Cambio de CLSI a EUCAST en la interpretación de la sensibilidad a antimicrobianos: ¿cómo influye en nuestro medio?

Sánchez-Bautista, Antonia; Coy, Javier; García-Shimizu, P.; Rodrı́guez, Juan Carlos

doi:10.1016/j.eimc.2017.03.003

Cited by 10 publications

(4 citation statements)

References 15 publications

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“…Zone diameter data for first-line antimicrobial agents tested according to CLSI standards against all non-duplicate (first isolate per patient) clinical isolates of Escherichia coli , Klebsiella pneumoniae , and Pseudomonas aeruginosa from 1 January 2017 to 31 December 2017 were extracted from the Laboratory Information Management System and interpreted separately using EUCAST 2018 [2] and CLSI 2018 [3] breakpoints as susceptible, intermediate, or resistant and category agreement (percentage of isolates with the same result) determined. These organisms were selected as they were the commonest Gram-negative isolates in 2017 for which both organisations provide clinical breakpoints, and recent studies have reported discrepancies in their susceptibility interpretation [4] , [5] .…”

mentioning

confidence: 99%

Impact of CLSI and EUCAST breakpoint discrepancies on reporting of antimicrobial susceptibility and AMR surveillance

Cusack

Ashley

Ling

et al. 2019

Clinical Microbiology and Infection

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mentioning

confidence: 99%

Impact of CLSI and EUCAST breakpoint discrepancies on reporting of antimicrobial susceptibility and AMR surveillance

Cusack

Ashley

Ling

et al. 2019

Clinical Microbiology and Infection

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“…CLSI and EUCAST susceptibility profiles have been compared in studies, and significant changes have been reported concerning certain pathogens [21][22][23][24][25][26]. Due to its more restrictive breakpoints, lower susceptibility rates have been observed for EUCAST [21].…”

Section: Discussionmentioning

confidence: 99%

“…Several countries have been progressively moving to implement EUCAST as a standard AST guideline, and for S. aureus, (excluding use of aminoglycosides) some studies evaluating the impact of this change in staphylococci show excellent correlations between both guidelines. The use of EUCAST criteria for minimal inhibitory concentration (MIC) results in a significant reduction in GEN susceptibility in S. aureus [22][23][24]. One study with CoNS observed the same for S. haemolyticus but not for S. lugdunensis.…”

Section: Discussionmentioning

confidence: 99%

Coagulase-negative staphylococci in outpatient routines: the implications of switching from CLSI to BrCAST/EUCAST guidelines

Perez

Carvalho

Oliveira³

et al. 2020

Braz J Microbiol

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Coagulase-negative staphylococci (CoNS) are frequently isolated in clinical specimens and are important reservoirs of resistance genes. In 2019, the Brazilian government set the BrCAST/EUCAST (Brazilian Committee on Antimicrobial Susceptibility Testing) guidelines as the national standard, resulting in changes in the interpretation of CoNS susceptibility tests. From outpatients, disk-diffusion susceptibility of 65 CoNS cultures were evaluated and compared using classification criteria from both CLSI and BrCAST/EUCAST. The isolates were identified using matrix assisted laser desorption ionization-time of flight (MALDI-TOF), and the presence of the mecA gene was determined. The most prevalent species were Staphylococcus saprophyticus (32.3%), S. haemolyticus (18.5%), and S. epidermidis (9.2%). Almost perfect agreement was seen between the guidelines, except concerning oxacillin and gentamicin, and the prevalence of multidrug resistant isolates increased with the use of BrCAST/EUCAST. Of all, 15 (23.1%) isolates, mainly S. epidermidis and S. haemolyticus, were positive for the mecA gene, and only three were detected when using CLSI or BrCAST/EUCAST disk-diffusion screening. This, using either guideline, could reveal the difficulty of determining oxacillin resistance. Using warning zones or molecular methods might well be indicated for CoNS. In conclusion, adoption of the BrCAST/EUCAST guidelines will result in certain artificial changes in epidemiological susceptibility profiles, and clinicians and institutions should be aware of the possible implications.

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“…They had previously followed the criteria of the American Clinical and Laboratory Standard Institute (CLSI) for this purpose. Comparison of the resistance data obtained using each set of criteria may be useful in the development of a consensus on the most appropriate and clinically relevant breakpoints [5,6].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Changes in Gram-Positive Bacteria from Urine Cultures: Development Analysis in a Health Area of South-East Spain

Fernández-Espigares,

Hernández-Chico,

Expósito-Ruiz

et al. 2023

Antibiotics

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This study analyzed the epidemiology and antibiotic susceptibility profile of significant bacteriuria and assessed the impact of adopting EUCAST criteria on antibiotic resistances. A systematic review was performed on publications in English or Spanish between 1 January 2010 and 30 June 2021 on the susceptibility of Gram-positive bacteria isolated in urinary samples in Europe. A retrospective descriptive study was also conducted on the results of 21,838 urine cultures with presumptive urinary tract infection (UTI) obtained during the past five years by the Department of Microbiology of the Virgen de las Nieves University Hospital (Granada, Spain). The activity of various antibiotics was determined, differentiated among various populations, and interpretations compared according to the application of EUCAST or CLSI criteria. Among 21,838 cases of significant bacteriuria, 27.69% were by Gram-positive bacteria, which were Enterococcus faecalis in 19.04% and Enterococcus faecium in 3.92%. The susceptibility profile remained stable for most antibiotics except for levofloxacin for E. faecalis and Staphylococcus aureus and nitrofurantoin for E. faecium. The resistance of Enterococcus spp. and Staphylococcus spp. to glycopeptides was exceptionally low in our setting. No significant difference in the prevalence of methicillin-resistant Staphylococcus aureus was observed between hospital (26.67%) and community (28.85%) samples. Resistances in our local setting remain stable and appear to be lower than reported in other studies. The adoption of EUCAST vs. CLSI criteria did not produce a general change in resistance rates. Findings suggest the need to revise certain empirical criteria, such as aminoglycoside synergy for Enterococcus and for community-origin S. aureus.

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Cambio de CLSI a EUCAST en la interpretación de la sensibilidad a antimicrobianos: ¿cómo influye en nuestro medio?

Cited by 10 publications

References 15 publications

Impact of CLSI and EUCAST breakpoint discrepancies on reporting of antimicrobial susceptibility and AMR surveillance

Impact of CLSI and EUCAST breakpoint discrepancies on reporting of antimicrobial susceptibility and AMR surveillance

Coagulase-negative staphylococci in outpatient routines: the implications of switching from CLSI to BrCAST/EUCAST guidelines

Antibiotic Resistance Changes in Gram-Positive Bacteria from Urine Cultures: Development Analysis in a Health Area of South-East Spain

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