1990
DOI: 10.1042/bj2660261
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Calmodulin dependence of transferrin receptor recycling in rat reticulocytes

Abstract: Kinetic analysis of transferrin receptor properties in 6-8 day rat reticulocytes showed the existence of a single class of high-affinity receptors (Kd 3-10 nM), of which 20-25% were located at the cell surface and the remainder within an intracellular pool. Total transferrin receptor cycling time was 3.9 min. These studies examined the effects of various inhibitors on receptor-mediated transferrin iron delivery in order to define critical steps and events necessary to maintain the functional integrity of the p… Show more

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Cited by 35 publications
(20 citation statements)
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“…7), indicating that Caz+ influx contributes to Tf-R recycling. This result was in agreement with those in previous papers (Grasso et al, 1990;Morimoto et al, 1995). The similar action of perhexiline, EGTA, BAPTA and Ni2+ on the recycling process may be explained by their interference with Ca".…”
Section: Discussionsupporting
confidence: 93%
See 1 more Smart Citation
“…7), indicating that Caz+ influx contributes to Tf-R recycling. This result was in agreement with those in previous papers (Grasso et al, 1990;Morimoto et al, 1995). The similar action of perhexiline, EGTA, BAPTA and Ni2+ on the recycling process may be explained by their interference with Ca".…”
Section: Discussionsupporting
confidence: 93%
“…Different steps in the endocytosis pathway involve interactions between cytosolic Caz+-binding proteins and membranes. For example, a calmodulin-dependent Tf-R recycling rate was demonstrated in K562 cells (Hunt and Marshall-Carlson, 1986), reticulocytes (Grasso et al, 1990) and MDCK cells (Apodaca et al, 1994). Similarly, calmodulin is involved in the exocytosis process in chromaffin cells (Chamberlain et al, 1995) and in unicellular organisms (Kerboeuf et al, 1993).…”
mentioning
confidence: 99%
“…Staurosporine (10 nM), a C-kinase inhibitor (25) with Ki of 0.7 nM for C-kinase (per Kamiya Biochem Co.) completely blocked the effect of PMA (500 nM). Calmidazolium, a calmodulin inhibitor with IC50 in the micromolar range (26), was used to examine the importance of the Ca2`-calmodulin pathway. Staurosporine (10 nM) or calmidazolium (1 MM) either alone or together had no effect on GSH synthesis but pretreatment with either agent partially blocked the effect of phenylephrine (0.1 mM).…”
Section: Resultsmentioning
confidence: 99%
“…Perhaps one reason is that ehrlichial internalization is dependent on a calcium-dependent enzyme, TGase, and microtubules (40). A second reason is that to accommodate proliferating ehrlichial organisms, new membranes should be added and iron and other nutrients should be supplied by fusion of ehrlichial inclusions with transferrin receptor endosomes (7), which are Ca 2ϩ -calmodulin-and TGase-dependent processes (19,23). A striking finding of the present study was that PLC-␥2 and tyrosine-phosphorylated proteins were rapidly recruited and retained with ehrlichial inclusions even 2 days p.i.…”
Section: Discussionmentioning
confidence: 99%