Calsensin: a novel calcium-binding protein expressed in a subset of peripheral leech neurons fasciculating in a single axon tract.

Briggs, Kristen K.; Silvers, Andrea J.; Johansen, Kristen M.

doi:10.1083/jcb.129.5.1355

Cited by 13 publications

(3 citation statements)

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“…A particularly advantageous system in which to study the potential function of specific oligosaccharide modifications of neural proteins regulating pathway formation is in the projections of sensillar neurons in leech. Using mAbs and dye injections, the axons of sensillar neurons have been shown to project in tightly fasciculated bundles through the periphery into the central nervous system (CNS) where they bifurcate and segregate into four well-defined and stereotypically located fascicles in each of the central connectives ( Johansen et al, 1992 ; Jellies et al, 1994 ; Briggs et al, 1995 ). At least five different mAbs (Lan3-2, Lan2-3, Lan4-2, Laz2-369, Laz7-79) that recognize different glycoepitopes specific to the entire population as well as distinct subsets of these neurons have been identified ( McKay et al, 1983 ; Peinado et al, 1987 ; Bajt et al, 1990 ; Johansen et al, 1992 ; Zipser et al, 1994 ).…”

mentioning

confidence: 99%

Differential Glycosylation of Tractin and LeechCAM, Two Novel Ig Superfamily Members, Regulates Neurite Extension and Fascicle Formation

Huang

Jellies²,

Johansen

1997

The Journal of Cell Biology

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By immunoaffinity purification with the mAb Lan3-2, we have identified two novel Ig superfamily members, Tractin and LeechCAM. LeechCAM is an NCAM/FasII/ApCAM homologue, whereas Tractin is a cleaved protein with several unique features that include a PG/YG repeat domain that may be part of or interact with the extracellular matrix. Tractin and LeechCAM are widely expressed neural proteins that are differentially glycosylated in sets and subsets of peripheral sensory neurons that form specific fascicles in the central nervous system. In vivo antibody perturbation of the Lan3-2 glycoepitope demonstrates that it can selectively regulate extension of neurites and filopodia. Thus, these experiments provide evidence that differential glycosylation can confer functional diversity and specificity to widely expressed neural proteins.

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confidence: 99%

Differential Glycosylation of Tractin and LeechCAM, Two Novel Ig Superfamily Members, Regulates Neurite Extension and Fascicle Formation

Huang

Jellies²,

Johansen

1997

The Journal of Cell Biology

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“…Calsensin is a EF-hand calcium-binding protein that was first found in the leech Haemopis marmorata , and is thought to mediate calcium-dependant signal transduction events in growth cones and axones of the developing nervous system [ 35 ]. The spatial expression of an EF-hand calcium-binding protein Calsensin ortholog in A. lata ( Ala-calsensin ) has been characterized, appearing to be expressed in the segmental ganglia and peripheral neurons in the body wall during organogenesis (10 and 11).…”

Section: Discussionmentioning

confidence: 99%

Developmental biology and potential use of Alboglossiphonia lata (Annelida: Hirudinea) as an “Evo-Devo” model organism

et al. 2017

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BackgroundThe need for the adaptation of species of annelids as “Evo-Devo” model organisms of the superphylum Lophotrochozoa to refine the understanding of the phylogenetic relationships between bilaterian organisms, has promoted an increase in the studies dealing with embryonic development among related species such as leeches from the Glossiphoniidae family. The present study aims to describe the embryogenesis of Alboglossiphonia lata (Oka, 1910), a freshwater glossiphoniid leech, chiefly distributed in East Asia, and validate standard molecular biology techniques to support the use of this species as an additional model for “Evo-Devo” studies.Results A. lata undergoes direct development, and follows the highly conserved clitellate annelid mode of spiral cleavage development; the duration from the egg laying to the juvenile stage is ~7.5 days, and it is iteroparous, indicating that it feeds and deposits eggs again after the first round of brooding, as described in several other glossiphoniid leech species studied to date. The embryos hatch only after complete organ development and proboscis retraction, which has not yet been observed in other glossiphoniid genera. The phylogenetic position of A. lata within the Glossiphoniidae family has been confirmed using cytochrome c oxidase subunit 1 (CO1) sequencing. Lineage tracer injections confirmed the fates of the presumptive meso- and ectodermal precursors, and immunostaining showed the formation of the ventral nerve system during later stages of development. Further, the spatiotemporal expression of an EF-hand calcium-binding protein Calsensin ortholog was characterized, which showed a specific pattern in both the ventral and peripheral nervous systems during the later stages.ConclusionsOur description of the embryonic development of A. lata under laboratory conditions provides new data for further comparative studies with other leech and lophotrochozoa model organisms. Moreover, it offers a basis for the establishment of this species as a model for future “Evo-Devo” studies.Electronic supplementary materialThe online version of this article (10.1186/s12983-017-0240-y) contains supplementary material, which is available to authorized users.

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“…[1] applied this approach to the nervous system of the leech and identified several hundred such hybridoma lines. An advantage of this approach is that mAbs to unknown proteins that serve as markers for specific subsets of neurons [2][3][4] or for particular subcellular structures [5] of interest can readily be identified, molecularly cloned, and their amino acid sequence analyzed. The leech is a particularly advantageous organism in which to pursue such studies.…”

Section: Resultsmentioning

confidence: 99%

Molecular and functional analysis of Drosophila Hillarin and its leech homolog

Ji¹

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Calsensin: a novel calcium-binding protein expressed in a subset of peripheral leech neurons fasciculating in a single axon tract.

Cited by 13 publications

References 38 publications

Differential Glycosylation of Tractin and LeechCAM, Two Novel Ig Superfamily Members, Regulates Neurite Extension and Fascicle Formation

Differential Glycosylation of Tractin and LeechCAM, Two Novel Ig Superfamily Members, Regulates Neurite Extension and Fascicle Formation

Developmental biology and potential use of Alboglossiphonia lata (Annelida: Hirudinea) as an “Evo-Devo” model organism

Molecular and functional analysis of Drosophila Hillarin and its leech homolog

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