Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium

Marsolais, A. A.; Kasha, K. J.

doi:10.1139/b85-313

Cited by 29 publications

(10 citation statements)

References 3 publications

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“…For all liquid media with Ficoll, the protocol was similar to the one of Marsolais & Kasha (1985), where 3ml of induction medium were put into each Falcon Petri dish (60 × 15 mm). After each 10-day period, 1 ml of medium was removed and replaced by 1 ml of fresh replenishment medium in which the sugar dose was decreased to 3% and IAA was replaced by 2,4-D (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Comparison of media for their aptitude in wheat anther culture

Trottier

Collin

Comeau

1993

Plant Cell Tiss Organ Cult

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Different media were evaluated with anthers of five spring wheat (Triticum aestivum L.) genotypes for their ability to produce embryos and green plants in anther culture. Our first experiment showed that the addition of a combination of 19 amino acids significantly increased the number of embryos and green plants obtained. The mean number of green plants per 100 anthers for the two genotypes in this experiment, HY320 and B723, went from 28.2 without amino acids in the medium, to 46.7 with addition of amino acids. Our second experiment with the genotypes HY320, Wim and Laval-19 showed that liquid medium with Ficoll is more efficient for anther culture (9.9 green plants/100 anthers) than solid (0 green plants), gelatinous media (2.5 green plants/100 anthers) or liquid medium with Membrane Rafts (0 green plants; Hoechst Celanese Corp.). Our third experiment revealed that the effect of replacement of sucrose by maltose varied with the genotype of the donor plant. Maltose partially inhibited the androgenesis of three responsive genotypes, HY320, Wim and Reliance (40.3 green plants/100 anthers instead of 43.9 with sucrose), while maltose significantly increased the androgenesis of the recalcitrant genotype Laval-19 (10.8 green plants/100 anthers instead of 5.4 with sucrose). An amino acid x maltose interaction was also observed. Amino acids without maltose increased androgenesis, but the addition of maltose to the amino acid-enriched medium eliminated this positive effect of the amino acids.

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Section: Methodsmentioning

confidence: 99%

Comparison of media for their aptitude in wheat anther culture

Trottier

Collin

Comeau

1993

Plant Cell Tiss Organ Cult

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“…Controversy exists over the possible beneficial effects of low temperature pre-treatment on barley anther culture response and some workers [3,9] recommend that anthers should be removed from spikes and cultured directly. The present factorial experiment was therefore designed to investigate the effects of pre-treatment and culture system on anther culture response in barley cultivars which are of importance in current breeding programmes.…”

Section: Introductionmentioning

confidence: 99%

The influence of genotype and temperature pre-treatment on anther culture response in barley (Hordeum vulgare L.)

Powell¹

1988

Plant Cell Tiss Organ Cult

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A~tract. The influence of temperature stress pre-treatment on anther culture response has been examined in eight commercially desirable barley cultivars. Spikes were pre-treated in darkness at 4°C for periods of 0, 7, 14, 21 and 28 days. Overall, the optimum pre-treatment period was 21 days, although there were large genotype by pre-treatment interactions. The most responsive cultivar was Igri, with a mean of 38% anthers responding, and relatively little effect of pre-treatment. The greatest effect of pre-treatment was in cv. Heriot, which had 3% response with no pre-treatment and 52% response from 14 days pre-treatment.

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“…1). The frequency of responsive tTCL explants increased as the concentration of TDZ ranged from 0.5 to 1.0 mg/L Although addition of 2,4-D has been found to elicit rapid cell proliferation and callus formation, reduction or removal of 2,4-D from the medium is essential for plant regeneration from calli (Marsolais and Kasha, 1985;Liang et al, 1987;Ball et al, 1993). New gene product synthesized upon the removal of 2,4-D were required for the transition from globular stage to the heart-shaped stage (Zimmerman, 1993).…”

Section: Resultsmentioning

confidence: 99%

High Frequency Plant Regeneration System from Transverse Thin Cell Layer Section of <i>In vitro</i> Derived ‘Nadia’ Ginger Microrhizome

Thingbaijam

HUIDROM

2014

Not Sci Biol

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An efficient and reproducible procedure is outlined for rapid in vitro multiplication of Zingiber officinale var. 'Nadia' through high frequency shoot proliferation from transverse thin cell layer (tTCL) sections of in vitro derived microrhizome. In vitro derived microrhizome of size 500 µm in thickness was used as initial explants for induction of somatic embryos. Among the different phytohormones tested, tTCL explants shows maximum calli proliferation in medium containing 2 mg/L 2,4-Dichlorophenoxyacetic acid (88.30±0.11%). Reduced concentration of 2,4-Dichlorophenoxyacetic acid was supplemented with different cytokinins for regeneration of callus. Among the different medium tested, optimum redifferentiation of somatic embryos were observed in medium containing 0.2 mg/L 2,4-Dichlorophenoxyacetic acid and 6.0 mg/L BAP (141.08±0.25). Clump of regenerated plantlets were further subculture and transfer into microrhizome inducing medium containing high sucrose concentration (8%). Plantlets with well developed microrhizome were successfully acclimatized and eventually transferred to the field. The application of studying embryo section for regeneration of plants might be useful alternative to ginger improvement programme. Histological analysis showed formation of somatic embryos and regenerated adventitious shoot.

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Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium

Cited by 29 publications

References 3 publications

Comparison of media for their aptitude in wheat anther culture

Comparison of media for their aptitude in wheat anther culture

The influence of genotype and temperature pre-treatment on anther culture response in barley (Hordeum vulgare L.)

High Frequency Plant Regeneration System from Transverse Thin Cell Layer Section of <i>In vitro</i> Derived ‘Nadia’ Ginger Microrhizome

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