Calmin expression in embryos and the adult brain, and its regulation by all‐trans retinoic acid

Abstract

The vitamin A metabolite, all-trans retinoic acid (atRA), is a regulator of nervous system development. Using a subtracted cDNA library constructed from neuroblastoma cells, the atRA-responsive gene calmin (Clmn) was identified (Merrill et al. [2004] Biol Chem 385:605-614). The Clmn transcript is detected very early in rat embryonic development and is sensitive to retinoid status. In vitamin A-deficient embryos, Clmn mRNA is dramatically down-regulated in the neuroepithelium adjacent to the somites, and this expression can be rescued with the addition of atRA. In embryonic day 18.5 embryos, CLMN is detected in regions where newly differentiated neurons are found, including the neural retina and the cortical plate; and in the adult brain, CLMN is most highly expressed in the neuron cell bodies of the hippocampus, cerebellum, and olfactory bulb. Thus, Clmn is sensitive to retinoid status during early gestational stages, and its expression is relegated to postmitotic neuronal cells in the adult rat brain. Developmental Dynamics 239:610-619,

Neuronal Migration Defect of the Developing Cerebellar Vermis in Substrains of C57BL/6 Mice: Cytoarchitecture and Prevalence of Molecular Layer Heterotopia

“…These searches revealed tissue hybridized for 56 different genes. From this dataset we identified MLH in tissue hybridized for an additional 9 genes with previously reported expression in PCs including: Bai3 [32], Cacna2d2 [33], Clmn [34], Cst3 [35], ERp29 [36], Grid2 [37], Hpca [38], Id2 [39], and Nefh [40]. As was seen for Calb1 and Cacna1g (online suppl.…”

Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration

“…CLMN was first identified in mouse embryonic dorsal epidermis as a vertebrate-specific calponin homology (CH) and transmembrane (TM) domain-containing protein localizing to intracellular membranes 22 (Figure 1E). Further studies confirmed CLMN expression in a variety of tissues, including the mouse testis, liver, kidney, large intestine, and in developing and mature mouse brain in a manner sensitive to all-trans retinoic acid 22,[33][34][35][36] . Structurally, the CH domains of CLMN are most closely related to those of the LINC complex components Nesprin 1/2 37 (Figure 1E) and could represent an actin-binding module for CLMN, as CH domains are known to interact directly with actin 38 .…”

Identification of Genomic Regions and Candidate Genes for Litter Traits in French Large White Pigs Using Genome-Wide Association Studies

“…In addition, knockout of DICER1 in mouse causes morphologic abnormalities and stunted growth and lethality [ 38 ]. Mark et al reported that the CLMN gene can affect the development of nervous system in early embryos [ 39 ]. There were two genes on SSC 6, WWOX and UBR4 .…”