Cambios en el ECG y la actividad enzimática en corazón de rata tras infarto de miocardio: efecto de la TPP y del MnCl2

Tylicki, Adam; Czerniecki, Jan; Godlewska, A.; Kieliszek, Marek; Zebrowski, T; Bielawski, Tomasz; Wójcik, Beata

doi:10.1007/bf03168237

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…Precipitated cellular debris was centrifuged for 15 min at 12 000 g at 4°C, and the clarified supernatants were used for activity measurements. The activity of all TDP-dependent enzymes was determined spectrophotometrically, on the basis of NADH detection at k = 340 nm (Kimmerer, 1987;Gibson et al, 1988;Chamberlain et al, 1996;Tylicki et al, 2008).…”

Section: Extract Preparation and Enzyme Activity Assaysmentioning

confidence: 99%

Thiamine increases the resistance of baker's yeastSaccharomyces cerevisiaeagainst oxidative, osmotic and thermal stress, through mechanisms partly independent of thiamine diphosphate-bound enzymes

et al. 2014

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Numerous recent studies have established a hypothesis that thiamine (vitamin B1 ) is involved in the responses of different organisms against stress, also suggesting that underlying mechanisms are not limited to the universal role of thiamine diphosphate (TDP) in the central cellular metabolism. The current work aimed at characterising the effect of exogenously added thiamine on the response of baker's yeast Saccharomyces cerevisiae to the oxidative (1 mM H2 O2 ), osmotic (1 M sorbitol) and thermal (42 °C) stress. As compared to the yeast culture in thiamine-free medium, in the presence of 1.4 μM external thiamine, (1) the relative mRNA levels of major TDP-dependent enzymes under stress conditions vs. unstressed control (the 'stress/control ratio') were moderately lower, (2) the stress/control ratio was strongly decreased for the transcript levels of several stress markers localised to the cytoplasm, peroxisomes, the cell wall and (with the strongest effect observed) the mitochondria (e.g. Mn-superoxide dismutase), (3) the production of reactive oxygen and nitrogen species under stress conditions was markedly decreased, with the significant alleviation of concomitant protein oxidation. The results obtained suggest the involvement of thiamine in the maintenance of redox balance in yeast cells under oxidative stress conditions, partly independent of the functions of TDP-dependent enzymes.

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Section: Extract Preparation and Enzyme Activity Assaysmentioning

confidence: 99%

Thiamine increases the resistance of baker's yeastSaccharomyces cerevisiaeagainst oxidative, osmotic and thermal stress, through mechanisms partly independent of thiamine diphosphate-bound enzymes

et al. 2014

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“…Notably, previous studies revealed the significant loss of LIFR in failing heart both in humans [ 29 ] and rats [ 30 ]. Also, decreased OGDH activity in rat hearts after myocardial infarction has been observed [ 31 ]. Moreover, hsa-miR-339-5p has been reported to be up-regulated after human left ventricular ischemia [ 32 ] and hsa-miR-134 was an essential regulator in cardiogenesis whose increased expression was strongly associated with increased risk of mortality or heart failure [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Dysregulated long intergenic non-coding RNA modules contribute to heart failure

Pang

Zhang

et al. 2016

Oncotarget

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Long intergenic non-coding RNAs (lincRNAs) are emerging as important regulatory molecules involved in diseases including heart failure. However, little is known about how the lincRNAs work together with protein-coding genes (PCGs) contributing to the pathogenesis of heart failure. In this study, we constructed a comprehensive transcriptome profile of lincRNAs, PCGs and miRNAs using RNA-seq and miRNA-seq data of 16 heart failure patients (HFs) and 8 non-failing individuals (NFs). Through integrating lincRNA and PCG expression profiles, we identified HF-associated lincRNA modules. We identified a heart-specific lincRNA module which was significantly enriched for differentially expressed lincRNAs and PCGs. This module was associated with heart failure rather than with other clinical traits such as sex, age, smoking and diabetes mellitus. Moreover, the module was significantly correlated with certain indicators of left ventricular function like ejection fraction and left ventricular end-diastolic diameter, implying the potential of its components as crucial biomarkers. Apart from enhancer-like function, lincRNAs in this module could act as competing endogenous RNAs (ceRNAs) to regulate genes which were associated with left-ventricular systolic function. Our work provided deep insights into the critical roles of lincRNAs in the pathology of heart failure and suggested that they could be valuable biomarkers and therapeutic targets.

