CalR is required for the expression of T6SS2 and the adhesion of Vibrio parahaemolyticus to HeLa cells

Zhang, Lingyu; Osei‐Adjei, George; Zhang, Ying; Gao, He; Yang, Wenhui; Zhou, Dongsheng; Huang, Xinxiang; Yang, Huiying; Zhang, Yiquan

doi:10.1007/s00203-017-1361-6

Cited by 25 publications

(13 citation statements)

References 20 publications

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“…CalR is a LysR-type transcriptional regulator that was inhibited by the Calcium in V. parahaemolyticus [25]. CalR was originally identified as a repressor of the swarming motility, TDH2, and T3SS1, and previous studies have demonstrated that the biofilm formation, hemolytic activity and T6SS2 also could be regulated by CalR in V. parahaemolyticus [25,[33][34][35]. When the ∆VPA1701 strain was cultured in the BHI agar plates with 5 mM calcium, the swarming motility of ∆VPA1701 was recovered, but the swarming level was lower than the WT ( Figure 6A).…”

Section: Calcium Restored the Swarming Ability Of ∆Vpa1701mentioning

confidence: 99%

A GntR Family Transcription Factor (VPA1701) for Swarming Motility and Colonization of Vibrio parahaemolyticus

Meng

Yang

et al. 2019

Pathogens

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Motility is important for virulence, biofilm formation, and the environmental adaptation of many bacteria. Vibrio parahaemolyticus (V. parahaemolyticus) contains two flagellar systems that are responsible for motility, and are tightly regulated by transcription regulators and sigma factors. In this study, we identified a novel transcription factor, VPA1701, which regulates the swarming motility of V. parahaemolyticus. The VPA1701 deletion mutant (ΔVPA1701) eliminated the swarming motility on the surface of BHI agar plates and reduced colonization in infant rabbits. RNA-seq assays, confirmed by qRT-PCR, indicated that VPA1701 regulated the expression of lateral flagellar cluster genes. Further analyses revealed that VPA1701 directly binds to the promoter region of the flgBCDEFGHIJKL cluster to regulate the expression of lateral flagellar genes. CalR was originally identified as a repressor for the swarming motility of V. parahaemolyticus, and it was inhibited by calcium. In this study, we found that VPA1701 could inhibit the expression of the calR gene to increase the swarming motility of V. parahaemolyticus. Calcium downregulated the expression of calR, indicating that calcium could increase swarming motility of ΔVPA1701 by inhibiting calR. Thus, this study illustrates how the transcription factor VPA1701 regulates the expression of lateral flagellar genes and calR to control the swarming motility of V. parahaemolyticus.

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Section: Calcium Restored the Swarming Ability Of ∆Vpa1701mentioning

confidence: 99%

A GntR Family Transcription Factor (VPA1701) for Swarming Motility and Colonization of Vibrio parahaemolyticus

Meng

Yang

et al. 2019

Pathogens

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“…In V. cholerae , H-NS was shown to have many roles in silencing genes expression such as those required for capsule biosynthesis and virulence factors acquired by horizontal gene transfer (69-72). In this species, LeuO was shown to be required for biofilm formation, and LeuO and H-NS were shown to interact at the vieA locus (53, 68, 73, 74). In addition, in V. vulnificus another important human pathogen, ToxR was shown to activate leuO expression and LeuO was shown to be an auto-repressor with high levels of LeuO binding (73, 74).…”

Section: Discussionmentioning

confidence: 98%

“…Additionally, the global regulator ToxR was shown to be essential for LeuO expression (41). Also, in V. parahaemolyticus , LeuO was shown to be an antagonist of H-NS at loci required for type VI secretion system-1 (T6SS-1) expression (68). In V. cholerae , H-NS was shown to have many roles in silencing genes expression such as those required for capsule biosynthesis and virulence factors acquired by horizontal gene transfer (69-72).…”

Section: Discussionmentioning

confidence: 99%

NhaR, LeuO and H-NS are part of an expanded regulatory network for ectoine biosynthesis expression

