Calorimetric behaviour of individual phospholipid classes from human and bovine erythrocyte membranes

Dijck, P.W.M. Van; Zoelen, E.J.J. van; Seldenrijk, R.; Deenen, L.L.M. Van; Gier, J. De

doi:10.1016/0009-3084(76)90078-5

Cited by 53 publications

(13 citation statements)

References 18 publications

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“…However, an unequal distribution of cholesterol between the two monolayers of the membrane [46] and different cholesterol affinities for various phospholipids [47,48] have been demonstrated. Of the phospholipids in the erythrocyte membrane, phosphatidylethanolamine has the lowest affinity for cholesterol (van Dijck, P.W.M., unpublished results) and it undergoes a thermotropic transition from +10°C to --5°C [49]. It may be speculated that removal of the extrinsic proteins affects the association of cholesterol with the components in the inner monolayer of the membrane in such a manner that allows the phosphatidylethanolamine to undergo a liquid-crystalline to gel transition.…”

Section: Discussionmentioning

confidence: 99%

The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

Gerritsen

Verkleij

Deenen

1979

Biochimica et Biophysica Acta (BBA) - Biomembranes

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SummaryTriton X-100 (in concentrations which did not cause a significant solubilization of membrane material) caused aggregation of the intramembrane particles of human erythrocyte ghosts.Ghosts from which the extrinsic proteins had been removed by alkali treatment showed a temperature-induced aggregation of the particles. With virtually no spectrin present, the particles in these stripped ghosts could still be aggregated by manipulations with ionic strength and pH, or by the addition of calcium.Recombinant vesicles were made from a Triton X-100 extract and a mixture of phospholipids with a composition which resembled that of the inner monolayer of erythrocyte membrane. In these recombinants the same manipulations with ionic strength and pH and the addition of calcium caused a rearrangement of the particles, resulting in the appearance of particle-free areas. In recombinants prepared from a Triton X-100 extract and egg phosphatidylcholine the lateral distribution of the particles was not altered by these manipulations.It is concluded that in the erythrocyte membrane the intramembrane particles can be aggregated by effects of external agents on lipid components. In this light the role of spectrin in stabilizing the membrane by interactions with lipids in the inner monolayer is discussed.

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Section: Discussionmentioning

confidence: 99%

The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

Gerritsen

Verkleij

Deenen

1979

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…This contrasts with the gel-liquid crystalline transition, which is markedly broader in naturally occurring (see, for example, Ref. 46), as opposed to synthetic [47] lipid systems.…”

Section: Lipid Polymorphism: Model Systemsmentioning

confidence: 95%

“…Firstl gel-state lipids do not appear to be present in most biological membranes (particularly those of eukaryotic cells) as the unsaturated nature of most naturally occurring lipids results in hydrocarbon transitions which occur well below physiological temperatures (see, for example, data obtained for erythrocyte membrane lipids) [6,7]. Further in more metabolically active membranes (such as the inner mitochondrial and endoplasmic reticulum membranes [8,9]) where possible regulatory roles of lipids would be expected to be more obviously expressed, an increased rather than a decreased unsaturation of the tipids is observed.…”

Section: Ia Functional Roles Of Lipids In the Fluid Mosaic Model Of mentioning

confidence: 99%

Lipid polymorphism and the functional roles of lipids in biological membranes

Cullis

Kruijff

1979

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

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853

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“…The leaflet selectivity of each of the probes (i.e., transbilayer distribution) within isolated plasma membrane fragments was verified by leaflet-selective quenching studies performed as reviewed in Refs. 35, 36 and detailed in the following individually cited papers showing that the inner and outer leaflets of isolated plasma membrane vesicles/fragments differ in fluidity: i) leaflet-selective quenching of DPH (nonselectively partitions into both leaflets) (37)(38)(39); ii) studies with right side-out and inside-out plasma membranes (38); iii) studies with the leaflet-selective DPH derivatives DPH-TMA and DPH-Pro (39), which are anchored closer to the membrane surface than DPH (40); iv) spin-label probes and purified right side-out and inside-out oriented plasma membranes (41); and v) model membrane studies of fluidity of outer versus inner leaflet phospholipid mixtures (42). Based on the these previous findings, fluorescence polarization of the leaflet-selective DPH derivatives DPH-TMA and DPH-Pro were used to measure the transbilayer fluidity gradient in hepatocyte plasma membrane lipid rafts and nonrafts exactly as described earlier (13,39).…”

