Campylobacter upsaliensis isolated from a giant hepatic cyst

Ohkoshi, Yasuo; Sato, Toyotaka; Murabayashi, Hiromi; Sakai, Kohei; Takakuwa, Yasunari; Fukushima, Yukari; Nakajima, Chie; Suzuki, Yasuhiko; Yokota, Shinichi

doi:10.1016/j.jiac.2020.02.015

Cited by 5 publications

(5 citation statements)

References 13 publications

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“…The similarity of the concentration calculated for C. jejuni (qPCR) and Campylobacter (CFU enumeration) at day 0 (i.e., on fresh samples), both less represented than Campylobacter concentration at day 0 calculated via qPCR, could reflect the absence in the samples analysed of species such as C. coli, otherwise also prone to thrive on CCDA [29]. However, we could not exclude the presence of some other species, such as C. upsaliensis, whose growth on CCDA has been shown to be involuted [31] but could still be present in the samples, as suggested by the higher concentration of the genus (qPCR) compared to C. jejuni (qPCR).…”

Section: Discussionmentioning

confidence: 90%

“…is standardised [26][27][28], the state in which samples are kept (fresh or frozen) prior to analysis varies between studies and is sometimes not even reported. As bacterial populations, degradation of nucleic acids, proteins and other biological molecules are all affected by the storage temperature [29,31,32] we, therefore, hypothesise that this may affect the bacterial/campylobacter quantification and thus can give us counterfeited results. The current study was thus designed to investigate the effect of storage conditions and storage time on C. jejuni, thus analysed as fresh and stored at -80˚C for up to 62 days.…”

Section: Discussionmentioning

confidence: 99%

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Bacterial concentration and Campylobacter spp. quantification differ when fresh or ultra-frozen samples are analysed over time using molecular biology and culture-based methods

2022

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The study aimed to delineate the robustness of the culture-based and molecular biology methods to assess the total bacterial concentration and Campylobacter jejuni (C. jejuni) quantification in caecal content, analysed as fresh or after being stored immediately at ultralow (-80˚C) temperature at different time points (for 3, 7, 14, 28 and 62 days post collection). The caecal content was collected from birds that were artificially colonised with C. jejuni (invivo), and quantification was performed using both colony-forming unit (CFU) and qPCR. The results showed that storage time affected the output of culture-based analyses but mostly did not alter concentration retrieved via qPCR. After an initial ~4.5 log 10 reduction in CFU observed from fresh (day 0) to frozen samples, bacterial concentration retrieved with culture-based methods seemed to be constant in samples frozen for 3 to 62 days, indicating a possible threshold for C. jejuni loss of viability due to effect of storage temperature. Ranking order analyses, revealed that the molecular biology technique was able to attribute somewhat the same relative C. jejuni concentrations to the samples analysed via qPCR. However, day 0 measurements from culture-based methods were associated with the absence of or negatively weak correlations with the rest of the time points, but ranking order was maintained from day 3 onwards. On the other hand, ranking order correlations were less constant when measuring total bacterial concentration through qPCR. The study suggests that if biological samples can't be analysed as fresh (immediately after collection) and have to be stored prior to analysis, then storage at -80˚C samples be recommended to avoid the temporal-dependent effects on C. jejuni concentrations. In addition, irrespective of the method of analysis, an initial loss of CFU must be factored in when interpreting the results obtained from frozen samples.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Bacterial concentration and Campylobacter spp. quantification differ when fresh or ultra-frozen samples are analysed over time using molecular biology and culture-based methods

2022

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“…Although C. upsaliensis is significantly less pathogenic than C. jejuni, it cannot be considered non-pathogenic (Bojanić et al, 2020). C. upsaliensis has been isolated from human blood, placental tissue, breast abscess, and stool (Couturier et al, 2012), and it was also isolated from an infected large hepatic cyst (Ohkoshi et al, 2020). Moreover, Nakamura et al described a severe fatal infection caused by C. upsaliensis that killed a 70-year-old woman (Nakamura et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, genomic characterization and antimicrobial resistance of Campylobacter spp. isolates in pets in Shenzhen, China

Bi³

et al. 2023

Front. Microbiol.

