2002
DOI: 10.1053/joca.2002.0528
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Can altered production of interleukin-1β, interleukin-6, transforming growth factor-β and prostaglandin E2 by isolated human subchondral osteoblasts identify two subgroups of osteoarthritic patients

Abstract: These results indicate that IL-6 and PGE(2) production by subchondral Ob can discriminate two subgroups of osteoarthritic patients that cannot otherwise be separated by their expression of cell markers, and that endogenous PGE(2) levels influence IL-6 synthesis in osteoarthritic Ob.

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Cited by 164 publications
(182 citation statements)
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“…Cultures of subchondral bone osteoblasts were prepared as previously described (27). Briefly, bone samples were cut into small pieces and digested for 4 hours with type I collagenase in BGJ b medium (both from Sigma-Aldrich Canada, Oakville, Ontario, Canada), without serum, at a temperature of 37°C in a humidified atmosphere of 5% CO 2 /95% air.…”
Section: Methodsmentioning
confidence: 99%
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“…Cultures of subchondral bone osteoblasts were prepared as previously described (27). Briefly, bone samples were cut into small pieces and digested for 4 hours with type I collagenase in BGJ b medium (both from Sigma-Aldrich Canada, Oakville, Ontario, Canada), without serum, at a temperature of 37°C in a humidified atmosphere of 5% CO 2 /95% air.…”
Section: Methodsmentioning
confidence: 99%
“…Identification of human OA subchondral bone osteoblast subpopulations was performed as previously described (20,27,28). OA osteoblasts producing low levels of PGE 2 (Յ2,000 pg/mg of protein) were classified as L-OA, and those producing high levels of PGE 2 (Ͼ2,000 pg/mg of protein) as H-OA.…”
Section: Methodsmentioning
confidence: 99%
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“…Lesional joint cartilage in the placebo-treated group exhibited morphologic changes, including cartilage fibrillation and fissures, changes in cellularity, such as cloning and hypocellularity, and loss of Safranin O staining, as previously described (11,20,21) (Figure 2). A decrease in the histologic score of the medial femoral condyles, indicative of a reduced severity of the lesions, was observed in dogs with OA treated with pioglitazone (median histologic score 6.25 [range [3][4][5][6][7][8][9][10][11][12] in the placebo group versus 4.25 [range [3][4][5][6][7] in the 15 mg/day pioglitazone group and 5.5 [range [5][6] in the 30 mg/day pioglitazone group); this difference was sig- nificant following a dosage of 15 mg/day pioglitazone as compared with the placebo group (P Ͻ 0.05). This reduction in histologic scores was mainly related to a protective effect of pioglitazone treatment on the severity of structural changes and the loss of Safranin O staining.…”
Section: Characteristics Of the Experimental Animalsmentioning
confidence: 99%
“…The subchondral bone remodeling that takes place during the evolution of OA is believed to be an important factor in cartilage degradation (2)(3)(4). Its contribution seems to be associated with both the abnormal biophysical properties of the tissue and the excess synthesis of many catabolic factors, including growth hormones and cytokines, that can modulate the metabolism of OA cartilage (5)(6)(7)(8).…”
mentioning
confidence: 99%