Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant

Noor, Wasifa; Lone, Rafiq; Kamili, Azra N.; Husaini, Amjad M.

doi:10.1016/j.btre.2022.e00762

Cited by 9 publications

(5 citation statements)

References 39 publications

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“…Shoots develop before root initiation. Previous studies reported the highest regeneration rates in rice on MS medium supplemented with BA, kinetin and TDZ [30][31][32] . Here, BA, kinetin and TDZ were tested at various concentrations to assess shoot, leaf and root regeneration from calli produced on a semi-solid MS medium without 1-naphthylacetic acid (NAA) supplementation because 2-3 mg LG 1 of NAA could inhibit cytokinin accumulation and suppress growth and morphogenesis 33 .…”

Section: Discussionmentioning

confidence: 89%

Influence of 2,4-Dichlorophenoxyacetic Acid on Callus Induction and Synthetic Cytokinins on Plant Regeneration of Three Phetchaburi Indigenous Rice Varieties

Ayudhay¹,

Kanjanawar²,

Sridaphan³

et al. 2022

Asian J. of Crop Science

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Background and Objective: Rice is one of the most important global foods. In Thailand, numerous indigenous rice varieties have many valuable traits improved by biotechnology. This study optimized concentrations of 2,4-D for callus induction and three synthetic cytokinins for plant regeneration. Materials and Methods: Efficient callus induction and in vitro regeneration were developed using plant growth regulators on three selected Thai indigenous rice varieties from Phetchaburi Province (Rai-Ma-Kham, RK, Ta-Pleum, TP and Kon-Chud, KC). Callus induction frequency (CIF) and callus growth were performed using four supplemented concentrations (0, 2, 4 and 6 mg LG 1 ) of 2,4-D (2, 4-Dichlorophenoxyacetic Acid). Plant regeneration using three synthetic cytokinins [6-benzyl amino purine, BA, kinetin and thidiazuron (TDZ)] was also performed in terms of the number of shoots, leaves and roots at concentrations 0.1, 0.5, 1.0 and 2.0 mg LG 1 to maximize induction of regenerable calli. Results: All three rice varieties revealed 100% frequency of callus induction on semi-solid MS (Murashige and Skoog) medium at all 2,4-D supplemented levels. Regeneration of shoots and leaves from BA was found in TP and KC rice, while the RK variety did not respond. Root regeneration numbers varied significantly in both the supplemented medium and the control MS medium. The suitable cytokinin concentration for RK rice was 0.1 mg LG 1 of kinetin, TP rice was 1.0 mg LG 1 of kinetin, while for KC rice a combination of 0.1 mg LG 1 TDZ and 0.1 mg LG 1 BA was suggested. Conclusion: The 2,4-D was an ideal callus-induced regulator, while different concentrations of the three synthetic cytokinins were selected to manipulate each rice variety.

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Section: Discussionmentioning

confidence: 89%

Influence of 2,4-Dichlorophenoxyacetic Acid on Callus Induction and Synthetic Cytokinins on Plant Regeneration of Three Phetchaburi Indigenous Rice Varieties

Ayudhay¹,

Kanjanawar²,

Sridaphan³

et al. 2022

Asian J. of Crop Science

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“…Finally, we found RM-Id was the optimum regeneration media comprising of CHU(N6) basal, 5 mg/L BAP, 0.02mg/L NAA along with sorbitol as desiccant carbohydrate, and sucrose as carbon source. Though 5 mg/L BAP concentration in RM-I media is reported to be optimum(Noor et al 2022), no report is found where as low as 0.02 mg/L of NAA is used in RM-I before our study. During second phase of regeneration, either MS or CHU(N6) is reported as basal media in RM-II for rice regeneration(Paramasivam & Ann, 2020).…”

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confidence: 74%

Low Titre of Agroinoculum with Prolonged Incubation Period and Low Auxin Concentration in the Regeneration Media are the Key to High Frequency of Transformation in Climate Resilient Aus type Rice Genotype Nagina 22

Kumar,

Priyanka,

et al. 2024

Preprint

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Developing a highly efficient and reproducible plant regeneration protocol holds paramount significance in advancing technology for the genetic transformation of rice genotypes, facilitating their further utilization in crop improvement and breeding programs. Nagina 22 (N22), an aus type rice genotype, has tolerance against multiple stresses, requires very efficient transformation and regeneration protocol, for developing improved lines. However, a standardized regeneration protocol for this elite genotype has not been reported so far, limiting the use of this genotype as a background for genetic transformation. In the present study, for the first time, we are reporting a highly efficient transformation and regeneration protocol for the climate resilient N22. Mature seeds were used as explants for callus induction with varying concentrations (1-4 mg/L) of 2,4-dichlorophenoxyacetic acid (2.4-D), among which 3 mg/L of 2,4-D resulted in an impressive ~94% callus induction efficiency. Following this, the regeneration and resuspension media optimization is also carried out. Subsequent steps involved culturing embryogenic calli on media with different concentrations (2-6 mg/L) of 6-benzylaminopurine (BAP), in combination with 0.02 mg/L naphthalene acetic acid (NAA), to optimize the regeneration protocol. Media consisting of 5 mg/L BAP and 0.02 mg/L NAA exhibited a regeneration frequency of ~92% for the plantlets derived through non-transformed callus. Furthermore, our study revealed that reducing the salt concentration in the resuspension media resulted a notable enhancement in transformation efficiency of 44%. This in-vitro regeneration protocol provides valuable insights for future research and practical applications in genetic transformation of this elite rice genotype for various purposes.

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“…In vitro regeneration is an effective technique for producing disease-free and genetically accurate plants [ 14 ]. However, as numerous factors, such as the type of explants, combination of plant growth regulators on media, culturing parameters, genotype, etc., influence the in vitro regeneration of plants, their biochemical properties may differ from those of in vivo materials [ 29 , 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of antibacterial activity and cytotoxic effects of in vitro and in vivo plant parts of a medicinal plant Gynura procumbens (Lour.) Merr.

Saha,

Al Amin,

Khan

et al. 2023

Heliyon

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Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant

Cited by 9 publications

References 39 publications

Influence of 2,4-Dichlorophenoxyacetic Acid on Callus Induction and Synthetic Cytokinins on Plant Regeneration of Three Phetchaburi Indigenous Rice Varieties

Influence of 2,4-Dichlorophenoxyacetic Acid on Callus Induction and Synthetic Cytokinins on Plant Regeneration of Three Phetchaburi Indigenous Rice Varieties

Low Titre of Agroinoculum with Prolonged Incubation Period and Low Auxin Concentration in the Regeneration Media are the Key to High Frequency of Transformation in Climate Resilient Aus type Rice Genotype Nagina 22

Assessment of antibacterial activity and cytotoxic effects of in vitro and in vivo plant parts of a medicinal plant Gynura procumbens (Lour.) Merr.

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