Calretinin staining pattern aids in the differentiation of mesothelioma from adenocarcinoma in serous effusions

Chhieng, David C.; Yee, Herman; Schaefer, Dawn; Cangiarella, Joan; Jagirdar, Jaishree; Chiriboga, Luis; Cohen, J

doi:10.1002/1097-0142(20000625)90:3<194::aid-cncr8>3.0.co;2-k

Cited by 70 publications

(49 citation statements)

References 37 publications

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“…Ovarian sex cord-stromal neoplasms are heterogeneous including granulosa cell tumors (GCTs), SertoliLeydig cell tumors, thecomas, and fibrothecomas, representing approximately 8% of ovarian neoplasms. Characteristic positive staining pattern was nuclear and cytoplasmic staining with "fried egg" appearance (9). Nonspecific cytoplasmic staining without nuclear staining as observed in the present case has also been recognized in lung and breast adenocarcinomas which metastasized to the pleural space (9).…”

Section: Discussionsupporting

confidence: 75%

Immunohistochemistry for the Differentiation of Peritoneal Disseminated Carcinoma of Unknown Origin

et al. 2004

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Section: Discussionsupporting

confidence: 75%

Immunohistochemistry for the Differentiation of Peritoneal Disseminated Carcinoma of Unknown Origin

et al. 2004

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“…[1718] Some authors have recommended single antibodies, mostly calretinin. [21223031] We do not agree with the use of a single monoclonal antibody for this purpose, which has therapeutic as well as prognostic implications. This is in agreement with other authors.…”

Section: Discussionmentioning

confidence: 81%

Utility of a limited panel of calretinin and Ber-EP4 immunocytochemistry on cytospin preparation of serous effusions: A cost-effective measure in resource-limited settings

et al. 2011

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Background: Differentiation between reactive, but morphologically atypical, mesothelial cells and adenocarcinoma in effusions can be problematic. Elaborate immunohistochemical panels have been devised. Techniques like DNA analysis, flow/image cytometry, and K-ras mutation analysis are research oriented and difficult to perform in routine, especially in resource-poor centers. We evaluated the efficacy of a limited two-antibody panel comprising calretinin and Ber-EP4 on cytospin and cell block preparations, in 100 effusion samples.Materials and Methods: Fifty cases of reactive mesothelial hyperplasia and 50 cases of adenocarcinoma diagnosed by cytomorphology in ascitic/pleural fluid specimens over a 2-year period were assessed. The diagnoses were confirmed by clinical/histopathologic correlation. Cytospin smears were made in all. Cell blocks were prepared, wherever adequate fluid was available. Immunocytochemistry (ICC) for calretinin and Ber-EP4 was performed.Results: Forty-five of the reactive effusion cases (90%) were calretinin reactive and Ber-EP4 negative. Among the adenocarcinoma cases, 49 (98%) were calretinin negative but Ber-EP4 positive. Thus, both calretinin and Ber-EP4 had a high sensitivity (90% and 98%, respectively), as well as a high specificity (100% and 86%, respectively). In the 21 reactive mesothelial cases, whose cell blocks were made, results were comparable to those on cytospin. However, of the 19 adenocarcinoma cases in which cell blocks were prepared, all were Ber-EP4 immunopositive except for three, which were positive on cytospin, implying false-negative results on cell blocks.Conclusions: A limited panel of two monoclonal antibodies, calretinin and Ber-EP4, may be useful in cytology, as a “primary antibody panel”, for accurate diagnosis and patient management. Additionally, ICC can be performed easily on cytospin preparations, which gave results comparable to cell blocks in our study.

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“…CA 19-9 may also be demonstrated in lining of the cyst wall as was demonstrated in our case 4. Calretinin is a vitamin D-dependent calcium-binding protein that is expressed in mesothelial cell lines; even benign mesothelial cells may show the characteristic “fried-egg” staining pattern 10. Cytokeratins specifically permit identification of the epithelial origin based on the expression profile.…”

Section: Discussionmentioning

confidence: 99%

Mesothelial cyst of the spleen in an adult: a case report

Vijayaraghavan¹,

Chandrashekar²,

Aithal³

et al. 2010

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SummaryA young adult in her third decade presented with a 2-week history of catching left upper abdominal pain and was detected to have a cystic lesion occupying almost the entire spleen. Laparoscopic total splenectomy was carried out, and the cyst wall revealed a true mesothelial cyst with no squamous metaplasia. The various aspects of mesothelial cysts, including immunophenotyping and treatment modalities, are briefly discussed. BACKGROUND

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Calretinin staining pattern aids in the differentiation of mesothelioma from adenocarcinoma in serous effusions

Cited by 70 publications

References 37 publications

Immunohistochemistry for the Differentiation of Peritoneal Disseminated Carcinoma of Unknown Origin

Immunohistochemistry for the Differentiation of Peritoneal Disseminated Carcinoma of Unknown Origin

Utility of a limited panel of calretinin and Ber-EP4 immunocytochemistry on cytospin preparation of serous effusions: A cost-effective measure in resource-limited settings

Mesothelial cyst of the spleen in an adult: a case report

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