Section: Structure Of Hepatocyte Plasma Membrane Lipid Raft and Nonramentioning

confidence: 99%

SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes

Atshaves¹,

McIntosh²,

Payne³

et al. 2007

Journal of Lipid Research

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Although reverse cholesterol transport from peripheral cell types is mediated through plasma membrane microdomains termed lipid rafts, almost nothing is known regarding the existence, protein/lipid composition, or structure of these putative domains in liver hepatocytes, cells responsible for the net removal of cholesterol from the body. Lipid rafts purified from hepatocyte plasma membranes by a nondetergent affinity chromatography method were: i) present at 33 6 3% of total plasma membrane protein; ii) enriched in key proteins of the reverse cholesterol pathway [scavenger receptor class B type I (SR-B1), ABCA1, P-glycoprotein (P-gp), sterol carrier protein-2 (SCP-2)]; iii) devoid of caveolin-1; iv) enriched in cholesterol, sphingomyelin, GM1, and phospholipids low in polyunsaturated fatty acid and double bond index; and v) exhibited an intermediate liquid-ordered lipid phase with significant transbilayer fluidity gradient. Ablation of the gene encoding SCP-2 significantly altered lipid rafts to: i) increase the proportion of lipid rafts present, thereby increasing raft total content of ABCA1, P-gp, and SR-B1; ii) increase total phospholipids while decreasing GM1 in lipid rafts; iii) decrease the fluidity of lipid rafts, consistent with the increased intermediate liquid-ordered phase; and iv) abolish the lipid raft transbilayer fluidity gradient. Thus, despite the absence of caveolin-1 in liver hepatocytes, lipid rafts represented nearly one-third of the mouse hepatocyte plasma membrane proteins and displayed unique protein, lipid, and biophysical properties that were differentially regulated by SCP-2 expression. Although all cells synthesize cholesterol, only liver and steroidogenic tissues effect net cholesterol removal. Reverse cholesterol transport (RCT) from peripheral tissues to liver for oxidation and excretion is the major physiological pathway for the net cholesterol elimination from the body. Although increasing evidence indicates that plasma membrane microdomains, termed lipid rafts/caveolae, compartmentalize cell surface proteins that mediate HDLcholesterol uptake/efflux in peripheral cells, the existence and/or significance of such domains in hepatocyte plasma membranes is not completely clear, especially in view of the nearly negligible levels of caveolin-1 in liver. However, an overall scheme is beginning to evolve based largely on findings with peripheral cells (reviewed in Ref. 1).First, peripheral cells (fibroblasts, muscle, and endothelial cells) are rich in both lipid rafts and caveolae (a subset of lipid rafts rich in several components of the RCT pathway) but are relatively poor in intracellular cholesterol binding proteins such as sterol carrier protein-2 (SCP-2) that facilitate cholesterol retention rather than efflux (reviewed in Refs. 1, 2). Cholesterol effluxes from peripheral cells to HDL by a process facilitated by: i) the HDL receptor scavenger receptor class B type I (SR-B1) (3); ii) ABCA1, a protein that mediates cholesterol and phosphatidylcholine desorption from the pla...

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Calorimetric behaviour of individual phospholipid classes from human and bovine erythrocyte membranes

Cited by 53 publications

References 18 publications

The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

Lipid polymorphism and the functional roles of lipids in biological membranes

SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes

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