Calorimetric methods for analysis of plant metabolism

Criddle, Richard S.; Hansen, Lee D.

doi:10.1016/s1573-4374(99)80016-8

Cited by 40 publications

(36 citation statements)

References 59 publications

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“…The branches' youngest leaves were removed with a razor blade, and approximately 100 mg of tissue placed in an ampule of an isothermal calorimeter (Hart Scientific Model 7707, Calorimetry Sciences Corporation Model 4100 or a similar 16-cell prototype calorimeter built by Calorimetry Sciences Corp., Lindon, Utah, USA). The metabolic heat rate (R q ) and rate of CO 2 production (R CO 2 ) were measured by the methods described in Criddle and Hansen (1999), Criddle et al (1997), and Hansen et al (2005). These measurements were repeated at 5 1C intervals, ranging downward from 20 to 10 1C (4 replicates), and upward from 20 to 30 1C (4 replicates) and 30 to 35 1C (10-12 replicates).…”

Section: Article In Pressmentioning

confidence: 99%

Two subspecies and a hybrid of big sagebrush: Comparison of respiration and growth characteristics

Hansen¹,

Farnsworth²,

Itoga³

et al. 2008

Journal of Arid Environments

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Section: Article In Pressmentioning

confidence: 99%

Two subspecies and a hybrid of big sagebrush: Comparison of respiration and growth characteristics

Hansen¹,

Farnsworth²,

Itoga³

et al. 2008

Journal of Arid Environments

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“…Specific rates of heat (R q ) and CO 2 R CO 2 ð Þ emission from shoot and leaf samples were determined by isothermal calorimetry at 20°C, following the well-established methods of Criddle et al (1990) and Criddle and Hansen (1999). Three multi-cell differential scanning calorimeters (model 4100, Calorimetry Sciences Corporation, UT, USA) were used simultaneously.…”

Section: Respiration Measurementsmentioning

confidence: 99%

“…Two such frameworks are the growth and maintenance paradigm (Amthor 2000; Cannell and Thornley 2000;Thornley and Cannell 2000) and the enthalpy balance model of respiration and growth (Hansen et al 1994;Criddle and Hansen 1999). According to the growth-maintenance paradigm, the observed positive correlations between growth and respiration rates can be explained by a respiration component, m, responsible for maintaining cellular function in existing biomass, and a second component, g, responsible for energizing growth.…”

Section: Introductionmentioning

confidence: 99%

Water stress impacts on respiratory rate, efficiency and substrates, in growing and mature foliage of Eucalyptus spp

Callister

Adams

2006

Planta

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In previous studies, water stress has induced variable and sometimes contradictory changes in respiration. We used isothermal calorimetry to measure the response of foliar respiration to water deficit in nine eucalypt genotypes. Specific growth rates (R(SG)) of shoots and leaves of variable age were measured independently, and the data were applied to both the growth-maintenance and enthalpy balance models. We calculated the oxidation state of respiratory substrate and the enthalpy change for the conversion of substrate carbon to biomass (DeltaH(B)). Moderate water stress reduced the R(SG) of shoots by 38% (P<0.01) and carbon conversion efficiency by 15% (P<0.05). The relationship between carbon conversion efficiency and R(SG) was not affected by water deficit for shoots, but was significantly altered for leaves. Water deficit increased maintenance respiration by about 23% (P<0.001). The growth coefficient of respiration was not significantly altered. However, changes in oxidation states of substrate and biomass suggest that the energy requirements of biosynthesis were increased under water stress. Our results confirm that carbohydrates are the major respiratory substrates in growing tissues, though mature leaves utilized a substantial component of more reduced substrate. Mature leaves had variable oxidation states for respiration substrate, which indicates a variable relationship between CO(2) evolution and ATP production. Measured DeltaH(B) in shoots and leaves were too small for reliable estimation of R(SG) by the enthalpy balance model. We also found significant effects of water stress on the oxidation state of substrate and DeltaH(B).

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“…In studies on plant physiology, calorimetry is an invaluable tool for investigating complex metabolic processes, including cell, tissue and organ responses to various environmental factors (Criddle and Hansen 1999). Calorimetric measurements of heat production have been used to estimate regeneration potential in oilseed rape callus ( _ Zur et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Rapid production of wheat cell suspension cultures directly from immature embryos

Biesaga‐Kościelniak

Kościelniak

Filek

et al. 2008

Plant Cell Tiss Organ Cult

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The aim of this study was to produce suspension cultures of winter wheat directly from immature embryos bypassing the callus stage, and to determine their capacity for growth and regeneration in comparison to suspension cultures produced from callus. The study was carried out using Polish winter wheat varieties: 'Grana' and 'Rosa'. Immature embryos were isolated, homogenized and transferred directly to liquid medium supplemented with 2,4-D. Actively dividing cell cultures were obtained within 2 months after the cultures were started. Suspension cultures from callus of immature embryos was also produced. With both cultivars, faster growth was observed in the suspension cultures produced directly from embryos than in the suspensions produced from callus. Metabolic activity was higher in the suspension culture produced directly from embryos than in the suspension derived from callus only in 'Grana'. The production of 1-amiocyclopropane-1-carboxylic acid (ACC), an ethylene precursor, was lower in the suspension cultures produced directly from embryos than in the suspensions produced from callus. Morphogenic capacity was significantly higher in aggregates derived directly from embryos than in aggregates derived from callus. With 'Rosa', about one third of the aggregates derived directly from embryos regenerated shoots. Production of ACC was lower in 'Rosa' cell culture that regenerated then in other cell cultures that did not. Photosystem II reactions were more efficient in dark green aggregates than in light green or pale green aggregates which were unable to regenerate. With the method presented, wheat cell suspension cultures with a regeneration potential can be produced in 2 or 3 months less time than with traditional methods.

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Calorimetric methods for analysis of plant metabolism

Cited by 40 publications

References 59 publications

Two subspecies and a hybrid of big sagebrush: Comparison of respiration and growth characteristics

Two subspecies and a hybrid of big sagebrush: Comparison of respiration and growth characteristics

Water stress impacts on respiratory rate, efficiency and substrates, in growing and mature foliage of Eucalyptus spp

Rapid production of wheat cell suspension cultures directly from immature embryos

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