Calsyntenin 1 mRNA expression sensitivity to ionizing radiation in human hepatocytes and carcinoma cells and blood cells of BALB/c mice

Lee, Eon-Seok; Kim, Won‐Tae; Park, Ga-Young; Lee, Manwoo; Son, Tae Gen

doi:10.1080/16878507.2020.1855911

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…We observed no increase in CLSTN1 transcripts after 6 or 24h of prostetin/12K treatment, suggesting that CLSTN1 protein expression is controlled by MAP4K via a posttranscriptional mechanism. A study in whole mice and lung adenocarcinoma cells found that CLSTN1 expression is decreased in response to 4 Gy gamma irradiation 36 . We previously observed an increased MAP4K4-dependent invasiveness in SHH MB tumor cells exposed to 1 – 2 Gy 37 , suggesting that the irradiation-induced increase in cell invasion observed could in part be mediated by CLSTN1 downregulation via increased MAP4K4 activity.…”

Section: Discussionmentioning

confidence: 99%

MAP4 kinase-regulated reduced CLSTN1 expression in medulloblastoma is associated with increased invasiveness

Sönmez,

Yan,

Lin

et al. 2024

Preprint

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De-regulated protein expression contributes to tumor growth and progression in medulloblastoma (MB), the most common malignant brain tumor in children. MB is associated with impaired differentiation of specific neural progenitors, suggesting that the deregulation of proteins involved in neural physiology could contribute to the transformed phenotype in MB. Calsynthenin 1 (CLSTN1) is a neuronal protein involved in cell-cell interaction, vesicle trafficking, and synaptic signaling. We previously identified CLSTN1 as a putative target of the pro-invasive kinase MAP4K4, which we found to reduce CLSTN1 surface expression. Herein, we explored the expression and functional significance of CLSTN1 in MB. We found that CLSTN1 expression is decreased in primary MB tumors compared to tumor-free cerebellum or brain tissues. CLSTN1 is expressed in laboratory-established MB cell lines, where it localized to the plasma membrane, intracellular vesicular structures, and regions of cell-cell contact. The reduction of CLSTN1 expression significantly increased growth factor-driven invasiveness. Pharmacological inhibition of MAP4 kinases caused increased CLSTN1 expression and CLSTN1 accumulation in cell-cell contacts. Co-culture of tumor cells with astrocytes increased CLSTN1 localization in cell-cell contacts, which was further enhanced by MAP4K inhibition. Our study revealed a repressive function of CLSTN1 in growth-factor-driven invasiveness in MB and its potential implication in homologous and heterologous interactions of the tumor cells with cells in their microenvironment.

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Section: Discussionmentioning

confidence: 99%

MAP4 kinase-regulated reduced CLSTN1 expression in medulloblastoma is associated with increased invasiveness

Sönmez,

Yan,

Lin

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…We observed no increase in CLSTN1 transcripts after 6 or 24h of prostetin/12K treatment, suggesting that CLSTN1 protein expression is controlled by MAP4K via a posttranscriptional mechanism. A study in whole mice and lung adenocarcinoma cells found that CLSTN1 expression is decreased in response to 4 Gy gamma irradiation 35 . We previously observed an increased MAP4K4-dependent invasiveness in SHH MB tumor cells exposed to 1 -2 Gy 36 , suggesting that the irradiation-induced increase in cell invasion observed could in part be mediated by CLSTN1 downregulation via increased MAP4K4 activity.…”

Section: Discussionmentioning

confidence: 99%

MAP4 kinase regulated reduced CLSTN1 expression in medulloblastoma is associated with increased invasiveness

Sönmez,

Yan,

Lin

et al. 2024

Preprint

View full text Add to dashboard Cite

De-regulated protein expression contributes to tumor growth and progression in medulloblastoma (MB), the most common malignant brain tumor in children. MB is associated with impaired differentiation of specific neural progenitors, suggesting that the deregulation of proteins involved in neural physiology could contribute to the transformed phenotype in MB. Calsynthenin 1 (CLSTN1) is a neuronal protein involved in cell-cell interaction, vesicle trafficking, and synaptic signaling. Herein, we explored the expression and functional significance of CLSTN1 in MB. We found that CLSTN1 expression is decreased in primary MB tumors compared to tumor-free cerebellum or brain tissues. CLSTN1 is expressed in laboratory-established MB cell lines, where it localized to the plasma membrane, intracellular vesicular structures, and regions of cell-cell contact. The reduction of CLSTN1 expression significantly increased growth factor-driven invasiveness. Pharmacological inhibition of MAP4 kinases caused increased CLSTN1 expression and CLSTN1 accumulation in cell-cell contacts. Co-culture of tumor cells with astrocytes increased CLSTN1 localization in cell-cell contacts, which was further enhanced by MAP4K inhibition. Our study revealed a repressive function of CLSTN1 in growth-factor-driven invasiveness in MB and its potential implication in homologous and heterologous interactions of the tumor with cells in its microenvironment.

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Design and applications of anti-management model in China’s primary refineries

Xue

Chen

Chunyun

et al. 2021

Journal of Radiation Research and Applied Sciences

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To overcome the weak control of the problem of fragmentation and surface layering on the risk monitoring of the refinery, and to improve the safety management level in the petroleum refining process, The research team decomposes the potential risks of the oil refining system into 5 systems such as 'personnel, facilities, materials, technology, and environment' and 106 safety control points, uses semi-quantitative analysis to derive safety evaluation index values, and uses weighted evaluation to derive refining The overall safety assessment of system risks, and the design of an 'anti-management' safety inspection model, focuses on key safety hazards to eliminate hidden accidents. The research model was applied to an oil refinery and two rounds of safety evaluation were carried out. The results were G1 = 84.406, G2 = 91.400, G2> G1, indicating that personnel, machinery and technology have been improved significantly, whereas fuel and environment have been improved slowly, and personnel remained safe. The greatest improvement in quality proves the feasibility of the anti-management risk control model, and its significant issue processing method has a key role. It can provide theoretical models and assistance for the advancement of safety management of petroleum refining and chemical enterprises.

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Calsyntenin 1 mRNA expression sensitivity to ionizing radiation in human hepatocytes and carcinoma cells and blood cells of BALB/c mice

Cited by 3 publications

References 24 publications

MAP4 kinase-regulated reduced CLSTN1 expression in medulloblastoma is associated with increased invasiveness

MAP4 kinase-regulated reduced CLSTN1 expression in medulloblastoma is associated with increased invasiveness

MAP4 kinase regulated reduced CLSTN1 expression in medulloblastoma is associated with increased invasiveness

Design and applications of anti-management model in China’s primary refineries

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