic Sources for the Production of Lignocellulolytic Enzymes

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