Can chlorination co-select antibiotic-resistance genes?

Lin, Wenfang; Zhang, Menglu; Zhang, Shenghua; Yu, Xiubo

doi:10.1016/j.chemosphere.2016.04.139

Cited by 82 publications

(35 citation statements)

References 37 publications

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“…Additionally, after this stage, a significant level of ARB was detected in the wastewater treatment effluent of SP7. Such observations have also been reported by other studies (8,21,22). The Pearson correlation coefficient also confirmed a significant difference in the ARB content of the SP1 and SP6 wastewater effluents.…”

Section: Discussionsupporting

confidence: 90%

“…The Pearson correlation coefficient also confirmed a significant difference in the ARB content of the SP1 and SP6 wastewater effluents. Lin et al investigated chlorination for disinfection of wastewater and reported its variable effects on the removal/ decrease of ARB and ARGs (21), whereas a study by Munir et al showed that disinfection by chlorination can have an insignificant impact on the ARB reduction (8). On the other hand, Al-Jassim et al demonstrated that the pattern and bacterial diversity alters after disinfection (22).…”

Section: Discussionmentioning

confidence: 99%

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Tracking of chloramphenicol, erythromycin, and sulfamethoxazole antibiotic-resistant bacteria from untreated wastewater effluents to receiving river

Aali

Baragh

Asgari

et al. 2019

Environ Health Eng Manag

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Background: The aim of this study was to investigate the frequency and type of bacteria resistant to chloramphenicol (CHL), erythromycin (E), and sulfamethoxazole (SXT) antibiotics from untreated wastewater effluents to receiving river. Methods: In total, 32 samples were taken from eight sites located in the raw wastewater to the downstream of the receiving Ghotor river in Khoy city. Resistant microorganisms were studied through modified HPC method and CLSI standards. Different and specific colonies were selected and re-cultured in R2A culture medium and enough colonies were used for DNA extraction. Bacterial 16sr RNA target gene was amplified and sequenced. Pseudomonas sp., Comamonas sp., and Thiobacillus sp. were predominant bacterial species identified in water samples. Also, antibiotic-resistant genes (ARGs) including sul1, ermB, and cmlA1 were tracked by PCR. Results: The average total number of antibiotic-resistant bacteria (ARB) increased in the downstream (2.4×102 to 2.6×104 CFU/100 mL). The comparison of the results related to the river’s upstream and downstream also indicated a significant difference between ARB and ARGs contents (P<0.05). The average number of bacteria resistant to SXT, E, and CHL antibiotics was obtained to be 2.3×104 , 2.3×104 , and 3.4×104 CFU/mL, respectively. Finally, the evaluation of water revealed that only the aeration lagoon was able to decline the number of CHL-resistant bacteria (88.9%) while this process increased the number of SXT- (51%) and E- (16%) resistant bacteria. The lowest and highest percentages of the identified genes were related to ermB (12.5%) and sul1 (81.25%), respectively. Conclusion: According to the results, wastewaters play an important role in releasing ARB and their antibiotic resistance genes to downstream of Ghotor rivers in Khoy city.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Tracking of chloramphenicol, erythromycin, and sulfamethoxazole antibiotic-resistant bacteria from untreated wastewater effluents to receiving river

Aali

Baragh

Asgari

et al. 2019

Environ Health Eng Manag

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“…In recent years, the occurrence of ARB and ARGs in drinking water were investigated more and more frequently [5][6][7], as it was revealed that resistance to antibiotics is often correlated with resistance to disinfectants [8,9] (however, it is worth to note that this hypothesis is still controversial, as it was stated by Lin et al [10]) and metals [11], therefore making water treatment plants (WTPs) and tap water distribution systems a niche for ARB dwelling and proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…As the cultivation of bacteria dwelling in tap water may be challenging due to their specific metabolic requirements, sometimes difficult to achieve in laboratory conditions, both ways (culture-dependent and culture-independent) should be implemented in research on ARB, because their usually weak growth may contribute to underestimations of the issue. From the other hand, the selection of ARGs investigated in a study is also a challenge, as antibiotic resistance may be conferred by multiple genes [10]. Hence, the narrowing of tested ARGs group may introduce some biases, alike.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance in tap water during the summer season – preliminary research

Siedlecka

Piekarska

2019

E3S Web Conf.

