Calorespirometry in Plant Biology

Plant Cell & Environment

Florez‐Sarasa

et al. 2009

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We tested the hypotheses that relative activity of the less efficient alternative oxidase (AOX) path changes with diurnal temperature changes, and thus changes carbon use efficiency with temperature. The activities of the alternative and cytochrome oxidase (COX) paths in plant tissues of three species were determined by measuring 18 O/ 16 O discrimination and total respiration from 17 to 36°C. A new, more accurate method for calculating oxygen uptake rate from the mass spectrometry data was developed. Total carbon use efficiency was calculated from the ratio of respiratory heat and CO2 rates measured from 10 to 35°C. Oxygen isotope discrimination (22.9 Ϯ 0.4‰) and AOX participation were invariant with temperature in leaf tissue of Cucurbita pepo, Nicotiana sativa and Vicia faba, thus falsifying the first part of the hypothesis. Stress responses of respiration at the temperature extremes limited the range for which carbon use efficiency could be accurately measured to 15-30°C in N. sativa, to 10-25°C in C. pepo and to 20-30°C in V. faba. Carbon-use efficiency was invariant at these temperatures in these species, demonstrating that changes in other pathways that would vary carbon-use efficiency were also invariant with temperature.

Section: Methodsmentioning

confidence: 99%

“…where Δ H O2 is Thornton's constant (470 kJ mol −1 O 2 for carbohydrates) and Δ H B is the difference in the heats of combustion of carbohydrate and leaf tissue (Δ H B was assumed equal to 30 kJ mol −1 for the purposes of this study) (Macfarlane, Adams & Hansen 2002; Hansen et al. 2005; Macfarlane et al.…”

Section: Methodsmentioning

confidence: 99%

Plant mitochondria electron partitioning is independent of short‐term temperature changes

Macfarlane

Plant Cell & Environment

Florez‐Sarasa

et al. 2009

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“…see Gifford 2003, Taylor et al 1998, van Iersel and Seymour 2000, van Iersel 2003, Yamaguchi 1978) and indirect measurement through measurements of R CO2 and the oxygen uptake rate, R O2 , or equivalently, the respiratory heat production rate, R q (e.g. see Criddle and Hansen 1999, Hansen et al 2002, 2005, Macfarlane et al 2002). Note that heat and oxygen rates are directly proportional as stated by Thornton's rule (Battley 1999, Hansen et al 2004): …”

Section: Methods For Measuring Substrate Carbon Conversion Efficiencymentioning

confidence: 99%

“…common garden growth studies can only pick the best grower at the location and temperature pattern at the garden location. Calorespirometric determination of the growth curve vs temperature (Criddle et al 1997, Criddle and Hansen 1999, Hansen et al 2005) enables matching the growth curve to a local temperature pattern (i.e. the curve of time‐at‐temperature vs temperature) without growing the plant at the actual location and temperatures it is adapted to.…”

Section: Temperature Dependence Of Growth Ratesmentioning

confidence: 99%

“…The calorespirometric method for the determination of ε is based on the following relations (Criddle and Hansen 1999, Hansen et al 2002, 2004, 2005, Macfarlane et al 2002). The metabolic heat rate is the sum of the heat rates from the two reactions (anabolism and catabolism) comprising the above overall reaction 1: …”

Section: Calorespirometric Measurement Of Substrate Carbon Conversionmentioning

confidence: 99%

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Temperature responses of substrate carbon conversion efficiencies and growth rates of plant tissues

Thomas

Arnholdt‐Schmitt

2009

Physiologia Plantarum

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Growth rates of plant tissues depend on both the respiration rate and the efficiency with which carbon is incorporated into new structural biomass. Calorespirometric measurement of respiratory heat and CO 2 rates, from which both efficiency and growth rate can be calculated, is a well established method for determining the effects of rapid temperature changes on the respiratory and growth properties of plant tissues. The effect of the alternative oxidase/cytochrome oxidase activity ratio on efficiency is calculated from first principles. Data on the temperature dependence of the substrate carbon conversion efficiency are tabulated. These data show that ε is maximum and approximately constant through the optimum growth temperature range and decreases rapidly as temperatures approach temperature limits to growth. The width of the maximum and the slopes of decreasing ε at high and low temperatures vary greatly with species, cultivars and accessions.

Calorespirometry as a tool for studying temperature response in carrot (Daucus carota L.)

Nogales

Muñoz-Sanhueza

Engineering in Life Sciences

et al. 2013

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Calorespirometric measurements of metabolic heat rates and CO2 emission rates of respiring tissues as functions of temperature enable rapid determination of the temperatures that plants are adapted to without growing them in different environmental temperatures. However, the correct choice of target material for measurements that enable prediction of growth temperature responses is crucial, and needs to be identified in a species‐ and trait‐specific manner. In this study, different carrot materials were tested: a primary culture system proposed as an in vitro test system for carrot yield potential, taproots of young plants, and the root meristem of actively growing plants during secondary root growth. The central root meristem is the most suitable for studying temperature response by calorespirometry for genotype comparison. Calorespirometric methods for predicting genotype‐specific temperature responses of crop plant cultivars can be used to predict productivity in environments with differing temperature conditions.