Calvin cycle multienzyme complexes are bound to chloroplast thylakoid membranes of higher plants in situ.

Süss, Karl-Heinz; Arkona, Christoph; Manteuffel, Renate; Adler, Klaus

doi:10.1073/pnas.90.12.5514

Cited by 147 publications

(58 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could be investigated further with knowledge of concentrations of other intermediates. The absence of a larger rise in G6P associated with the increase in F6P might indicate that the reaction catalysed by hexose-phosphate isomerase is not readily interacting with the Calvin cycle pool of F6P, perhaps providing further evidence for some degree of compartmentation within the chloroplast, perhaps due to the presence of Calvin cycle multi-enzyme complexes (Suss et a/., 1993).…”

Section: Discussionmentioning

confidence: 99%

Reduction in phosphoribulokinase activity by antisense RNA in transgenic tobacco: effect on CO₂ assimilation and growth in low irradiance

et al. 1995

View full text Add to dashboard Cite

control coefficient for COz assimilation of zero, and that even when amounts of PRK are reduced 20-fold relative to wild-type, altered amounts of metabolites compensate for much of the reduction in PRK protein; (ii} in plants where there is a 95% reduction in amounts of PRK, photosynthesis was reduced twofold without large changes in leaf protein content or leaf geometry. SummaryTo quantify the importance of the Calvin cycle enzyme phosphoribulokinase (PRK} in photosynthesis and to perturb photosynthesis without large direct reductions in leaf protein content, tobacco plants (Nicotiana tabacum L.) were transformed with an inverted cDNA encoding tobacco PRK. A population of plants expressing antisensa RNA and a range of PRK activities from wild-type to less than 5% of wild-type were obtained. CO2 assimilation under the growing conditions (330 Ilmol photons m -2 sec -1, 350 I~bar CO 2, 25°C) was not inhibited until more than 85% of PRK activity had been removed. With reduction in PRK activity of between 85 and 95%, assimilation rates and amounts of chlorophyll compared with wild-type were reduced by up to half. Decreased absorption of light by leaves with less chlorophyll accounted for only a small part of the reduction in assimilation rate. When PRK activity was below 15% of wild-type, amounts of ribulose-5-phosphate, ribosa-5-phosphate, ATP and fructose-6-phosphate were 1.5-to fivefold higher and levels of ribuIose-l,5-bisphosphate, 3-phosphoglyceric acid and ADP 1.5-to fourfold lower than in wild-type. It is estimated that these changes maintained flux through PRK to realise the assimilation rates observed. A possible shift of control within the Calvin cycle towards fructose-l,6-bisphosphatase in plants with low PRK is discussed. Amounts of hexoses and starch in particular were reduced in plants expressing the lowest PRK activities; amounts of sucrose were little affected. Lower CO 2 assimilation in plants with low PRK activity correlated with reduced relative growth rate of shoots and delayed flowering, but there was no effect on specific leaf area. It is concluded that (i) in wildtype plants grown in constant low light, PRK has a flux-

show abstract

Section: Discussionmentioning

confidence: 99%

Reduction in phosphoribulokinase activity by antisense RNA in transgenic tobacco: effect on CO₂ assimilation and growth in low irradiance

et al. 1995

View full text Add to dashboard Cite

show abstract

“…Reports over several decades have noted different combinations of Calvin cycle protein complexes, including early reports of associations between Rubisco with other Calvin cycle enzymes and ferredoxin reductase (Suss et al, 1993). Additionally, there are detailed analyses of the role of CP12 in complexing with and modifying the activity of the Calvin cycle enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (Wedel et al, 1997;Carmo-Silva et al, 2011;Gontero and Maberly, 2012) as well as a recent report of megadalton complexes of GAPDH with small and large subunits of Rubisco and 400-kD complexes of Rubisco, GAPDH, and phosphoribulokinase (Behrens et al, 2013).…”

