Calretinin expression in tumors of adipose tissue

Cates, Justin M.; Coffing, Bryan N.; Harris, Brent T.; Black, Candice C.

doi:10.1016/j.humpath.2005.11.006

Cited by 14 publications

(18 citation statements)

References 37 publications

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“…Calretinin-positive nuclear and cytoplasmic staining was reported in lipomas and their variants, and in well-differentiated liposarcomas, at least focally. 1 Other studies demonstrate calretinin positivity in thymoma and thymic carcinoma, 20 schwannoma, 4 and high-grade breast carcinomas 9 among others.…”

Section: Discussionmentioning

confidence: 92%

“…In addition, calretinin is also a marker for sex cord stromal tumors 11 and a potential marker for adipose tissue tumors. 1 It has also been suggested as being useful in the separation of neurofibroma from schwannoma, as only the latter is typically positive. 4 We have serendipitously encountered patients with chest wall desmoid fibromatosis presented as mesothelioma, on the basis of immunohistochemical calretinin positivity.…”

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confidence: 99%

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Immunoreactivity for Calretinin and Keratins in Desmoid Fibromatosis and Other Myofibroblastic Tumors

Barak

Wang

Miettinen

2012

American Journal of Surgical Pathology

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Calretinin is an intracellular calcium-binding EF-hand protein of the calmodulin superfamily. It plays a role in diverse cellular functions, including message targeting and intracellular calcium signaling. It is expressed in the mesothelium, mast cells, some neural cells, and fat cells, among others. Because of its relative specificity for mesothelial neoplasms, calretinin is widely used as one of the primary immunohistochemical markers for malignant mesothelioma and in differentiating it from adenocarcinoma. On the basis of our sporadic observation on calretinin immunoreactivity in desmoid fibromatosis, we systematically evaluated calretinin, keratin cocktail (AE1/AE3), and WT1 immunoreactivity in 268 fibroblastic/myofibroblastic neoplasms. Calretinin was observed in 75% (44/58) of desmoid fibromatosis, 50% (21/42) of proliferative fasciitis, 23% (8/35) of nodular fasciitis, 33% (13/40) of benign fibrous histiocytoma, 35% (22/62) of malignant fibrous histiocytoma, and 13% (4/31) of solitary fibrous tumors but not in normal connective tissue fibroblasts at various sites. Keratin AE1/AE3 immunoreactivity was also commonly (6/13) present in the large ganglion-like cells of proliferative fasciitis and sometimes in nodular fasciitis (3/35), solitary fibrous tumor (3/27), and malignant fibrous histiocytoma (9/62). Nuclear immunoreactivity for WT1 or keratin 5 positivity was not detected in myofibroblastic tumors. On the basis of these observations, it can be concluded that calretinin and focal keratin immunoreactivity is fairly common in benign and malignant fibroblastic and myofibroblastic lesions. Calretinin-positive and keratin-positive spindle cells in desmoid and nodular fasciitis or calretinin-positive ganglion-like cells in proliferative fasciitis should not be confused with elements of epithelioid or sarcomatoid mesothelioma. These diagnostic pitfalls can be avoided with careful observation of morphology, quantitative differences in keratin expression, and use of additional immunohistochemical markers such keratin 5 and WT1 to verify true epithelial and mesothelial differentiation typical of mesothelioma.

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Section: Discussionmentioning

confidence: 92%

mentioning

confidence: 99%

Immunoreactivity for Calretinin and Keratins in Desmoid Fibromatosis and Other Myofibroblastic Tumors

Barak

Wang

Miettinen

2012

American Journal of Surgical Pathology

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“…Several CaBPs, including calbindin D28k, calretinin and parvalbumin, have been found in high concentrations in the brain (Baimbridge et al, 1982; García-Segura et al, 1984). CaBPs deficits have been related to relevant neurodegenerative processes, such as Alzheimer's disease, Parkinson's disease, age-related cognitive defects, schizophrenia, epilepsy, and some forms of tumors (Maglóczky et al, 1997; Cates et al, 2006; Nakazawa et al, 2012; Verret et al, 2012). These CaBPs usually correlate with neurotransmitter content, cell morphology, distribution, and function (Baimbridge et al, 1982; Celio, 1990; Gulyás et al, 1991) and are used to classify neurons into specific subpopulations.…”

