Callus Induction and Plant Regeneration from Barley Mature Embryos (<i>Hordem Vulgare</i>L.)

Abumhadi, N.; Kamenarova, Kunka; Todorovska, Elena; Dimov, G.; Trifonova, A.; Gecheff, K.; Atanassov, A.

doi:10.1080/13102818.2005.10817224

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…While different regeneration protocols are available (Harwood, 2014; Ismagul et al, 2014) and were tested, regeneration rates did not vary significantly, and as regeneration in Golden Promise was acceptable (17%) we did not pursue further testing. Thus, as has been observed previously, barley transformant regeneration is strongly genotype‐dependent (Abumhadi et al, 2005; Al‐Nahdi, 2005; Han et al, 2011; Rostami et al, 2013). Despite the difficulties encountered, we successfully generated 22 T 0 plants from cultivar Golden Promise.…”

Section: Discussionsupporting

confidence: 66%

CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley

Karunarathne

Han

Zhang

et al. 2022

JIPB

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Nitrogen is a major determinant of grain yield and quality. As excessive use of nitrogen fertilizer leads to environmental pollution and high production costs, improving nitrogen use efficiency (NUE) is fundamental for a sustainable agriculture. Here, we dissected the role of the barley abnormal cytokinin response1 repressor 1 (HvARE1) gene, a candidate for involvement in NUE previously identified in a genome-wide association study, through natural variation analysis and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated protein 9 (Cas9)-mediated gene editing. HvARE1 was predominantly expressed in leaves and shoots, with very low expression in roots under low nitrogen conditions. Agrobacterium-mediated genetic transformation of immature embryos (cv. Golden Promise) with single guide RNAs targeting HvARE1 generated 22 T 0 plants, from which four T 1 lines harbored missense and/or frameshift mutations based on genotyping. Mutant are1 lines exhibited an increase in plant height, tiller number, grain protein content, and yield. Moreover, we observed a 1.5-to 2.8-fold increase in total chlorophyll content in the flag leaf at the grain filling stage. Delayed senescence by 10-14 d was also observed in mutant lines. Barley are1 mutants had high nitrogen content in shoots under low nitrogen conditions. These findings demonstrate the potential of ARE1 in NUE improvement in barley.

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Section: Discussionsupporting

confidence: 66%

CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley

Karunarathne

Han

Zhang

et al. 2022

JIPB

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“…Different explants such as immature embryos (Chang et al 2003), immature inflorescence (Havrlentova et al 2001), coleoptile (Sahrawat and Chand 2004), mature embryo (Abumhadi et al 2005;He and Jia 2008) and seedling explants (Sharma et al 2004) have been used for callus induction and plant regeneration in barley. In common with other cereals, immature embryos are the most commonly used explants for plant regeneration (Kachhwaha and Kothari 1996;Bregitzer et al 2000) and transformation studies (Wan and Lemaux 1994;Tingay et al 1997), but it has a very narrow developmental stage for tissue culture response, the selection of that precise stage is time consuming and labour intensive.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of regeneration potential of mature embryo derived callus in Indian cultivars of barley (Hordeum vulgare L.)

Yadav

Kothari

Kachhwaha

2011

J. Plant Biochem. Biotechnol.

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A protocol for plant regeneration in Indian cultivars of barley (Hordeum vulgare L.) has been developed using mature embryo culture. The influence of various auxins 2,4-D (2,4-dichlorophenoxyacetic acid), Dicamba (3,6-dichloro-o-anisic acid) and Picloram (4-amino-3,5,6-trichloropicolinic acid) on the callus induction and subsequent plant regeneration revealed highest percent of callus induction form cultivar (cv) BL 2 on MSB 5 medium (MS salts + B 5 vitamins) supplemented with 6 mg l −1 Picloram, but maximum number of shoot buds (6-13) were regenerated on MSB 5 medium containing 0.5 mg l −1 Picloram. Regenerated shoots were rooted on half-strength MSB 5 medium. Plantlets were successfully transferred to soil and grown to maturity in greenhouse. The effect of copper sulphate revealed significant improvement in callus induction and plant regeneration when the concentration of CuSO 4 was increased to 3 μM (30 times higher than normal MS medium) for cv BL 2. Regeneration potential differed for different cultivars of barley used, with highest for cv BL 2 and lowest for cv BH 924. We conclude that the Indian barley genotypes exhibit plant regeneration from mature embryo cultures. The protocol has potential application in barley improvement through genetic engineering.

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“…However, most studies and reviews have shown that immature embryos are the best explant source for tissue culture (Yang et al, 2015;Deng et al, Frontiers in Genetics frontiersin.org 2020; Shimizu-Sato et al, 2020). The reason for this difference may be related to the physiological state and differentiation degree of explants of different species (Abumhadi et al, 2005;Da Silva et al, 2015). In addition, ease of handling, a lack of seasonal limitations, and availability in bulk quantities are essential factors for selecting mature seed embryos for tissue culture (Yin et al, 2011;Ren et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Establishment of an efficient regeneration and Agrobacterium transformation system in mature embryos of calla lily (Zantedeschia spp.)

et al. 2022

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Calla lily (Zantedeschia spp.) have great aesthetic value due to their spathe-like appearance and richness of coloration. However, embryonic callus regeneration is absent from its current regeneration mechanism. As a result, constructing an adequate and stable genetic transformation system is hampered, severely hindering breeding efforts. In this research, the callus induction effectiveness of calla lily seed embryos of various maturities was evaluated. The findings indicated that mature seed embryos were more suitable for in vitro regeneration. Using orthogonal design experiments, the primary elements influencing in vitro regeneration, such as plant growth regulators, genotypes, and nanoscale materials, which was emergent uses for in vitro regeneration, were investigated. The findings indicated that MS supplemented with 6-BA 2 mg/L and NAA 0.1 mg/L was the optimal medium for callus induction (CIM); the germination medium (GM) was MS supplemented with 6-BA 2 mg/L NAA 0.2 mg/L and 1 mg/L CNTs, and the rooting medium (RM) was MS supplemented with 6-BA 2 mg/L NAA 0.7 mg/L and 2 mg/L CNTs. This allowed us to verify, in principle, that the Agrobacterium tumefaciens-mediated genetic transformation system operates under optimal circumstances using the GUS reporter gene. Here, we developed a seed embryo-based genetic transformation regeneration system, which set the stage for future attempts to create new calla lily varieties.

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Callus Induction and Plant Regeneration from Barley Mature Embryos (Hordem VulgareL.)

Cited by 8 publications

References 29 publications

CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley

CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley

Evaluation of regeneration potential of mature embryo derived callus in Indian cultivars of barley (Hordeum vulgare L.)

Establishment of an efficient regeneration and Agrobacterium transformation system in mature embryos of calla lily (Zantedeschia spp.)

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