Callus Induction and Plant Regeneration in Eleven Perennial Ryegrass Cultivars

Liu, P.; Zhang, Z.-X.; Yuan, Jiangang; Xi, Jing; Du, Xuejie; Yang, Zhong

doi:10.1080/13102818.2006.10817377

Cited by 10 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, 2,4-D was found effective for callus induction and somatic embryogenesis, with a higher percentage of callus induction obtained from young shoot base explants, as compared to seed. Similarly, young shoot explants of ryegrass responded better in terms of callus induction, as reported by Liu et al (2006). These findings indicate that the response of explants depends on the requirement of exogenous plant growth regulators and specific endogenous levels (Bhaskaran and Smith 1990;Jimenez 2001).…”

Section: Discussionsupporting

confidence: 55%

Development of an embryogenic callus induction method for centipede grass (Eremochloa ophiuroides Munro) and subsequent plant regeneration

Barampuram

Chung

Lee

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

The present study demonstrates the establishment of embryogenic tissue from seeds and (seedling-derived hypocotyls) shoot base explants derived from seedlings of Eremochloa ophiuroides. The highest percentage of callus induction obtained from seed and young shoot base explants was 52.0% and 66.6% on Murashige and Skoog (MS) basal media supplemented with 9.0 μM and 18.1 µM 2,4-dichlorophenoxyacetic acid (2,4-D), respectively. The type of callus obtained from both types of explants was offwhite to yellow in color and non-friable and shiny in texture. Excised callus from the explants was subcultured onto fresh media of the same recipe for further proliferation. After 10-12 d of subculture, a yellow, globular, friable embryogenic callus was obtained from the initial callus. The highest percentage of embryogenic calli obtained at 40.0% was observed on media containing 2.2 µM 2,4-D. The highest regeneration rate of 46.6% was observed on MS media supplemented with 0.4 μM 2,4-D and 2.2 µM benzylaminopurine (BA). Regenerated shoots were rooted in MS basal medium. Plants with well-developed roots were transferred to pots containing a soil mix and acclimatized in greenhouse conditions. Four weeks post-transfer, acclimatized plants showed 100% survival and remained healthy and green. This is the first report of a successful method for induction of somatic embryogenesis with subsequent plant regeneration in centipede grass and demonstrates the establishment of embryogenic callus and efficient plant regeneration with potential application in the development of genetic transformation systems for centipede grass.

show abstract

Section: Discussionsupporting

confidence: 55%

Development of an embryogenic callus induction method for centipede grass (Eremochloa ophiuroides Munro) and subsequent plant regeneration

Barampuram

Chung

Lee

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…Drought and salinity problem of soil are serious limiting factors affecting plant growth, also are global problem restricting agriculture production. In order to reduce water demand for plant maintenance growth and grow in dry and salty areas, breeding salt, drought and high temperature-tolerant new cultivars is an urgent need (18).…”

Section: Introductionmentioning

confidence: 99%

Effect of Auxin Type and Concentrations in Different Media on the Callus Induction and Shoot Formation of Crested Wheatgrass (Agropyron Cristatum(L.) Gaertn)

Can

Çeli̇ktaş

Hatipoğlu

2008

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

“…Spontaneous albinism is a common problem encountered during tissue culture and regeneration of perennial ryegrass without genetic transformation ( Liu et al, 2006 ). The presence of these naturally occurring albino plants could complicate the identification of gene-edited pds mutant plants.…”

Section: Resultsmentioning

confidence: 99%

Agrobacterium- and a single Cas9-sgRNA transcript system-mediated high efficiency gene editing in perennial ryegrass

et al. 2022

View full text Add to dashboard Cite

Genome editing technologies provide a powerful tool for genetic improvement of perennial ryegrass, an important forage and turfgrass species worldwide. The sole publication for gene editing in perennial ryegrass used gene-gun for plant transformation and a dual promoter based CRISPR/Cas9 system for editing. However, their editing efficiency was low (5.9% or only one gene-edited plant produced). To test the suitability of the maize Ubiquitin 1 (ZmUbi1) promoter in gene editing of perennial ryegrass, we produced ZmUbi1 promoter:RUBY transgenic plants. We observed that ZmUbi1 promoter was active in callus tissue prior to shoot regeneration, suggesting that the promoter is suitable for Cas9 and sgRNA expression in perennial ryegrass for high-efficiency production of bi-allelic mutant plants. We then used the ZmUbi1 promoter for controlling Cas9 and sgRNA expression in perennial ryegrass. A ribozyme cleavage target site between the Cas9 and sgRNA sequences allowed production of functional Cas9 mRNA and sgRNA after transcription. Using Agrobacterium for genetic transformation, we observed a 29% efficiency for editing the PHYTOENE DESATURASE gene in perennial ryegrass. DNA sequencing analyses revealed that most pds plants contained bi-allelic mutations. These results demonstrate that the expression of a single Cas9 and sgRNA transcript unit controlled by the ZmUbi1 promoter provides a highly efficient system for production of bi-allelic mutants of perennial ryegrass and should also be applicable in other related grass species.

show abstract

Callus Induction and Plant Regeneration in Eleven Perennial Ryegrass Cultivars

Cited by 10 publications

References 29 publications

Development of an embryogenic callus induction method for centipede grass (Eremochloa ophiuroides Munro) and subsequent plant regeneration

Development of an embryogenic callus induction method for centipede grass (Eremochloa ophiuroides Munro) and subsequent plant regeneration

Effect of Auxin Type and Concentrations in Different Media on the Callus Induction and Shoot Formation of Crested Wheatgrass (Agropyron Cristatum(L.) Gaertn)

Agrobacterium- and a single Cas9-sgRNA transcript system-mediated high efficiency gene editing in perennial ryegrass

Contact Info

Product

Resources

About