Section: Introductionmentioning

confidence: 99%

Localization of peroxisome proliferator-activated receptor alpha (PPARÎ±) and N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) in cells expressing the Ca2+-binding proteins calbindin, calretinin, and parvalbumin in the adult rat hippocampus

et al. 2014

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The N-acylethanolamines (NAEs), oleoylethanolamide (OEA) and palmithylethanolamide (PEA) are known to be endogenous ligands of PPARα receptors, and their presence requires the activation of a specific phospholipase D (NAPE-PLD) associated with intracellular Ca2+ fluxes. Thus, the identification of a specific population of NAPE-PLD/PPARα-containing neurons that express selective Ca2+-binding proteins (CaBPs) may provide a neuroanatomical basis to better understand the PPARα system in the brain. For this purpose, we used double-label immunofluorescence and confocal laser scanning microscopy for the characterization of the co-existence of NAPE-PLD/PPARα and the CaBPs calbindin D28k, calretinin and parvalbumin in the rat hippocampus. PPARα expression was specifically localized in the cell nucleus and, occasionally, in the cytoplasm of the principal cells (dentate granular and CA pyramidal cells) and some non-principal cells of the hippocampus. PPARα was expressed in the calbindin-containing cells of the granular cell layer of the dentate gyrus (DG) and the SP of CA1. These principal PPARα+/calbindin+ cells were closely surrounded by NAPE-PLD+ fiber varicosities. No pyramidal PPARα+/calbindin+ cells were detected in CA3. Most cells containing parvalbumin expressed both NAPE-PLD and PPARα in the principal layers of the DG and CA1/3. A small number of cells containing PPARα and calretinin was found along the hippocampus. Scattered NAPE-PLD+/calretinin+ cells were specifically detected in CA3. NAPE-PLD+ puncta surrounded the calretinin+ cells localized in the principal cells of the DG and CA1. The identification of the hippocampal subpopulations of NAPE-PLD/PPARα-containing neurons that express selective CaBPs should be considered when analyzing the role of NAEs/PPARα-signaling system in the regulation of hippocampal functions.

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“…11,37,[46][47][48][49][50][51] In published studies by this author, calretinin expression was demonstrated in 4% to 8% of lung adenocarcinomas, 19,24 but in none of the renal cell carcinomas investigated. It should be kept in mind, however, that calretinin can also be expressed in other neoplasms with which sarcomatoid mesotheliomas can potentially be confused, including sarcomatoid carcinoma, 55,56,60 synovial sarcoma, 23 desmoid fibromatosis, 55,66 Schwannoma, 67,68 and malignant peripheral nerve sheath tumor. 52 Other carcinomas that have been reported to be calretinin positive include squamous cell carcinomas of the lung (23% to 40%) 11,19,35,48,53 and small cell lung carcinomas (41% to 49%), 35,48 tumors that, when they involve the pleura, can potentially be confused with mesothelioma.…”

Section: Calretinin As a Mesothelial Markermentioning

confidence: 99%

“…49 These findings indicate that, when used in conjunction with TTF-1 and/or napsin A, 2 markers that are highly sensitive and specific for lung adenocarcinomas, or PAX 8 and/or PAX 2, 2 renal cell carcinoma-associated markers that are absent in mesotheliomas, calretinin can assist in distinguishing epithelioid mesotheliomas from lung adenocarcinomas and renal cell carcinomas involving the pleura, respectively. 23,55,68 In addition to mesotheliomas, calretinin expression has been reported in adenomatoid tumors, which are rare benign mesothelial tumors that, even though they predominantly occur in the male and female genital organs, [69][70][71] may also arise in extragenital sites, including the pleura, 72,73 peritoneum, 74 intestinal mesentery, 75 omentum, 74 heart, 76 adrenal glands, 77,78 liver, 79,80 pancreas, 81 and mediastinum. 30,53 In contrast to epithelioid mesotheliomas, where it is generally accepted that calretinin is commonly expressed in the vast majority of cases (> 90%), some controversy exists in the literature regarding the frequency with which this marker is expressed in sarcomatoid mesotheliomas.…”

Section: Calretinin As a Mesothelial Markermentioning

confidence: 99%

Value of Calretinin Immunostaining in Diagnostic Pathology

Ordóñez

2014

Applied Immunohistochemistry &Amp; Molecular Morphology

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Calretinin is a member of the EF-hand family of calcium-binding proteins. Because its expression is highly restricted to mesotheliomas, calretinin is, at present, the most commonly used positive mesothelioma marker that is most often recommended to be included in the various immunohistochemical panels used to assist in the differential diagnosis of these tumors. Calretinin expression has also been reported to be commonly expressed in a wide variety of other neoplasms, including sex cord-stromal tumors, adrenal cortical neoplasms, olfactory neuroblastomas, Schwann cell tumors, cardiac myxomas, and ameloblastomas. This article reviews the information that is currently available on calretinin expression in tumors and on its application as an immunohistochemical marker in diagnostic pathology.

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Calretinin expression in tumors of adipose tissue

Cited by 14 publications

References 37 publications

Immunoreactivity for Calretinin and Keratins in Desmoid Fibromatosis and Other Myofibroblastic Tumors

Immunoreactivity for Calretinin and Keratins in Desmoid Fibromatosis and Other Myofibroblastic Tumors

Localization of peroxisome proliferator-activated receptor alpha (PPARÎ±) and N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) in cells expressing the Ca2+-binding proteins calbindin, calretinin, and parvalbumin in the adult rat hippocampus

Value of Calretinin Immunostaining in Diagnostic Pathology

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