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The prevalence of Campylobacter spp.in pets is a potential concern for human health. However, little is known about the pet-related Campylobacter spp. in China. A total of 325 fecal samples were collected from dogs, cats, and pet foxes. Campylobacter spp. were isolated by culture, and MALDI-TOF MS was used to identify 110 Campylobacter spp. isolates in total. C. upsaliensis (30.2%, 98/325), C. helveticus (2.5%, 8/325), and C. jejuni (1.2%, 4/325) were the three found species. In dogs and cats, the prevalence of Campylobacter spp. was 35.0% and 30.1%, respectively. A panel of 11 antimicrobials was used to evaluate the antimicrobial susceptibility by the agar dilution method. Among C. upsaliensis isolates, ciprofloxacin had the highest rate of resistance (94.9%), followed by nalidixic acid (77.6%) and streptomycin (60.2%). Multidrug resistance (MDR) was found in 55.1% (54/98) of the C. upsaliensis isolates. Moreover, 100 isolates, including 88 C. upsaliensis, 8 C. helveticus, and 4 C. jejuni, had their whole genomes sequenced. By blasting the sequence against the VFDB database, virulence factors were identified. In total, 100% of C. upsaliensis isolates carried the cadF, porA, pebA, cdtA, cdtB, and cdtC genes. The flaA gene was present in only 13.6% (12/88) of the isolates, while the flaB gene was absent. By analyzing the sequence against the CARD database, we found that 89.8% (79/88) of C. upsaliensis isolates had antibiotic target alteration in the gyrA gene conferring resistance to fluoroquinolone, 36.4% (32/88) had the aminoglycoside resistance gene, and 19.3% (17/88) had the tetracycline resistance gene. The phylogenetic analysis using the K-mer tree method obtained two major clades among the C. upsaliensis isolates. All eight isolates in subclade 1 possessed the gyrA gene mutation, the aminoglycoside and tetracycline resistance genes, and were phenotypically resistant to six classes of antimicrobials. It has been established that pets are a significant source of Campylobacter spp. strains and a reservoir for them. This study is the first to have documented the presence of Campylobacter spp. in pets in Shenzhen, China. In this study, C. upsaliensis of subclade 1 required additional attention due to its broad MDR phenotype and relatively high flaA gene prevalence.

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“…In this study, we conducted a comprehensive comparative genomic analysis of 154 C. upsaliensis strains based on a wide geographic distribution and diverse sample sources from humans, animals, and others. Little studies have described C. upsaliensis isolates so comprehensively until now [ 9 , 14 , 15 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysis

Wang,

Gu,

et al. 2024

Emerging Microbes & Infections

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Campylobacter upsaliensis was the most common Campylobacter species in pets’ gastrointestinal tracts and has been isolated from patients with bacteremia, hemolytic-uremic syndrome, spontaneous abortion, and Guillain-Barré syndrome. However, the genetic characteristics and the full extent of its significance as a human pathogen remain to be fully understood. This study involved an investigation for genomic analysis of 154 strains from different sources and additional antimicrobial resistance profiles of 26 strains for this species. The genomes contained 1,558–1,971 CDS and the genome sizes were estimated to vary from 1.53 Mb to 1.86 Mb, with an average GC content of 34.71%. The entire analyzed genomes could be divided into three clades (A, B, and C) based on ANI and phylogenomic analysis. Significantly, nearly all strains in Clade B were isolated from patient samples, and the virulence-related sequences FlgD, GmhA, and CdtC might serve as determining factors for the classification of Clade B. Half of the tested isolates had MIC values over 64 μg mL −1 for nalidixic acid, gentamicin, and streptomycin. Isolates from pets in China carried more resistant elements in the genomes. This study both provided a comprehensive profile of C. upsaliensis for its genomic features and suggested some pathogenic agents for human infection with this species.

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Campylobacter upsaliensis isolated from a giant hepatic cyst

Cited by 5 publications

References 13 publications

Bacterial concentration and Campylobacter spp. quantification differ when fresh or ultra-frozen samples are analysed over time using molecular biology and culture-based methods

Bacterial concentration and Campylobacter spp. quantification differ when fresh or ultra-frozen samples are analysed over time using molecular biology and culture-based methods

Prevalence, genomic characterization and antimicrobial resistance of Campylobacter spp. isolates in pets in Shenzhen, China

Genetic characteristics and potential pathogenic agents in Campylobacter upsaliensis based on genomic analysis

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