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Antibiotic resistance of bacteria is regarded as a global health risk and should be monitored in all environments, including tap water distribution systems. In this study, tap water samples were collected from two water treatment plants and selected points-of-use from the water distribution network. The abundances of antibiotic resistant bacteria were determined via culture-dependent method and the presence of selected antibiotic resistance genes was detected via PCR. The influence of the distance of points-of-use from water treatment plants on bacterial loads and antibiotic resistance phenomenon was evaluated. The paper presents preliminary results of a large-scale study concerning spatial and seasonal variation in antibiotic resistance of bacteria dwelling in the tap water system in Wrocław.

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“…The results provide a baseline for the occurrence of resistance genes in the chicken production system without direct selective pressure.Antibiotics 2020, 9, 120 2 of 12 cost-effective and efficient method to collect biomass representing a large population, and this has been used for monitoring the prevalence of pathogens in chicken flocks [8,9]. High-throughput quantitative PCR (HT-qPCR) and deep sequencing approaches have been used in a variety of human, animal and environmental samples to characterize the diversity of antibiotic resistance genes (ARGs) [10][11][12][13][14]. These methods are efficient and effective for the broad-spectrum detection and quantification of ARGs in complex samples.The aim of this study was to assess the presence and diversity of ARGs and mobile genetic elements (MGEs) in poultry farms that had not used antibiotics for 5 years.…”

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confidence: 99%

Antibiotic Resistance Genes in Antibiotic-Free Chicken Farms

et al. 2020

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Rising concern about the use of antibiotics in food production has resulted in many studies on the occurrence of antibiotic resistance genes (ARGs) in animal-associated bacterial communities. There are few baseline data on the abundance of ARGs on farms where chickens are intensively raised with little or no use of antibiotics. This study used a high-throughput quantitative PCR array to survey two antibiotic-free chicken farms for the occurrence of ARGs and mobile genetic elements known to enhance the spread of ARGs. No antibiotics had been used on the study farms for five years prior to this study. The results provide a baseline for the occurrence of resistance genes in the chicken production system without direct selective pressure.Antibiotics 2020, 9, 120 2 of 12 cost-effective and efficient method to collect biomass representing a large population, and this has been used for monitoring the prevalence of pathogens in chicken flocks [8,9]. High-throughput quantitative PCR (HT-qPCR) and deep sequencing approaches have been used in a variety of human, animal and environmental samples to characterize the diversity of antibiotic resistance genes (ARGs) [10][11][12][13][14]. These methods are efficient and effective for the broad-spectrum detection and quantification of ARGs in complex samples.The aim of this study was to assess the presence and diversity of ARGs and mobile genetic elements (MGEs) in poultry farms that had not used antibiotics for 5 years. The WaferGen HT-qPCR system was used to detect and quantify ARGs and MGEs in environmental samples from two chicken farms. The PCR primers targeted ARGs of the major antibiotic classes, and integrases and transposase genes. Caged chicken sheds from two types of farms were selected for investigation; the first was an egg production enterprise which housed caged layer chickens (Farm L), while the second was a broiler breeder farm (Farm B). The results should provide useful baseline information regarding ARG prevalence in the chicken production system without direct selective pressure.

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Can chlorination co-select antibiotic-resistance genes?

Cited by 82 publications

References 37 publications

Tracking of chloramphenicol, erythromycin, and sulfamethoxazole antibiotic-resistant bacteria from untreated wastewater effluents to receiving river

Tracking of chloramphenicol, erythromycin, and sulfamethoxazole antibiotic-resistant bacteria from untreated wastewater effluents to receiving river

Antibiotic resistance in tap water during the summer season – preliminary research

Antibiotic Resistance Genes in Antibiotic-Free Chicken Farms

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