cAMP and adenylate cyclase activity in<i>Streptomyces granaticolor</i>

Dobrová, Z.; Náprstek, J.; Jirešová, M.; Janeček, J.

doi:10.1111/j.1574-6968.1984.tb00725.x

Cited by 11 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Report also showed a simple and an efficient enzymatic synthesis of cAMP from ATP using Bordetella pertussis adenylate cyclase coimmobilized on CNBr-activated agarose beads with its activator, calmodulin (Bellalou, Sarfati, Predeleanu, Ladant, & Bǎrzu, 1988). Additionally, the host cell of Myxococcus xanthus (Yajko & Zusman, 1978) and Streptomyces granaticolor strain ETH7347 (Dobrová, Náprstek, Jireŝová, & Janeĉek, 2010) could also be used to synthesize cAMP. Although these researches successfully used microbiological method to synthesize cAMP based on the microorganism host, still more research work need to be done until the engineered strain could be applied in the industrial food product, or the bioproduct in medical industry.…”

Section: Introductionmentioning

confidence: 99%

Construction of industrial baker’s yeast with high level of cAMP

Dong

Wang

et al. 2019

J Food Biochem

View full text Add to dashboard Cite

Cyclic adenosine monophosphate (cAMP) plays an important role in modulating the activity of microbe cell. In this study, PKA (protein kinase A) activity was weakened through truncation of TPK2 promoter (−150 bp and −300 bp) and gene deletion of BCY1 (encodes the regulatory subunit of PKA), TPK1 and TPK3, generating strains BY9a‐T2‐150 and BY9a‐T2‐300, respectively. High‐performance liquid chromatography analysis showed cAMP levels in BY9a‐T2‐150 and BY9a‐T2‐300 were increased by 5‐ and 18‐fold, respectively, compared with that of parent strain, BY9a. The expression levels of TPK2 gene in two engineered strains were decreased by 95% and 97% compared with that of BY9a, respectively. The PKA activity reflected by heat resistance of engineered strains enhanced compared with parent strain BY9a. This study show a new method to increase the intracellular cAMP concentration in industrial yeast by fine‐tuning of PKA activity, without influence in growth and fermentation properties. Practical applications cAMP as the “second messenger,” is essential for plant, animal, and microorganisms and human life. But its synthesis is still limited by expensive cost and time‐consuming method. We constructed the industrial baker’s yeast with high level of cAMP and desired to be used to produce functional food for relaxing smooth muscle, expanding blood vessels, improving liver function, and promoting nerve regeneration and as a food additive for treating hyperthyreosis and hepatopathy. The methods of two step homologous recombination and backcross operated in this study eliminate the exogenous gene in engineered strains, made it safety to be used in food production. Fine‐tuning of PKA activity in engineered strains ensure produce high level of cAMP and exhibit normal growth performance in engineering strains. Therefore, this work is significant in functional foods product and has the potential to be used in practical application.

show abstract

Section: Introductionmentioning

confidence: 99%

Construction of industrial baker’s yeast with high level of cAMP

Dong

Wang

et al. 2019

J Food Biochem

View full text Add to dashboard Cite

show abstract

Cyclic AMP regulation of tylosin biosynthesis and secondary metabolism in Streptomyces fradiae

Tata

Menawat

1994

Biotech & Bioengineering

View full text Add to dashboard Cite

Adenosine 3':5' cyclic monophosphate seems to regulate antibiotic biosynthesis and secondary metabolism in tylosin-producing cultures of Streptomyces fradiae C373.1. A dose-dependent response is observed by exogenous additions of dibutyryl cyclic AMP (cAMP), and is related to the nutritional status of the culture. Addition of cAMP to cultures growing in nutritionally lean media caused higher cumulative antibiotic tigers and some cellular differentiation compared with the control. In nutritionally rich media, a qualitatively different behavior resulted: an almost instantaneous shift toward secondary metabolism occurred. The response is characterized by extensive cellular differentiation with little growth and only a trace of antibiotic production. The possible role of cyclic AMP n the regulation of tylosin biosynthesis and secondary metabolism and its relation to specific nutrient limitations in synthetic, defined media in Streptomyces fradiae is discussed.

show abstract