Calmodulin-dependent cyclic nucleotide phosphodiesterase (PDE1) splice variants from bovine cardiac muscle

Das, Shankar B.; Dinh, Chris; Shah, Sachin; Olson, Doug; Ross, Andrew R. S.; Selvakumar, Ponniah; Sharma, Rajendra K.

doi:10.1016/j.gene.2007.03.012

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…Compound 2, the main constituent, was the most potent PDE5 and PDE6 inhibitor, but less than sildenafil ( Table 2). Even though PDE5/PDE6 selectivities were poor, they showed high selectivity over PDE1, which is abundant in the heart [19]. This appears to be the first study reporting phenanthrenes inhibiting PDEs and pharmacologically-active constituents of E. macrobulbon.…”

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confidence: 77%

Phenanthrenes from Eulophia macrobulbon as Novel Phosphodiesterase-5 Inhibitors

Temkitthawon

Changwichit

Khorana

et al. 2017

Natural Product Communications

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Phosphodiesterase 5 (PDE5) inhibitors can be used for the treatment of erectile dysfunction and pulmonary hypertension. In order to search for new leads of PDE5 inhibitors, we investigated the chemical constituents of the tubers of Eulophia macrobulbon (E.C. Parish & Rchb. f.) Hook. f. A new phenanthrene, 9,10-dihydro-4-(4-hydroxybenzyl)-2,5-dimethoxyphenanthrene-1,7-diol (1) and three known phenanthrenes i.e., 1-(4-hydroxybenzyl)-4,8dimethoxyphenanthrene-2,7-diol (2), (9,10-dihydro-2,5-dimethoxyphenanthrene-1,7-diol (3) and 1,5,7-trimethoxyphenanthrene-2,6-diol) (4) were isolated. Among these, 2 was the most potent PDE5 inhibitor (IC 50 =1.67±0.54 µM) evaluated by the [ 3 H]cGMP radioassay method, whereas 1 showed mild activity (IC 50 = 62.3±3.3 µM). Their inhibitory selectivities against PDE5 over PDE6 were also studied. This study suggests phenanthrenes as a new class of PDE5 inhibitors.

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confidence: 77%

Phenanthrenes from Eulophia macrobulbon as Novel Phosphodiesterase-5 Inhibitors

Temkitthawon

Changwichit

Khorana

et al. 2017

Natural Product Communications

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“…Amongst eukaryotes enzymes like cyclic nucleotides phosphodiesterase (PDE)12, plasma membrane ATPases13, myosin light chain kinase14 and NAD kinase15 have been shown to be calmodulin-dependent. In prokaryotes calcium mediated signaling has been shown to regulate various processes like cell structure, motility, cell division, gene expression and cell differentiation processes such as sporulation, heterocyst formation and fruiting body development16.…”

Section: Discussionmentioning

confidence: 99%

Calmodulin-like protein from M. tuberculosis H37Rv is required during infection

Advani

Rajagopalan

Reddy

2014

Sci Rep

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M. tuberculosis constitutes very sophisticated signaling systems that convert the environment signals into appropriate cellular response and helps the bacilli to overcome the onslaught of host defence mechanisms. Although mycobacterial two-component systems and STPKs have gained lot of attention as virulence factors, mycobacterial calcium signaling has not been very well studied. Calcium signaling has been the primary mechanism in eukaryotes for regulation of kinases, however in prokaryotes auto-phosphorylation of number of kinases has been reported. We have previously reported a small calmodulin-like-protein (CAMLP) from M. tuberculosis regulating enzymes of heterogeneous origin. To understand its role in both viability and virulence, we have assessed the effect of reduced expression of CAMLP coding gene Rv1211 on M. tb growth in vitro and ex vivo. Further, we have also studied the expression profile of Rv1211 in various conditions simulating host microenvironments. Our results highlight the possible role of CAMLP in growth and survival of M. tb during infection.

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