Calorimetric Studies on Germinating and Quiescent Conidia of Actinomyces Streptomycini B-6

Kalakoutskii, L. V.; Pozharitskaja, L. M.

doi:10.2323/jgam.14.209

Cited by 5 publications

(1 citation statement)

References 6 publications

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“…Kalakoutskii and Bobkova (18) reported that L-valine stimulated germination of spores of Actinomyces streptomycini B-6 (Russian literature uses Actinomyces synonymously with Streptomyces; 20), whereas glutamic acid stimulated slightly and alanine had no effect. Shigaeva and Romankulov (25) tested the effect of 10 L-amino acids on germination of five Actinomyces (Streptomyces) species.…”

Section: Discussionmentioning

confidence: 99%

Nutritionally defined conditions for germination of Streptomyces viridochromogenes spores

Hirsch¹,

Ensign²

1976

J Bacteriol

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Spores of Streptomyces viridochromogenes were removed from the surface of solid media with glass beads and suspended in a buffer-detergent solution. Addition of yeast extract and glucose resulted in rapid loss of refractility of the spores. Appearance of germ tubes followed. Germination was accompanied by a decrease in the optical density (OD) of the suspension. The OD decrease was used as an assay for germination. A defined germination medium (DGM) comprised of L-alanine, L-glutamic acid, adenosine, para-aminobenzoic acid, and calcium and magnesium ions provided a germination rate nearly equal to that of complex media. The germination rate was essentially the same if i-alanine and D-glutamate replaced the L-isomers. The optimum pH and temperature for germination were 7.0 and 35 C. Germination was absolutely dependent on the presence of CO2. Spores harvested after growth for longer periods than the usual time (10 days) became less germinable in DGM. The same was observed for spores grown at 37 C as compared with 30 C. Spores incubated in DGM for various time periods before being transferred to a buffer solution did not continue to germinate. Spores harvested after growth of eight species of Streptomyces did not show a decrease in OD when incubated in yeast extract medium. Another strain of S. viridochromogenes did exhibit an OD decrease in the medium. Comparative properties of spores of streptomycetes, fungi, and bacilli are discussed.

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Section: Discussionmentioning

confidence: 99%

Nutritionally defined conditions for germination of Streptomyces viridochromogenes spores

Hirsch¹,

Ensign²

1976

J Bacteriol

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Mikroskopische Untersuchungen zur Wachstumskinetik von Streptomyces hygroscopicus

Schuhmann¹,

Bergter²

1976

J of Basic Microbiology

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Growth kinetics, branch formation, and cytological properties of mycelial growth of Streptomyces hygroscopicus on solid media were investigated by phase-contrast microscopy using a microculture method. Measurements were made on taken photographs of the growing hyphae from the beginning of spore germination up to maximal 18 hours. The specific growth rate of the germ tube was much higher than the specific growth rate of the mycelium. The doubling time of the total length of the mycelial hyphae and the doubling time of branch formation was quite the same for the period investigated. After a short time of outgrowth each individual hypha grows a t a constant rate, i.e. the length increases' linearly, but the growth kinetics of the whole mycelium becomes exponentially by branching. It seems, that nucleoids only divide in t h a t part of a hypha between the tip and the nearest branch. A cell unit (1.4-1.9 pm on different media) could be calculated by the length of a hypha and the number of nucleoids. A hypha is growing with the cell unit a t the tip or with the polar cap only. No interkalary growth in length could be found. Branches were formed only up to about 100 pm from the tip on complex medium and up to about 50 pm from the tip on mineral salt medium. A simple model of mycelial growth has been developed. Some properties showing the connection between Streptomycetes andprokaryoticorganisms on one hand and hyphal growing fungi on the other hand are discussed.

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Mikroskopische Untersuchungen zur Wachstumskinetik vonStreptomyces hygroscopicus

Schuhmann¹,

Bergter²

1976

Z Allg Mikrobiol

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Growth kinetics, branch formation, and cytological properties of mycelial growth of Streptomyces hygroscopicus on solid media were investigated by phase-contrast microscopy using a microculture method. Measurements were made on taken photographs of the growing hyphae from the beginning of spore germination up to maximal 18 hours. The specific growth rate of the germ tube was much higher than the specific growth rate of the mycelium. The doubling time of the total length of the mycelial hyphae and the doubling time of branch formation was quite the same for the period investigated. After a short time of outgrowth each individual hypha grows at a constant rate, i.e. the length increases linearly, but the growth kinetics of the whole mycelium becomes exponentially by branching. It seems, that nucleoids only divide in that part of a hypha between the tip and the nearest branch. A cell unit (1.4-1.9 mum on different media) could be calculated by the length of a hypha and the number of nucleoids. A hypha is growing with the cell unit at the tip or with the polar cap only. No interkalary growth in length could be found. Branches were formed only up to about 100 mum from the tip on complex medium and up to about 50 mum from the tip on mineral salt medium. A simple method of mycelial growth has been developed. Some properties showing the connection between Streptomycetes and prokaryotic organisms on one hand and hyphal growing fungi on the other hand are discussed.

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Calorimetric Studies on Germinating and Quiescent Conidia of Actinomyces Streptomycini B-6

Cited by 5 publications

References 6 publications

Nutritionally defined conditions for germination of Streptomyces viridochromogenes spores

Nutritionally defined conditions for germination of Streptomyces viridochromogenes spores

Mikroskopische Untersuchungen zur Wachstumskinetik von Streptomyces hygroscopicus

Mikroskopische Untersuchungen zur Wachstumskinetik vonStreptomyces hygroscopicus

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