Lichty

Gregory

Boyd

2022

Preprint

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Bacteria accumulate compatible solutes to maintain cellular turgor pressure when exposed to high salinity. In the marine halophile Vibrio parahaemolyticus, the compatible solute ectoine is biosynthesized de novo, which is energetically more costly than uptake; therefore, tight regulation is required. To uncover novel regulators of the ectoine biosynthesis ectABC-aspk operon, a DNA affinity pulldown of proteins interacting with the ectABC-aspk regulatory region was performed. Mass spectrometry analysis identified, amongst others, five regulators: LeuO, NhaR, OmpR, TorR, and the nucleoid associated protein H-NS. To determine their role in ectoine biosynthesis, in-frame non-polar deletions were made for each gene and PectA-gfp promoter reporter assays were performed. PectA-gfp expression was significantly repressed in the ΔleuO mutant and significantly induced in the ΔnhaR mutant compared to wild type, suggesting positive and negative regulation, respectively. Both ΔompR and ΔtorR mutants also showed induced expression of PectA-gfp, but not to the same extent as ΔnhaR. The Δhns showed no change compared to wild type. To examine whether H-NS interacts with LeuO or NhaR at the ectoine regulatory region, double deletion mutants were created. In a ΔleuO/Δhns mutant, PectA-gfp showed reduced expression, but significantly more than ΔleuO suggesting H-NS and LeuO interact to regulate ectoine expression. Whereas ΔnhaR/Δhns had no additional effect as compared to ΔnhaR suggesting NhaR regulation is independent of H-NS. Next, a PleuO-gfp reporter analysis was performed in wild type ΔleuO, Δhns, ΔleuO/Δhns and ΔtoxR, which encodes ToxR a positive regulator of leuO. PleuO-gfp showed significantly increased expression in the ΔleuO, Δhns and ΔleuO/Δhns mutants as compared to wild type, indicating both are repressors of leuO, and ΔtoxR showed reduced expression. Growth pattern analysis of the ΔleuO, Δhns, and ΔleuO/Δhns mutants in M9G 6%NaCl all showed growth defects compared to wild type, with ΔleuO/Δhns showing the greatest effect. Overall, the data show that NhaR is a negative regulator and LeuO is a positive regulator of ectoine expression and suggest LeuO is an anti-silencer of H-NS.

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“…This indicated that an unknown virulence factor could be responsible for pathogenicity. Other studies have suggested that the pathogenicity of this microorganism is probably also related to the Type 6 Secretion System (T6SS1 and T6SS2) (Li, Kinch, et al., 2017; Zhang, Gao, et al., 2017; Zhang, Osei‐Adjei, et al., 2017), adhesion factors (Jiang et al., 2014; Liu & Chen, 2015; Zhang, Osei‐Adjei, et al., 2017), iron‐uptake system (León‐Sicairos et al., 2015), lipopolysaccharide content (Guvener & McCarter, 2003; Zhang et al., 2018), proteases (Lee, Cheng, Yu, & Pan, 2002; Osei‐Adjei et al., 2017), and outer membrane proteins (Zha, Li, Li, Ye, & Pan, 2016). These studies have reported that the mechanism underlying the pathogenicity of V. parahaemolyticus and contributing factors is not completely understood.…”

Section: Distribution Of V Parahaemolyticus In Oystersmentioning

confidence: 99%

Managing the risk of Vibrio parahaemolyticus infections associated with oyster consumption: A review

Ndraha

Wong

Hsiao

2020

Comp Rev Food Sci Food Safe

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Vibrio parahaemolyticus is a Gram-negative bacterium that is naturally present in the marine environment. Oysters, which are water filter feeders, may accumulate this pathogen in their soft tissues, thus increasing the risk of V. parahaemolyticus infection among people who consume oysters. In this review, factors affecting V. parahaemolyticus accumulation in oysters, the route of the pathogen from primary production to consumption, and the potential effects of climate change were discussed.In addition, intervention strategies for reducing accumulation of V. parahaemolyticus in oysters were presented. A literature review revealed the following information relevant to the present study: (a) managing the safety of oysters (for human consumption) from primary production to consumption remains a challenge, (b) there are multiple factors that influence the concentration of V. parahaemolyticus in oysters from primary production to consumption, (c) climate change could possibly affect the safety of oysters, both directly and indirectly, placing public health at risk, (d) many intervention strategies have been developed to control and/or reduce the concentration of V. parahaemolyticus in oysters to acceptable levels, but most of them are mainly focused on the downstream steps of the oyster supply chain, and (c) although available regulation and/or guidelines governing the safety of oyster consumption are mostly available in developed countries, limited food safety information is available in developing countries. The information provided in this review may serve as an early warning for managing the future effects of climate change on the safety of oyster consumption. K E Y W O R D Sclimate change, food poisoning, oyster, risk, Vibrio parahaemolyticus Compr Rev Food Sci Food Saf.

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CalR is required for the expression of T6SS2 and the adhesion of Vibrio parahaemolyticus to HeLa cells

Cited by 25 publications

References 20 publications

A GntR Family Transcription Factor (VPA1701) for Swarming Motility and Colonization of Vibrio parahaemolyticus

A GntR Family Transcription Factor (VPA1701) for Swarming Motility and Colonization of Vibrio parahaemolyticus

NhaR, LeuO and H-NS are part of an expanded regulatory network for ectoine biosynthesis expression

Managing the risk of Vibrio parahaemolyticus infections associated with oyster consumption: A review

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