Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials

Marger, Laurine; Barone, Antonio; Martinelli-Kläy, Carla Patrícia; Schaub, Leandra; Strasding, Malin; Mekki, Mustapha; Sailer, Irena; Scherrer, Susanne S.; Durual, Stéphane

doi:10.3791/59976

Cited by 4 publications

(10 citation statements)

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“…), 2–8 reactors may be fixed on the skull. The rabbit appears as a rationale choice, first because of quite similitudes with human bone metabolism and structures [ 28 ] and secondly for practical reasons as four reactors may be placed simultaneously [ 25 , 29 ]. As a proof of the relevancy of this animal model for bone regeneration, about 80% of the references based on the calvarial model used rabbits.…”

Section: Discussionmentioning

confidence: 99%

“…In vivo testing was done according to the recently developed rabbit calvarial model [ 25 ]. Four individual cylinders were placed on rabbit skulls, on anatomical locations defined by the crossing of the median and coronal sutures (occipital left (OL) and right (PR), frontal left (FL) and right (FR)).…”

Section: Methodsmentioning

confidence: 99%

“…The model was thorough described previously [ 25 ]. Briefly, animals underwent: Pre-anesthesia, im injection: ketamine (25 mg/kg, 50 mg/mL, 0.5 mL/kg, Pfizer, NY, USA) plus xylazin (3 mg/kg, 20 mg/mL, 0.15 mL/kg, Bayer).…”

Section: Methodsmentioning

confidence: 99%

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Pre-Treat Xenogenic Collagenous Blocks of Bone Substitutes with Saline Facilitate Their Manipulation and Guarantee High Bone Regeneration Rates, Qualitatively and Quantitatively

et al. 2021

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Deproteinized bovine bone mineral particles embedded in collagen (DBBM-C) are widely used for bone regenerations with excellent, albeit sometimes variable clinical outcomes. Clinicians usually prepare DBBM-C by mixing with blood. Replacing blood by saline represents an alternative. We investigated if saline treatment could improve DBBM-C i. handling in vitro and ii. biological performances in a rabbit calvarial model. In vitro, DBBM-C blocks soaked in saline or blood were submitted to compression tests. In vivo, four poly ether ether ketone (PEEK)cylinders were placed on 16 rabbit skulls, filled with DBBM-C soaked in blood or saline for 2–4–8–12 weeks before histomorphometry. DBBM-C blocks were fully hydrated after 30 s in saline when 120 s in blood could not hydrate blocks core. Stiffness gradually decreased 2.5-fold after blood soaking whereas a six-fold decrease was measured after 30 s in saline. In vivo, saline treatment allowed 50% more bone regeneration during the first month when compared to blood soaking. This difference was then no longer visible. New bone morphology and maturity were equivalent in both conditions. DBBM-C saline-soaking facilitated its handling and accelerated bone regeneration of highly qualitative tissues when compared to blood treatment. Saline pretreatment thus may increase the clinical predictability of bone augmentation procedures.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Pre-Treat Xenogenic Collagenous Blocks of Bone Substitutes with Saline Facilitate Their Manipulation and Guarantee High Bone Regeneration Rates, Qualitatively and Quantitatively

et al. 2021

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“…From a developmental perspective, the calvaria and jaw are plate-like bones with embryonic homology (34). However, from a translational perspective, the calvaria is similar to an atrophic mandible (35); therefore, animal calvaria may be used as an experimental site to test bone materials for vertical bone augmentation (36,37). The advantages of the calvaria model include adequate surgical access, a clear surgical field of view, ease of operation, uncomplicated postoperative care and good reproducibility (38).…”

Section: Different Experimental Models Of Vertical Bone Augmentationmentioning

confidence: 99%

“…The VGBR model of rabbit calvaria is similar to that developed for rats. Marger et al (35) described the detailed protocol for using rabbit calvaria as a VGBR model. The surface area of the rabbit calvaria is larger compared with that of the rat and up to four experimental groups may be compared in the same animal (58,59).…”

Section: Different Experimental Models Of Vertical Bone Augmentationmentioning

confidence: 99%

Animal models of vertical bone augmentation (Review)

et al. 2021

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Vertical bone augmentation is an important challenge in dental implantology. Existing vertical bone augmentation techniques, along with bone grafting materials, have achieved certain clinical progress but continue to have numerous limitations. In order to evaluate the possibility of using biomaterials to develop bone substitutes, medical devices and/or new bone grafting techniques for vertical bone augmentation, it is essential to establish clinically relevant animal models to investigate their biocompatibility, mechanical properties, applicability and safety. The present review discusses recent animal experiments related to vertical bone augmentation. In addition, surgical protocols for establishing relevant preclinical models with various animal species were reviewed. The present study aims to provide guidance for selecting experimental animal models of vertical bone augmentation. Contents 1. Introduction 2. Different experimental models of vertical bone augmentation 3. Discussion 4. Conclusion

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A novel pilot animal model for bone augmentation using osseous shell technique for preclinical in vivo studies

Kamal

Al‐Obaidly

Lethaus

et al. 2022

Clinical & Exp Dental Res

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Objectives: Bone grafting is commonly used to reconstruct skeletal defects in the craniofacial region. Several bone augmentation models have been developed to evaluate bone formation using novel bone substitute materials. The aim of this study was to evaluate a surgical animal model for establishing a three-dimensional (3D) grafting environment in the animal's mandibular ramus for bone augmentation using the osseous shell technique, as in humans.Materials and Methods: Osteological survey of New Zealand white (NZW) rabbit skull (Oryctolagus cuniculus): Initial osteological and imaging surveys were performed on a postmortem skull for a feasibility assessment of the surgical procedure.Postmortem pilot surgery and cone beam computed tomography imaging: a 3D osseous defect was created in the mandibular ramus through a submandibular incision. The osseous shell plates were stabilized with osteosynthesis fixation screws, and defects were filled with particular bone grafting material. In vivo surgical procedure: surgeries were conducted in four 8-week-old NZW rabbits utilizing two osseous shell materials: xenogeneic human cortical plates and autogenous rabbit cortical plates. The created 3D defects were filled using xenograft and allograft bone grafting materials. The healed defects were evaluated for bone formation after 12 weeks using histological and cone beam computed tomography imaging analysis.Results: Clinical analysis 12 weeks after surgery revealed the stability of the 3D grafted bone augmentation defects using the osseous shell technique. Imaging and histological analyses confirmed the effectiveness of this model in assessing bone formation. Conclusions:The proposed animal model is a promising model with the potential to study various bone grafting materials for augmentation in the mandibular ramus using the osseous shell technique without compromising the health of the animal.The filled defects could be analyzed for osteogenesis, quantification of bone formation, and healing potential using histomorphometric analysis, in addition to 3D morphologic evaluation using radiation imaging.

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Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials

Cited by 4 publications

References 0 publications

Pre-Treat Xenogenic Collagenous Blocks of Bone Substitutes with Saline Facilitate Their Manipulation and Guarantee High Bone Regeneration Rates, Qualitatively and Quantitatively

Pre-Treat Xenogenic Collagenous Blocks of Bone Substitutes with Saline Facilitate Their Manipulation and Guarantee High Bone Regeneration Rates, Qualitatively and Quantitatively

Animal models of vertical bone augmentation (Review)

A novel pilot animal model for bone augmentation using osseous shell technique for preclinical in vivo studies

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