Calorimetry of microbial growth using a thermopile based microreactor

Higuera-Guisset, J.; Rodríguez‐Viejo, J.; Chacón, M.; Muñoz, Francisco; Vigués, Núria; Mas, Jordi

doi:10.1016/j.tca.2004.09.010

Cited by 54 publications

(35 citation statements)

References 17 publications

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“…Both types of calorimeters use a microfabricated membrane as their main structural component, and reaction chambers and thermometers are built on the membrane. Openchamber chip calorimeters have well structures as reaction chambers or simply use the bare membranes onto which [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43] In the case of the open-chamber chip calorimeters, the evaporation of the sample can significantly influence the measurements due to the lack of physical confinement. In fact, several early demonstrations of the operation of chip calorimeters showed large signals due to the evaporation of water droplets.…”

Section: Thermal Insulationmentioning

confidence: 99%

“…Early realization of the closed-chamber chip calorimeter was achieved by the simple assembly of open-chamber chip calorimeters with gaskets and tubings to form reaction chambers. 27,29,38,40,41 These large-scale tubings assembled with microscale chip calorimeters led to a large dead volume and a large heat loss. To reduce the sample consumption and to effectively measure the thermal event, the channel dimensions must be minimized and the channel must be localized within the sensing region.…”

Section: Fluid Handlingmentioning

confidence: 99%

See 1 more Smart Citation

Development and applications of chip calorimeters as novel biosensors

Lee¹,

Lee²,

Koh³

2012

NDD

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Calorimetry is the science of measuring heat. For more than 200 years now, calorimetry has advanced and various types of calorimeters have been developed for diverse applications. The development of microfabrication and microfluidics led to the advent of chip calorimeters, miniaturized calorimeters built and integrated as chips. Chip calorimeters, as labelfree biosensors, have many advantages due to their small sample volume and high-throughput capability. In this review, techniques for realizing chip calorimeters are discussed in terms of their major functional components: insulation, fluid handling, and thermometry. Recent trends in the development of chip calorimeters are also discussed, along with several application areas. New fabrication techniques can provide higher sensitivity and easier, more reliable sample handling for chip calorimeters, which would enable new application areas, such as the study of single cell metabolism.

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Section: Thermal Insulationmentioning

confidence: 99%

Section: Fluid Handlingmentioning

confidence: 99%

Development and applications of chip calorimeters as novel biosensors

Lee¹,

Lee²,

Koh³

2012

NDD

View full text Add to dashboard Cite

show abstract

“…Moreover, optimal time constants of nanocalorimeters permit fast control actions. Higuera-Guisset et al (2004) a nanocalorimeter working sensitively enough to be potentially suitable for the described control task. Arrays of chip calorimeters developed by Torres et al (2004) would even allow the control of a series of bioprocesses.…”

Section: Long-term Control Of the Continuous Conversion Of A Toxic Sumentioning

confidence: 99%

Control of continuous polyhydroxybutyrate synthesis using calorimetry and flow cytometry

Maskow

Müller

Lösche

et al. 2006

Biotech & Bioengineering

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The substrate-carbon flow can be controlled in continuous bioreactor cultures by the medium composition, for example, by the C/N ratio. The carbon distribution is optimal when a maximum fraction flows into the desired product and the residual is just sufficient to compensate for the dilution of the microbial catalyst. Undershooting of the latter condition is reflected immediately by changes in the Gibbs energy dissipation and cellular states. Two calorimetric measurement principles were applied to optimize the continuous synthesis of polyhydroxybutyrate (PHB) by Variovorax paradoxus DSM4065 during growth with constantly increasing supply rates of fructose or toxic phenol. Firstly, the changed slope of the heat production rate in a complete heat balanced bioreactor (CHB) indicated optimum carbon channeling into PHB. The extent of the alteration depended directly on the toxic properties of the substrate. Secondly, a flow through calorimeter was connected with the bioreactor as a "measurement loop." The optimum substrate carbon distribution was indicated by a sudden change in the heat production rate independent of substrate toxicity. The sudden change was explained mathematically and exploited for the long-term control of phenol conversion into PHB. LASER flow cytometry measurements distinguished between subpopulations with completely different PHB-content. Populations grown on fructose preserved a constant ratio of two subpopulations with double and quadruple sets of DNA. Cells grown on phenol comprised a third subpopulation with a single DNA set. Rising phenol concentrations caused this subpopulation to increase. It may thus be considered as an indicator of chemostress.

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“…This can pose problems in cases where only minute sample quantities are available for testing. There has been a strong demand to develop high throughput and high sensitivity calorimeter for rapid detection of biomolecular interaction for decades [16][17][18][19]. The microfabrication and nanofabrication technologies allow the miniaturization of the conventional bench-top scale instrument for the building of micro/nano DSC with microfluidic embedded systems, micro temperature sensors and heaters [20].…”

Section: Introductionmentioning

confidence: 99%

Review of MEMS differential scanning calorimetry for biomolecular study

Wang

et al. 2017

Front. Mech. Eng.

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Differential scanning calorimetry (DSC) is one of the few techniques that allow direct determination of enthalpy values for binding reactions and conformational transitions in biomolecules. It provides the thermodynamics information of the biomolecules which consists of Gibbs free energy, enthalpy and entropy in a straightforward manner that enables deep understanding of the structure function relationship in biomolecules such as the folding/unfolding of protein and DNA, and ligand bindings. This review provides an up to date overview of the applications of DSC in biomolecular study such as the bovine serum albumin denaturation study, the relationship between the melting point of lysozyme and the scanning rate. We also introduce the recent advances of the development of micro-electro-mechanic-system (MEMS) based DSCs.

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Calorimetry of microbial growth using a thermopile based microreactor

Cited by 54 publications

References 17 publications

Development and applications of chip calorimeters as novel biosensors

Development and applications of chip calorimeters as novel biosensors

Control of continuous polyhydroxybutyrate synthesis using calorimetry and flow cytometry

Review of MEMS differential scanning calorimetry for biomolecular study

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