Callus Induction and Plant Regeneration from Mature Embryos of a Diverse Set of Wheat Genotypes

Zale, Janice; Borchardt-Wier, Harmony; Kidwell, K. K.; Steber, Camille M.

doi:10.1023/b:ticu.0000009248.32457.4c

Cited by 69 publications

(60 citation statements)

References 8 publications

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“…Explants of T. monococcum formed callus readily (92.2-97.8%), but their embryogenic capacity (0.7-2.8%) and plant regeneration efficiency (0.7-1.7%) were extremely low. This observation is in agreement with previous reports that showed either nonembryogenic callus formation or only occasional plant regeneration from cultured explants of T. monococcum (Lazar et al 1983, Zale et al 2004, Yin et al 2011.…”

Section: Resultssupporting

confidence: 83%

“…Proliferation was induced in somatic cell cultures of tetraploid timopheevii wheat (AAGG genome) and hexaploid spelt wheat (AABBDD genome), although no shoot differentiation was observed (Lazar et al 1983) or the number of regenerated plants was low (Yang et al 2015(Yang et al , Özgen et al 2015. Some attempts to establish reliable plant regeneration protocols using various tissues of diploid wheat species (AA genome) failed to produce positive results (Lazar et al 1983, Zale et al 2004, Bi et al 2007, Yin et al 2011). According to Yang et al (2015), plantlet regeneration by means of somatic embryogenesis represents a great obstacle for various germplasms, e.g., T. carthlicum, T. macha and T. polonicum.…”

Section: о Alikina Et Almentioning

confidence: 99%

“…Wheat includes more than 20 cultivated species (Goncharov 2011), however substantial research effort has been dedicated mainly to durum and bread wheat, owing to their prevalence in the world production (Fennel et al 1996, Machii et al 1998, Barro et al 1999, Bohorova et al 2001, He and Lazzeri 2001, Zale et al 2004, Vendruscolo et al 2008, Yin et al 2011. Until recently, many wheat species were not analyzed for plant regeneration ability in vitro; but nowadays, the interest in such studies is on the rise (Chang et al 2012, Yang et al 2015, Özgen et al 2015.…”

Section: о Alikina Et Almentioning

confidence: 99%

“…Efficient in vitro culture of mature wheat embryos was established by two main techniques. Embryos should be isolated from mature in the same way as from immature seeds, followed by placing the entire (Chauhan et al 2007(Chauhan et al , Özgen et al 2015, or longitudinally bisected (Zale et al 2004, Yu et al 2008 or multiple divided embryos (Delporte et al 2014) on callus induction medium. Alternatively, in vitro plant regeneration under endosperm-supported culture may be achieved by placing the whole seed with the wounded mature embryo on induction medium (Özgen et al 1998(Özgen et al , Filippov et al 2006).…”

Section: о Alikina Et Almentioning

confidence: 99%

See 3 more Smart Citations

Tissue culture efficiency of wheat species with different genomic formulas

Alikina

Chernobrovkina

Dolgov

et al. 2016

Crop Breed. Appl. Biotechnol.

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Section: Resultssupporting

confidence: 83%

Section: о Alikina Et Almentioning

confidence: 99%

Section: о Alikina Et Almentioning

confidence: 99%

Section: о Alikina Et Almentioning

confidence: 99%

See 2 more Smart Citations

Tissue culture efficiency of wheat species with different genomic formulas

Alikina

Chernobrovkina

Dolgov

et al. 2016

Crop Breed. Appl. Biotechnol.

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“…(Kim et al, 2003). Resultados similares também foram alcançados por Zale et al (2004), os quais observaram que os embriões zigóticos maduros de cultivares de Triticum monococcum L. e T. tauschii (Coss.) Schmalh.…”

Section: Resultsunclassified

Indução de embriões somáticos a partir de embriões zigóticos de Heliconia bihai (L.) L. cv. Lobster Claw Two

et al. 2011

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Somatic embryogenesis from zygotic embryos of Heloconia bihai (L.) L. cv. Lobster Claw TwoTropical flowers have contributed to the growth of the cut flower market in Brazil, especially plants of the genus Heliconia. Heliconias are usually propagated vegetatively, although this method can spread diseases and in the near future may severely comprise commercial flower production. In vitro cultivation represents an important potential alternative for guaranteeing the production of high-quality heliconia planting stocks. However, the micropropagation of these plants using stalk apices faces severe problems of endophytic contamination, making it difficult the supply of enough explants. Therefore, alternative techniques for the in vitro production of planting stock were investigated using other explant tissues to induce somatic embryogenesis. Zygotic embryos from immature and mature fruits of H. bihai (L.) L. cv. Lobster Claw Two were cultured in combinations of IAA (0; 5.70, e 11.41 µm L -1 ) and 2,4-D (0; 22.62; 45.24; 67.86 e 90.48 µm L -1 ) to induce somatic embryos during 90 days of culture. Histological analyses showed that somatic embryos were formed only with zygotic embryos derived from mature fruits cultivated in the absence of growth regulators.

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Epigenetic and somaclonal divergence in Dendrocalamus farinosus for physiological augmentation and lignin degradation

Imran

Luo

et al. 2021

Biotech and App Biochem

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Epigenetic and molecular variation is a key approach for the improvement of plants. From in vitro tissue culture of Dendrocalamus farinosus; 30 plants were regenerated and after 2 years, the physiological, biochemical, and genomic studies were conducted. The results highlighted that for all phenotypic characteristics, the 30 regenerated plants were superior in comparison with the control (CK). From genetical analysis, a total of 97 bands were witnessed ranging between 212 bp and 2.2 kb. The results for OPU14 showed one additional specific band (723 bp) and one band (700 bp) were missing. The 10 plants were having genetic variability and can be termed as somaclones while the other plants have epigenetic variations. The cellulose and lignin analysis highlighted that somaclone No. 30 has the least cellulose content of 35%; while the somaclones No. 102 and No. 213 have the least 3.21% and 3.48% of lignin contents. Therefore, somaclones No. 30, No. 102, and No. 213 were selected for the histochemical localization. The lignin investigation revealed that somaclone No. 30 is greater while somaclones No. 102 and No. 213 were reduced in vascular bundles in comparison with the CK along with the high expression level of 4CL, C3H, C4H, COMT, and CCoAOMT1 genes.

show abstract

Callus Induction and Plant Regeneration from Mature Embryos of a Diverse Set of Wheat Genotypes

Cited by 69 publications

References 8 publications

Tissue culture efficiency of wheat species with different genomic formulas

Tissue culture efficiency of wheat species with different genomic formulas

Indução de embriões somáticos a partir de embriões zigóticos de Heliconia bihai (L.) L. cv. Lobster Claw Two

Epigenetic and somaclonal divergence in Dendrocalamus farinosus for physiological augmentation and lignin degradation

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