Camel milk inhibits inflammatory angiogenesis via downregulation of proangiogenic and proinflammatory cytokines in mice

Abstract

Camel milk has traditionally been used to treat cancer, but this practice awaits scientific scrutiny, in particular its role in tumor angiogenesis, the key step involved in tumor growth and metastasis. We aimed to investigate the effects of camel milk on key components of inflammatory angiogenesis in sponge implant angiogenesis model. Polyester-polyurethane sponges, used as a framework for fibrovascular tissue growth, were implanted in Swiss albino mice and camel milk (25, 50 and 100 mg/kg/day) was administered for 14 days through installed cannula. The implants collected at day 14 post-implantation were processed for the assessment of hemoglobin (Hb), myeloperoxidase (MPO), N-acetylglucosaminidase (NAG), and collagen, which were used as indices for angiogenesis, neutrophil, and macrophage accumulation and extracellular matrix deposition, respectively. Relevant inflammatory, angiogenic, and fibrogenic cytokines were also determined. Camel milk treatment attenuated the main components of the fibrovascular tissue, wet weight, vascularization (Hb content), macrophage recruitment (NAG activity), collagen deposition and the levels of vascular endothelial growth factor (VEGF), interleukin (IL)-1β, IL-6, IL-17, tumor necrosis factor-α, and transforming growth factor-β. A regulatory function of camel milk on multiple parameters of the main components of inflammatory angiogenesis has been revealed, giving insight into the potential therapeutic benefit underlying the anti-cancer actions of camel milk.

Camel milk regulates T‐cell proliferation to alleviate dextran sodium sulphate‐induced colitis in mice

“…The relieving effects of CM on colitis were associated with alleviating oxidative stress by restraining lipid peroxides and restoring glutathione (GSH) in the gut LP. In addition, consuming CM inhibits cell apoptosis as indicated by caspase-3 activity (Alhaider et al, 2014;Uversky et al, 2017). To confirm the role of CM in the prevention of IBD, animal studies are needed.…”

Anticancer Activity of Camel Milk via Induction of Autophagic Death in Human Colorectal and Breast Cancer Cells

“…Camel milk has been reported to possess anti-cancer activity as proved by a few of the in vitro and in vivo studies (Korashy et al, 2012a;Korashy et al, 2012b;Habib et al, 2013;Alhaider et al, 2014;Hasson et al, 2015). Lyophilized camel milk has been shown to inhibit growth and proliferation of many cancer cell types such as human breast cancer BT-474, laryngeal HE-p2 (Hasson et al, 2015), human hepatoma HepG2 (Korashy et al, 2012b) and murine hepatoma Hepa 1c1c7 cells (Korashy et al, 2012a).…”

“…Camel milk is such a dietary food with profound nutraceutical value (Alebie et al, 2017). It forms a high nutritional source with low cholesterol, low sugar, high minerals 3502 (sodium, potassium, iron, copper, zinc and magnesium), high vitamins (vitamin C, B 2 , A and E) and high concentrations of insulin (Haddadin et al, 2008;Alhaider et al, 2014) compared to the ruminant milk.…”

“…A few of the in vitro studies have reported the inhibitory effects of camel milk on cancer cells exerted through the mechanism of apoptosis (Korashy et al, 2012b;Hasson et al, 2015) and antioxidant activities (Habib et al, 2013). Some of the in vivo studies have also shown the potent inhibitory effects of camel milk on pro-inflammatory, pro-angiogenic and pro-fibrogenic cytokines (Alhaider et al, 2014) as well as the ability of camel milk to modulate the expression of known cancer-activating gene and cancer-protective genes at the transcriptional and posttranscriptional levels (Korashy et al, 2012a). However, no studies exist so far, testifying the effect of a commercially available camel milk on cancer cells.…”

Camel Milk with Pegylated Interferon Alfa-2a and Ribavirin for Treatment-Naive Chronic Hepatitis C Genotype 2/ 3: An Open-Label, Randomized Controlled Trial

“…Special bioactives along with camel immune system and its transfer Copyright to milk causes the milk to have pharmacological effects in favor of the liver, including protective effects against viral and bacterial agents, chemical, and toxic substances. Camel milk has potent immunological, anti-allergic (5), antioxidant agent (8), antibacterial (9), anti-diabetic (9), and anti-inflammatory (10), immunomodulatory (5), antiapoptosis (11), and anticancer properties (12). It contains various mineral, vitamins, protective proteins, and essential fatty acids, indicating the potential therapeutic effects underlying the anti-HCV actions.…”