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“…The ECG waveforms of MI group were completely different compared to the normal ECG. The ST segment of ECG, shown in Figure 14, was noticeably elevated, suggesting that MI was successfully induced [36][37][38] and MI can be diagnosed with our device. differed slightly depending on the rats and the position of the devices, the normal rats showed normal characteristic features of rat ECG using our device [34,35].…”

Section: In Vivo Experimentsmentioning

confidence: 87%

“…The ECG waveforms of MI group were completely different compared to the normal ECG. The ST segment of ECG, shown in Figure 14, was noticeably elevated, suggesting that MI was successfully induced [36][37][38] and MI can be diagnosed with our device. Isoproterenol (30 ug/kg, 300 ul) and Diltiazem (10 mg/kg, 300 ul) were used to make change in HR.…”

Section: In Vivo Experimentsmentioning

confidence: 87%

A Wireless Power Transfer Based Implantable ECG Monitoring Device

Kim

et al. 2020

Energies

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Implantable medical devices (IMDs) enable patients to monitor their health anytime and receive treatment anywhere. However, due to the limited capacity of a battery, their functionalities are restricted, and the devices may not achieve their intended potential fully. The most promising way to solve this limited capacity problem is wireless power transfer (WPT) technology. In this study, a WPT based implantable electrocardiogram (ECG) monitoring device that continuously records ECG data has been proposed, and its effectiveness is verified through an animal experiment using a rat model. Our proposed device is designed to be of size 24 × 27 × 8 mm, and it is small enough to be implanted in the rat. The device transmits data continuously using a low power Bluetooth Low Energy (BLE) communication technology. To charge the battery wirelessly, transmitting (Tx) and receiving (Rx) antennas were designed and fabricated. The animal experiment results clearly showed that our WPT system enables the device to monitor the ECG of a heart in various conditions continuously, while transmitting all ECG data in real-time.Energies 2020, 13, 905 2 of 16 heart represented in a waveform and several features related to the cardiac cycle. Many heart diseases such as ischemic heart disease, arrhythmia, hypertrophy, and cardiomyopathies can be diagnosed by analyzing ECG waveform features [4]. However, the symptoms of arrhythmia are not continuous and appear intermittently. Thus, accurate diagnosis of AF using ECG at the specific time of monitoring would be difficult [5].To increase the accuracy of AF diagnosis, various types of ambulatory ECG monitoring devices in the form of portable, patch, wearable, and implantable to enable continuous recording have been researched and developed [6]. Each type of those devices has different advantages and disadvantages. The portable Holter monitor can measure the most comprehensive 12 leads ECG using several electrodes for 24 to 48 h. While the Holter monitors have been used in hospitals successfully, it is difficult to use them in everyday life due to short operating time and the inconvenience of wires and gel electrodes. The wearable 1 lead patch-type devices have better usability for daily life monitoring, but they also have the disadvantage of using gel electrodes or adhesives despite being non-invasive and leadless. A wearable watch type device such as Kardiaband (AliveCor, USA) for Apple Watch has been proposed recently to enhance portability and mobility. However, those watch-type devices are only capable for on-demand measurement meaning that the ECG measurement is only performed when users initiate the measurement. The ECG measures the potential difference caused by polarization and depolarization in the heart, and thus two electrodes should be placed far from each other to increase the signal quality against noise. This implies the ECG can only be measured when the user touches the electrodes with both hands. Thus, convenience and continuity of measurement are yet to be solved by these device...

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Cambios en el ECG y la actividad enzimática en corazón de rata tras infarto de miocardio: efecto de la TPP y del MnCl2

Cited by 7 publications

References 29 publications

Thiamine increases the resistance of baker's yeastSaccharomyces cerevisiaeagainst oxidative, osmotic and thermal stress, through mechanisms partly independent of thiamine diphosphate-bound enzymes

Thiamine increases the resistance of baker's yeastSaccharomyces cerevisiaeagainst oxidative, osmotic and thermal stress, through mechanisms partly independent of thiamine diphosphate-bound enzymes

Dysregulated long intergenic non-coding RNA modules contribute to heart failure

A Wireless Power Transfer Based Implantable ECG Monitoring Device

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