Calretinin and Other CaBPs in the Nervous System

“…Because of their presence in the superficial dorsal horn, among different primary sensory afferents, it has been proposed that CBir and CRir cells serve regulatory roles in the processing of cutaneous and visceral sensory information [Philippe and Droz, 1988;Rogers et al, 1990;Ren et al, 1993;Ren and Ruda, 1994;Papka et al, 1999;Morona et al, 2006aMorona et al, , 2007. However, tracing studies to reveal the organization of the primary afferents to the spinal cord in lungfishes are needed to corroborate this suggestion.…”

Section: Dorsal Spinal Cordmentioning

confidence: 78%

Immunohistochemical Localization of Calbindin-D28k and Calretinin in the Spinal Cord of Lungfishes

Morona

González

2010

Brain Behav Evol

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A common pattern of distribution of neurons and fibers containing the calcium-binding proteins calbindin-D28k (CB) and calretinin (CR) in the spinal cord of terrestrial vertebrates has been recently demonstrated. Lungfishes are considered the closest living relatives of tetrapods, but practically no experimental data exist on the organization of their spinal cord. By means of immunohistochemical techniques, the localization of CB and CR was investigated in the spinal cord of the African (Protopterus dolloi) and Australian (Neoceratodus forsteri) lungfishes. Abundant cell bodies and fibers immunoreactive for either CB or CR were widely distributed throughout the spinal cord. A large population of immunoreactive cells was found in the dorsal column of the gray matter in both species, and abundant cells were distributed in the lateral and ventral columns. Ventrolateral motoneurons and multipolar cells were only intensely CB and CR immunoreactive in Neoceratodus. For the most part, separate cell populations contained either CB or CR, but a small subset of dorsally located neurons contained both in the two lungfishes. Colocalization was found in motoneurons and in ventrolaterally located cells only in Neoceratodus. Fiber labeling showed a predominance of CR-containing axons in the lateral and ventral funiculi of presumed supraspinal origin. These results show that lung-fishes and tetrapods have many features in common, suggesting that primitive anatomical, and likely functional, organization of the spinal cord of tetrapods is present in lungfishes.

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“…Many of the identified protein targets, including calretinin, cathepsin D, glyoxylase I, aldehyde dehydrogenase 2, and the calcium dependent protease Calpain 1, are associated with cancer development or RAS signaling. Calretinin, a mediator of intracellular calcium responses (Rogers et al, 1990), is a biomarker used in diagnoses of cancers, including epithelial ovarian tumors (Cao et al, 2001). Cathepsin D has been implicated in numerous studies as a cysteine protease involved in extracellular proteolysis of the basement membrane and metastasis to foreign tissue.…”

Section: Discussionmentioning

confidence: 99%

Proteomics analysis of H-RAS-mediated oncogenic transformation in a genetically defined human ovarian cancer model

et al. 2005

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RAS is a small GTP binding protein mutated in approximately 30% human cancer. Despite its important role in the initiation and progression of human cancer, the underlying mechanism of RAS-induced human epithelial transformation remains elusive. In this study, we probe the cellular and molecular mechanisms of RAS-mediated transformation, by profiling two human ovarian epithelial cell lines. One cell line was immortalized with SV40 T/t antigens and the human catalytic subunit of telomerase (T29), while the second cell line was transformed with an additional oncogenic ras V12 allele (T29H). In total, 32 proteins associated with RAS-mediated transformation have been identified using peptide mass fingerprinting. These protein targets are involved in several cellular pathways, including metabolism, redox balance, calcium signaling, apoptosis, and cellular methylation. One such target, the 40 kDa procaspase 4 is significantly upregulated at the protein level in RAS-transformed T29H cells, related directly to signaling through MEK, but not PI3 kinase. Cellular caspase 4 activity is, however, suppressed in the T29H cells, suggesting that the maturation process of caspase 4 is abrogated in RAS-transformed T29H cells. Consistent with this notion, transformed T29H cells were less susceptible to the toxic effects of anti-Fas antibody than were immortalized, nontransformed T29 cells, associated with less activation of caspase 4. This study demonstrates that functional proteomic analysis of a genetically defined cancer model provides a powerful approach toward systematically identifying cellular targets associated with oncogenic transformation.

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Calretinin and Other CaBPs in the Nervous System

Cited by 128 publications

References 18 publications

Immunohistochemical localization of recoverin-like Ca2+-binding protein (P23K) in mouse brain.

Immunohistochemical localization of recoverin-like Ca2+-binding protein (P23K) in mouse brain.

Immunohistochemical Localization of Calbindin-D28k and Calretinin in the Spinal Cord of Lungfishes

Proteomics analysis of H-RAS-mediated oncogenic transformation in a genetically defined human ovarian cancer model

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