Callus induction and somatic embryogenesis of Musa spp. ABB cv. Pisang Awak

Sipen, Philip; Anthony, Paul; Davey, M. R.

doi:10.1016/j.cbpa.2008.04.540

Cited by 1 publication

(1 citation statement)

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“…Plant regeneration via somatic embryogenesis in bananas has been reported from embryogenic cell suspensions established from embryogenic callus induced from apical meristems (Cronauer & Krikorian 1985), corm-like tissues (Novak et al 1989;Navarro, Escobedo, & Mayo 1997), pseudostems, leaf bases and rhizome fragments (Novak et al 1989), highly proliferating scalps (Dhed'a et al 1991;Schoofs 1997;Ganapathi et al 2001b;Sipen, Anthony, & Davey 2008), immature zygotic embryos (Escalant & Teisson 1989;Marroquin et al 1993), immature male flowers (Ma 1991;Shii et al 1992;Grapin et al 1998;Chung et al 2006;Sidha et al 2007;Jalil et al 2008), and immature female flowers (Navarro, Escobedo, & Mayo 1997;Grapin et al 2000). In general, embryogenic cell suspensions of banana Downloaded by [198.91.36.79] at 07:21 18 February 2015 are usually established from immature male flowers and scalps (Strosse et al 2003).…”

Section: Micropropagationmentioning

confidence: 97%

Genetic Improvement of Banana Using Conventional andIn VitroTechnologies

Sipen

Chubo

King

et al. 2011

Journal of Crop Improvement

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Banana ( Musa spp.) is an important nutrient-rich fruit crop cultivated in the tropics and sub-tropics for local consumption and export. Targets for genetic improvement of banana range from improved fruit quality, yield, disease resistance, tolerance to biotic and abiotic stresses, and the biosynthesis of pharmaceutical compounds. Sterility has limited the success of generating new cultivars by conventional breeding. Tissue culture-based technologies that involve embryo rescue, the generation of somaclonal variation, and gene-transfer procedures are a useful adjunct to sexual hybridization, although considerable effort is required to establish robust protoplast-to-plant systems for somatic hybridization. Transformation involving Agrobacterium and biolisticsmediated gene transfer is feasible, underpinned by shoot regeneration from cultured cells and tissues. Molecular characterization of germplasm will facilitate the selection of material most relevant for incorporation into sexual and somatic genetic-improvement programs.

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