Section: Calvin Cyclementioning

confidence: 99%

Proteins with High Turnover Rate in Barley Leaves Estimated by Proteome Analysis Combined with in Planta Isotope Labeling

et al. 2014

View full text Add to dashboard Cite

Protein turnover is a key component in cellular homeostasis; however, there is little quantitative information on degradation kinetics for individual plant proteins. We have used 15 N labeling of barley (Hordeum vulgare) plants and gas chromatography-mass spectrometry analysis of free amino acids and liquid chromatography-mass spectrometry analysis of proteins to track the enrichment of 15 N into the amino acid pools in barley leaves and then into tryptic peptides derived from newly synthesized proteins. Using information on the rate of growth of barley leaves combined with the rate of degradation of 14 N-labeled proteins, we calculate the turnover rates of 508 different proteins in barley and show that they vary by more than 100-fold. There was approximately a 9-h lag from label application until 15 N incorporation could be reliably quantified in extracted peptides. Using this information and assuming constant translation rates for proteins during the time course, we were able to quantify degradation rates for several proteins that exhibit half-lives on the order of hours. Our workflow, involving a stringent series of mass spectrometry filtering steps, demonstrates that 15 N labeling can be used for large-scale liquid chromatography-mass spectrometry studies of protein turnover in plants. We identify a series of abundant proteins in photosynthesis, photorespiration, and specific subunits of chlorophyll biosynthesis that turn over significantly more rapidly than the average protein involved in these processes. We also highlight a series of proteins that turn over as rapidly as the well-known D1 subunit of photosystem II. While these proteins need further verification for rapid degradation in vivo, they cluster in chlorophyll and thiamine biosynthesis.

show abstract

“…For example, the Calvin cycle occurs in the stroma, which is analogous to the cytoplasm of a cell (Suss et al, 1993). The light-dependent reactions occur within the membranes of an elaborate network of flattened vesicles, the thylakoids.…”

Section: Introductionmentioning

confidence: 99%

Photosystem II Assembly and Repair Are Differentially Localized inChlamydomonas

Uniacke

Zerges

2007

The Plant Cell

View full text Add to dashboard Cite

Many proteins of the photosynthesis complexes are encoded by the genome of the chloroplast and synthesized by bacteriumlike ribosomes within this organelle. To determine where proteins are synthesized for the de novo assembly and repair of photosystem II (PSII) in the chloroplast of Chlamydomonas reinhardtii, we used fluorescence in situ hybridization, immunofluorescence staining, and confocal microscopy. These locations were defined as having colocalized chloroplast mRNAs encoding PSII subunits and proteins of the chloroplast translation machinery specifically under conditions of PSII subunit synthesis. The results revealed that the synthesis of the D1 subunit for the repair of photodamaged PSII complexes occurs in regions of the chloroplast with thylakoids, consistent with the current model. However, for de novo PSII assembly, PSII subunit synthesis was detected in discrete regions near the pyrenoid, termed T zones (for translation zones). In two PSII assembly mutants, unassembled D1 subunits and incompletely assembled PSII complexes localized around the pyrenoid, where we propose that they mark an intermediate compartment of PSII assembly. These results reveal a novel chloroplast compartment that houses de novo PSII biogenesis and the regulated transport of newly assembled PSII complexes to thylakoid membranes throughout the chloroplast.

show abstract

Calvin cycle multienzyme complexes are bound to chloroplast thylakoid membranes of higher plants in situ.

Abstract: Further evidence is provided that the Calvin cycle enzymes ribose-5-phosphate isomerase (EC 5.3

Cited by 147 publications

References 17 publications

Reduction in phosphoribulokinase activity by antisense RNA in transgenic tobacco: effect on CO₂ assimilation and growth in low irradiance

Reduction in phosphoribulokinase activity by antisense RNA in transgenic tobacco: effect on CO₂ assimilation and growth in low irradiance

Proteins with High Turnover Rate in Barley Leaves Estimated by Proteome Analysis Combined with in Planta Isotope Labeling

Photosystem II Assembly and Repair Are Differentially Localized inChlamydomonas

Contact Info

Product

Resources

About

Calvin cycle multienzyme complexes are bound to chloroplast thylakoid membranes of higher plants in situ.

Abstract: Further evidence is provided that the Calvin cycle enzymes ribose-5-phosphate isomerase (EC 5.3

Cited by 147 publications

References 17 publications

Reduction in phosphoribulokinase activity by antisense RNA in transgenic tobacco: effect on CO2 assimilation and growth in low irradiance

Reduction in phosphoribulokinase activity by antisense RNA in transgenic tobacco: effect on CO2 assimilation and growth in low irradiance

Proteins with High Turnover Rate in Barley Leaves Estimated by Proteome Analysis Combined with in Planta Isotope Labeling

Photosystem II Assembly and Repair Are Differentially Localized inChlamydomonas

Contact Info

Product

Resources

About

Reduction in phosphoribulokinase activity by antisense RNA in transgenic tobacco: effect on CO₂ assimilation and growth in low irradiance

Reduction in phosphoribulokinase activity by antisense RNA in transgenic tobacco: effect on CO₂ assimilation and growth in low irradiance