Caloric Restriction Increases the Expression of Heat Shock Protein in the Gut

Ehrenfried, J. A.; Evers, B. Mark; Chu, Kyo U.; Townsend, Courtney M.; Thompson, James D.

doi:10.1097/00000658-199605000-00015

Cited by 55 publications

(30 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reporter-CR has previously been reported to induce hsp70 expression (33,34). To verify that our CR conditions induce a HSR in C. elegans, we visualized GFP expression, which is under the regulatory control of the hsp70 promoter (C12C8.1::GFP) by microscopy (26).…”

Section: Cr and Hs Synergize To Activate The Hsp70 Promotermentioning

confidence: 95%

Heat Shock and Caloric Restriction Have a Synergistic Effect on the Heat Shock Response in a sir2.1-dependent Manner in Caenorhabditis elegans

Raynes

Leckey

Nguyen

et al. 2012

Journal of Biological Chemistry

View full text Add to dashboard Cite

Section: Cr and Hs Synergize To Activate The Hsp70 Promotermentioning

confidence: 95%

Heat Shock and Caloric Restriction Have a Synergistic Effect on the Heat Shock Response in a sir2.1-dependent Manner in Caenorhabditis elegans

Raynes

Leckey

Nguyen

et al. 2012

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…HSPs were named for the observation that heat exposure induces their synthesis (Schlesinger 1990). However, their abundance at mRNA and protein levels, particularly the highly inducible HSP70, is upregulated by many stressors, including those that cause oxidative stress (Wheeler et al 1995;Ehrenfried et al 1996;Gasbarrini et al 1998).…”

Section: Introductionmentioning

confidence: 99%

HSP70 Abundance and Antioxidant Capacity in Feeding and Fasting Gray Seal Pups: Suckling Is Associated with Higher Levels of Key Cellular Defenses

Bennett¹,

MacMillan

Hammill

et al. 2014

Physiological and Biochemical Zoology

View full text Add to dashboard Cite

Heat shock proteins (HSPs) and antioxidants are key cellular defenses against stress. Seals routinely undergo protracted fasting, which is normally associated with physiological stress in other animals. We tested the hypotheses that (1) relative HSP70 protein abundance is higher in liver and blubber of fasting relative to suckling wild gray seal pups; (2) differences in HSP70 are mirrored in tissue superoxide dismutase (SOD) and catalase activity, as well as glutathione levels; (3) extracellular HSP70 correlates with hepatic and blubber HSP70 abundance; and (4) protein carbonylation, an index of oxidative damage, is lower in tissues with higher levels of these cellular stress markers. In contrast to our expectation, suckling pups had higher relative HSP70 abundance and glutathione levels in liver and blubber and higher hepatic catalase activity. Plasma HSP70 did not correlate with liver or blubber abundance of the protein. Suckling pups did not experience greater protein carbonylation, suggesting that cellular protective mechanisms prevent protein damage despite an apparent increase in cellular stress. SOD activity was not affected by nutritional state, but in blubber tissue, it was positively correlated with blubber thickness. Greater requirements for antioxidants and HSPs in suckling pups or in animals with thicker blubber could arise from rapid protein synthesis, high metabolic fuel availability, and/or exposure to lipophilic toxins. Developmental and nutritional changes in cellular defenses have important implications for gray seals' susceptibility to additional stress exposure.

show abstract

“…Several endogenous physiological or environmental stressors, such as heat, ethanol, heavy metals, caloric restriction, and bacterial infection can trigger gut epithelial cells to increase synthesis of heat shock proteins (HSPs) (7,27,31,40,54,58). However, certain HSPs are expressed under nonstress conditions and play an important role in maintaining cell integrity (20,21,30,43).…”

mentioning

confidence: 99%

Luminal bacterial flora determines physiological expression of intestinal epithelial cytoprotective heat shock proteins 25 and 72

Arvans¹,

Vavricka²,

Ren³

et al. 2005

American Journal of Physiology-Gastrointestinal and Liver Physi

107

View full text Add to dashboard Cite

and 72 are expressed normally by surface colonocytes but not by small intestinal enterocytes. We hypothesized that luminal commensal microflora maintain the observed colonocyte HSP expression. The ability of the small intestine to respond to bacteria and their products and modulate HSPs has not been determined. The effects of luminal bacterial flora in surgically created midjejunal self-filling (SFL) vs. self-emptying (SEL) small-bowel blind loops on epithelial HSP expression were studied. HSP25 and HSP72 expression were assessed by immunoblot and immunohistochemistry. SFL were chronically colonized, whereas SEL contained levels of bacteria normal for the proximal small intestine. SFL creation significantly increased HSP25 and HSP72 expression relative to corresponding sections from SEL. Metronidazole treatment, which primarily affects anaerobic bacteria as well as a diet lacking fermentable fiber, significantly decreased SFL HSP expression. Small bowel incubation with butyrate ex vivo induced a sustained and significant upregulation of HSP25 and altered HSP72 expression, confirming the role of short-chain fatty acids. To determine whether HSPs induction altered responses to an injury, effects of the oxidant, monochloramine, on epithelial resistance and short-circuit current (I sc) responses to carbachol and glucose were compared. Increased SFL HSP expression was associated with protection against oxidant-induced decreases in transmural resistance and I sc responses to glucose, but not secretory responses to carbachol. In conclusion, luminal microflora and their metabolic byproducts direct expression of HSPs in gut epithelial cells, an effect that contributes to preservation of epithelial cell viability under conditions of stress.

show abstract

Caloric Restriction Increases the Expression of Heat Shock Protein in the Gut

Cited by 55 publications

References 25 publications

Heat Shock and Caloric Restriction Have a Synergistic Effect on the Heat Shock Response in a sir2.1-dependent Manner in Caenorhabditis elegans

Heat Shock and Caloric Restriction Have a Synergistic Effect on the Heat Shock Response in a sir2.1-dependent Manner in Caenorhabditis elegans

HSP70 Abundance and Antioxidant Capacity in Feeding and Fasting Gray Seal Pups: Suckling Is Associated with Higher Levels of Key Cellular Defenses

Luminal bacterial flora determines physiological expression of intestinal epithelial cytoprotective heat shock proteins 25 and 72

Contact Info

Product

Resources

About