Callus Induction and Somatic Embryogenesis from Leaf and Nodal Explants of Lycium barbarum L. (Goji)

Osman, Nafisah; Awal, Abdul; Sidik, Norrizah Jaafar; Abdullah, Shamsiah

doi:10.3923/biotech.2013.36.45

Cited by 18 publications

(21 citation statements)

References 9 publications

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“…All these findings are in agreement with the findings of present work as also a combination of 2,4-D + Kinetin gave better result with respect to callus induction in leaf explants when cultured in MS medium along with the above plant growth regulators. Osman et al, (2013) used MS + 1.0 mg/l 2,4-D + 0.3 mg/l BAP and found 100% callus induction in the leaf explants of Lycium barbatum L., an important herb used in traditional Chinese medicine. Kalsaitkar et al, (2014) induced callus in leaf explants of Bacopa monnieri in MS + 2,4-D + Kinetin, at different concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Tissue culture studies for micropropagation and callus regeneration have been done by Arumugam and Bhojwani (1990), Rani and Grover (1999), Martin (2004), Tsay Hsin-Sheng and Agarwal, (2005), Wood Ward and Bartel (2005), Sakakibara (2006), Bahadur et al, (2007), Chaturvedi et al, (2007), Monga et al, (2008, Wang et al, (2008), Siva et al, (2009), Dash and Mandal (2010), Joshi et al, (2010), Juan et al, (2010), Showkat et al, (2010), Ahmad et al, (2011), Madhulkar et al, (2011), Rao et al, (2011), Rout et al, (2011), Subashri and Kolipillai (2012), Jayakumar and Lingam (2013), Kalsaitkar et al, (2014), Kaur et al, (2013), Osman et al, (2013), Sundriyal et al, (2013), Kumari Ritika et al, (2014, Subhashri and Pillai (2014), Thangavel et al, (2014), Anita (2017), Aradkani et al, (2017), Abiri et al, (2017), Khattak et al, (2017), Rajeev et al, (2018) and Srivastva et al, (2017). In the present work impact of plant growth regulators & adjuvant on the growth of callus have been studied.…”

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confidence: 99%

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Induction Of Callus From Different Explants Of Bacopa monnieri And Effect Of Adjuvant On The Growth Rate Of The Calli

Kumari¹

2019

Indian Journal of Scientific Research

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Present work was carried out to observe the impact of growth regulators the auxin and cytokinin to observe their combined impact on callus induction and these calli were subcultured in the presence of adjuvant, here coconut milk to observe its impact on the growth rate of the calli. During the work it was noted that all the explants such as young leaf, mature leaf, and the internodes responded to different concentrations of 2,4-D and Kinetin, but highest percentage of response for swelling and callus induction was noted in the mature leaf explants when it was inoculated in MS + 1.5 mg/l 2,4-D + 0.4 mg/l Kinetin that was 94.68 and 90.34 respectively. This was followed by the internodal explants in the same medium and the percentage of response was 88.72 for swelling the and 78.50 for callus induction. Here minimum response was in young leaf explants for swelling 78.25 and for callusing 73.46 respectively. Similar trends of response were noted in case of all the explants cultured in the medium supplemented with 1.0 mg/l 2,4-D + 3 mg/l Kinetin. Callus raised from mature leaf in MS + 1.5 mg/l 2,4-D + 0.4 mg/l Kinetin, was subcultured in the same medium with coconut milk as adjuvant, in the ratio of 0.5%, 1.0% and 1.5% which was 5ml/l, 10ml/l and 15ml/l. After three weeks the growth rate & biomass were calculated. It was observed that MS + 1.5 mg/l 2,4-D + 0.4 mg/l Kinetin and 1.5% coconut milk promoted better growth as was noted from the diameter and fresh weight of the calli in subculture. Here, the growth response, growth rate and weight of the calli were lowest in the medium containing 0.5% coconut milk.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Induction Of Callus From Different Explants Of Bacopa monnieri And Effect Of Adjuvant On The Growth Rate Of The Calli

Kumari¹

2019

Indian Journal of Scientific Research

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“…In vitro regeneration of techniques has been popular methods to conserve the endangered species. Callus formation is one of the important aspects in plant biotechnology and considered to be among the most important subject in micropropagation technologies [18]. Callus resulted from culturing of explants on callus induction media will produce regeneration of plant through the process of organogenesis or somatic embryogenesis [5].…”

Section: Introductionmentioning

confidence: 99%

Embryogenic Callus Induction of Aquilaira Malaccensis Lam . a nd Aquilaria Subintegra Ding Hou

