Calorie restriction intervention induces enterotype-associated BMI loss in nonobese individuals

Zou, Huajie; Wang, Dan; Ren, Huahui; Chen, Peishan; Fang, Chao; Shi, Zhun; Zhang, Pengfan; Wang, Jian; Yang, Huanming; Cai, Kaiye; Huang, Zhong

doi:10.1101/514596

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…1, Supplementary Table S4). Principal coordinate analysis (PCoA) as well as Dirichlet multinominal mixture (DMM) model using Bray-Curtis dissimilarity showed that the microbiomes of this Chinese cohort could be represented by two clusters dominated by Bacteroides and Prevotella 15 , containing 440 and 178 individuals, respectively (Fig. 1a).…”

Section: Characteristics Not Reported In European Cohortsmentioning

confidence: 99%

A genome-wide association study for gut metagenome in Chinese adults illuminates complex diseases

et al. 2021

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The gut microbiome has been established as a key environmental factor to health. Genetic influences on the gut microbiome have been reported, yet, doubts remain as to the significance of genetic associations. Here, we provide shotgun data for whole genome and whole metagenome from a Chinese cohort, identifying no <20% genetic contribution to the gut microbiota. Using common variants-, rare variants-, and copy number variations-based association analyses, we identified abundant signals associated with the gut microbiome especially in metabolic, neurological, and immunological functions. The controversial concept of enterotypes may have a genetic attribute, with the top two loci explaining 11% of the Prevotella–Bacteroides variances. Stratification according to gender led to the identification of differential associations in males and females. Our two-stage metagenome genome-wide association studies on a total of 1295 individuals unequivocally illustrates that neither microbiome nor GWAS studies could overlook one another in our quest for a better understanding of human health and diseases.

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Section: Characteristics Not Reported In European Cohortsmentioning

confidence: 99%

A genome-wide association study for gut metagenome in Chinese adults illuminates complex diseases

et al. 2021

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“…1, Table S1D ), possibly due to higher prevalence of Prevotella in developing countries (Dhakan et al, 2019; Vangay et al, 2018). Principle coordinate analysis (PCoA) as well as Dirichlet multinominal mixture model using Bray-Curtis dissimilarity showed that this Chinese cohort could be formed into two clusters dominated by Bacteroides and Prevotella (Zou et al, 2019), containing 440 and 178 individuals, respectively (Fig. 1A).…”

Section: Resultsmentioning

confidence: 99%

M-GWAS for the gut microbiome in Chinese adults illuminates on complex diseases

Liu

Tang

Huang

et al. 2019

Preprint

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SummaryThe gut microbiome has been established as a key environmental factor to health. Genetic influences on the gut microbiome have been reported, yet, doubts remain as to the significance of genetic associations. Here, we provide shotgun data for whole genome and whole metagenome from a Chinese cohort, identifying no less than 20% genetic contribution to the gut microbiota. Using common variants-, rare variants- and copy number variations (CNVs)-based association analyses, we identified abundant signals associated with the gut microbiome especially in metabolic, neurological and immunological functions. The controversial concept of enterotypes may have a genetic attribute, with the top 2 loci explaining 11% of the Prevotella-Bacteroides variances. Stratification according to gender led to the identification of differential associations in males and females. Genetically encoded responses to ectopic presence of oral bacteria in the gut appear to be a common theme in a number of diseases investigated by MWAS (Metagenome-wide association studies). Our two-stage M-GWAS (Microbiome genome-wide association studies) on a total of 1295 individuals unequivocally illustrates that neither microbiome nor GWAS studies could overlook one another in our quest for a better understanding of human health and diseases.HighlightsM-GWAS using high-depth whole genome identifies contributions from rare variants and CNVs.Gut microbial modules such as butyrate, amino acids, mucin degradation show genetic associations.Gender differential M-GWAS underscores differences in metabolic and psychological predispositions.Some of the MWAS markers for colorectal cancer and cardiometabolic diseases show genetic associations.

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“…Calorie restriction is a nutrition intervention to reduce energy intake. In a long-term observational study of calorie restriction, it was found that calorie restriction can reduce body weight and the risk of chronic disease in people who are not obese (Zou et al, 2019). Calorie restriction plays a role in creating a balance and diversity of the gut microbiota ecosystem (Wang et al, 2018), preventing excess energy from causing systemic inflammation that occurs when adipose tissue expands to accommodate extra nutrient storage (Ye and Keller, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of Calorie Restriction on Intestinal Inflammation in Mice Treated with a High-Calorie Diet

Harlinda¹,

Wiyasihati²,

Kurniasari³

et al. 2022

CMUJNS

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Inflammatory bowel disease (IBD) is classified into ulcerative colitis and Crohn's disease. It is chronic inflammation of gastrointestinal tract and idiopathic disease. Sugar is proved to be risk factor for IBD. Calorie restriction is a nutrition intervention to reduce energy intake and can reduce chronic desease. This study aims to determine the difference in the effect of a high-calorie diet and calorie restriction on intestinal inflammation. Eight-week-old healthy female Balb/c mice were divided into two groups. The high calorie (HC) group was fed standard diet plus oral gavage of glucose solution every day for 4 weeks. The dose of glucose solution was 0.0325 ml/gBW or 3-5% additional calories each day. The calorie restriction (CR) group had their calorie intake restricted to 50% of standard diet 3 times a week. After 4 weeks of treatment, the mice had their colon removed. The assessment of the intestinal inflammation was carried out using a light microscope, with a scoring system by Erben et al (2014) in HE staining. The mean score of the HC group was 1.22, this was higher than the CR group with a mean score of 1.18 even tough the result revealed that the scores of intestinal inflammation were not significantly different in the two groups (P = 0.827). However, there is no evidence for the underlying mechanism of intestinal inflammation by high-calorie diet and improvement of intestinal inflammation in high-calorie diet via calorie restriction. Thus, further research is needed on other calorie restriction methods in high-calorie diet study. Keywords: High calorie diet, Calorie restriction, Intestinal inflammation

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Calorie restriction intervention induces enterotype-associated BMI loss in nonobese individuals

Cited by 4 publications

References 40 publications

A genome-wide association study for gut metagenome in Chinese adults illuminates complex diseases

A genome-wide association study for gut metagenome in Chinese adults illuminates complex diseases

M-GWAS for the gut microbiome in Chinese adults illuminates on complex diseases

Effect of Calorie Restriction on Intestinal Inflammation in Mice Treated with a High-Calorie Diet

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