Calorimetric investigation of aerobic fermentations

Birou, B.; Marison, I. W.; Stockar, Urs von

doi:10.1002/bit.260300509

Cited by 119 publications

(32 citation statements)

References 25 publications

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“…As a result, the ratio of heat production to oxygen consumption in all aerobic processes, whatever the strain involved and even if some amounts of fermentation products are formed, is always approximately given by 4Q 0 , i.e., 440 kJ/mol. This is in fair agreement with the observed average value of 450 kJ mol 1 [35]. This ratio, called sometimes the calo-respirometric coefficient, is the basis for the so-called "indirect calorimetry.…”

Section: Typical Heat Generation Curves During Microbial Growthsupporting

confidence: 88%

“…Even when r G 0 X was simply assumed to be approximately constant such as suggested by Figure 20 and a value of 500 kJ=C-mol was used in Eq. (35), the biomass yields were still predicted to about plus or minus 11% [62].…”

Section: Gibbs Energy Dissipation Correlationsmentioning

confidence: 94%

“…On the other hand, the heat yield per oxygen Y Q=O remains fairly constant, scattering [35]. The latter observation may be explained by the fact that the energy content of any organic compound in terms of its heat of combustion is directly proportional to the amount of O 2 consumed upon combustion.…”

Section: Typical Heat Generation Curves During Microbial Growthmentioning

confidence: 96%

“…A typical heat release curve obtained during the aerobic growth of a yeast culture is shown in Figure 6, [35]. As can be seen from the figure, the heat dissipation rate P q as a function of time may be integrated to obtain a curve showing the total heat (Q) generated up to a certain point in time, which parallels the biomass concentration x (g l 1 dry mass) quite nicely.…”

Section: Typical Heat Generation Curves During Microbial Growthmentioning

confidence: 98%

“…(36) and (37) were used in Eq. (35) in order to compare their ability to predict the biomass yields of a database containing 205 literature values including, among others, the whole database used earlier by Heijnen and van Dijken. It turned out that Eq.…”

Section: Gibbs Energy Dissipation Correlationsmentioning

confidence: 99%

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Biothermodynamics of live cells: a tool for biotechnology and biochemical engineering

Stockar

2010

Journal of Non-Equilibrium Thermodynamics

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The aim of this contribution is to review the application of thermodynamics to live cultures of microbial and other cells and to explore to what extent this may be put to practical use. A major focus is on energy dissipation effects in industrially relevant cultures, both in terms of heat and Gibbs energy dissipation. The experimental techniques for calorimetric measurements in live cultures are reviewed and their use for monitoring and control is discussed. A detailed analysis of the dissipation of Gibbs energy in chemotrophic growth shows that it reflects the entropy production by metabolic processes in the cells and thus also the driving force for growth and metabolism. By splitting metabolism conceptually up into catabolism and biosynthesis, it can be shown that this driving force decreases as the growth yield increases. This relationship is demonstrated by using experimental measurements on a variety of microbial strains. On the basis of these data, several literature correlations were tested as tools for biomass yield prediction. The prediction of other culture performance characteristics, including product yields for biorefinery planning, energy yields for biofuel manufacture, maximum growth rates, maintenance requirements, and threshold concentrations is also briefly reviewed.

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Section: Typical Heat Generation Curves During Microbial Growthsupporting

confidence: 88%

Section: Gibbs Energy Dissipation Correlationsmentioning

confidence: 94%

Section: Typical Heat Generation Curves During Microbial Growthmentioning

confidence: 96%

Section: Typical Heat Generation Curves During Microbial Growthmentioning

confidence: 98%

Section: Gibbs Energy Dissipation Correlationsmentioning

confidence: 99%

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Biothermodynamics of live cells: a tool for biotechnology and biochemical engineering

Stockar

2010

Journal of Non-Equilibrium Thermodynamics

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Continuous cultures limited by a gaseous substrate: Development of a simple, unstructured mathematical model and experimental verification with Methanobacterium thermoautotrophicum

Schill

Gulik

Voisard

et al. 2000

Biotechnol. Bioeng.

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This article presents a simple, unstructured mathematical model describing microbial growth in continuous culture limited by a gaseous substrate. The model predicts constant gas conversion rates and a decreasing biomass concentration with increasing dilution rate. It has been found that the parameters influencing growth are primarily the gas transfer rate and the dilution rate. Furthermore, it is shown that, for correct simulation of growth, the influence of gaseous substrate consumption on the effective gas flow through the system has to be taken into account. Continuous cultures of Methanobacterium thermoautotrophicum were performed at three different gassing rates. In addition to the measurement of the rates of biomass production, product formation, and substrate consumption, microbial heat dissipation was assessed using a reaction calorimeter. For the on‐line measurement of the concentration of the growth‐limiting substrate, H2, a specially developed probe has been used. Experimental data from continuous cultures were in good agreement with the model simulations. An increase in gassing rate enhanced gaseous substrate consumption and methane production rates. However, the biomass yield as well as the specific conversion rates remained constant, irrespective of the gassing rate. It was found that growth performance in continuous culture limited by a gaseous substrate is substantially different from “classic” continuous culture in which the limiting substrate is provided by the liquid feed. In this report, the differences between both continuous culture systems are discussed.

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