Salam*¹,

Awal²,

Abdullah³

2019

IJEAT

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Aquilaria malaccensis Lam. and Aquilaria subintegra Ding Hou belong to the family of Thymelaeaceae which is commonly known as gaharu or agarwood. It is a commercially important tree and identified as a potential aromatic plant. The overwhelming responses in the lodging sector reduce gaharu species in the forest. Mass propagation through plant tissue culture technology will substitute this problem. The present study was conducted to investigate the embryogenic callus induction between these two species. The most optimum sterilization method for both species was sodium hypochlorite 5.0% which gave the highest percentage of aseptic culture (95%) with the absence of tissue browning. The leaves of both species were cultured on Murashige and Skoog, (1962) (MS) media supplemented with combination of various concentrations of 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 2.5 mg/L) and 2,4-dichlorophenoxyacetic acid (2, 4-D) (0.5, 1.0, 1.5 and 2.0 mg/L) and kept under dark condition. The explants produced embryogenic, white and compact callus at the end cut of the explants after two weeks of culture in all treatments. The highest frequency of embryogenic callus formation was observed in explants cultured on 2.0 mg/L BAP and 0.5 mg/L 2,4-D for both species. From the present study, the optimum sterilization technique and embryogenic callus induction for A. malaccensis Lam. and A. subintegra were established.

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“…In vitro cultures is one of the methods used for the rapid multiplication of many plant species, regardless of weather conditions (Osman et al 2013;Tarinejad 2013;Krupa-Małkiewicz and Ochmian 2014;Teixeira da Silva et al 2016). However, the success of micropropagation is determined by many factors.…”

Section: Introductionmentioning

confidence: 99%

The Effects Nano-Silver on Contamination of Spring Barley ‘Eunova’ in Vitro

Krupa-Małkiewicz¹,

Ochmian²,

MIJOWSKA³

et al. 2017

Folia Pomer. Univ. Technol. Stetin., Agric., Aliment., Pisc., Z

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Streszczenie. Jedną z nowych metod dezynfekcji materiału roślinnego w kulturach in vitro jest zastosowanie noanosrebra. Związek ten nie wykazuje toksycznego działania na roślinę, natomiast cechuje się wysoką skutecznością w eliminowaniu zakażeń bakteryjnych, wirusowych czy grzybowych. W pracy zbadano wpływ różnych stężeń i sposobów aplikacji nanosrebra na skuteczność dezynfekcji, cechy morfologiczne (wysokość i długość korzeni) oraz barwę liści jęczmienia jarego 'Eunova' w kulturach in vitro. Materiałem wyjściowym doświadczenia były zarodki jęczmienia, które wykładano na pożywkę MS uzupełnioną 4.0 i 6.0 mg • dm-3 nanosrebra oraz zarodki moczone wcześniej w roztworze nanosrebra 50 i 100 mg • dm-3 w czasie 2 i 4 h. Zarodki jęczmienia moczone 70-procentowym alkoholem etylowym stanowiły próbę kontrolną. Po 28 dniach doświadczenia zaobserwowano, że zarówno sposób dezynfekcji, jak i czas działania oraz stężenie roztworu nanosrebra miały wpływ na liczbę zakażeń, długość pędu i korzeni oraz barwę liści siewek jęczmienia. Najmniej skuteczne (100% zakażeń) okazało się moczenie zarodków w 50 mg • dm-3 roztworu nanosrebra przez 2 h. Natomiast najlepszą skuteczność dezynfekcji (brak zakażeń) uzyskano po dodaniu do pożywki MS 4.0 mg • dm-3 nanosrebra. Podobną skuteczność dezynfekcji uzyskano, mocząc zarodki w 100 mg • dm-3 roztworu nanosrebra oraz wykładając zarodki na pożywkę MS uzupełnioną nanosrebrem, niezależnie od jego stężenia.

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Callus Induction and Somatic Embryogenesis from Leaf and Nodal Explants of Lycium barbarum L. (Goji)

Cited by 18 publications

References 9 publications

Induction Of Callus From Different Explants Of Bacopa monnieri And Effect Of Adjuvant On The Growth Rate Of The Calli

Induction Of Callus From Different Explants Of Bacopa monnieri And Effect Of Adjuvant On The Growth Rate Of The Calli

Embryogenic Callus Induction of Aquilaira Malaccensis Lam . a nd Aquilaria Subintegra Ding Hou

The Effects Nano-Silver on Contamination of Spring Barley ‘Eunova’ in Vitro

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