Callus Induction and Plant Regeneration from Leaf Explants of Apple (Pyrus malus L.) cv. Golden Delicious

Kumar, Ravi; Joshi, Charu; Nailwal, Tapan Kumar

doi:10.20546/ijcmas.2016.502.057

Cited by 7 publications

(3 citation statements)

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“…Plant propagation in vitro by tissue culture can be employed as an alternative to providing rootstock seeds. Some of the advantages of tissue culture techniques are being able to obtain plant material in large and uniform quantities only from tissue or even from plant cells, the seeds produced are free of plant destructive organisms, and the growth of seeds that are not affected by environmental conditions such as seasons [4].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies related to apple tissue culture with various variations of PGR concentrations have been carried out, including studies by [2], [4], and [5]. Based on this research, it is known that 6benzylaminopurine (BAP) and indole-3-butyric acid (IBA) are hormones that can provide a good response to explant growth.…”

Section: Introductionmentioning

confidence: 99%

“…The use of explant types also influences the success of tissue culture techniques. The selection of leaves as a source of explants has previously been carried out in a study [4]. Whereas culture with petiole explants has never been done before.…”

Section: Introductionmentioning

confidence: 99%

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Morphogenesis Response of Leaf and Petiole Explant of Rootstock Apple (Malus sylvestris Mill.) to Auxin and Cytokinin

Nafika,

Munawarti

2024

Jurnal Biotropika

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Rootstock apple is a type of plant used in grafting propagation. Research related to the response of morphogenesis in this plant has not been widely carried out. The aim of this research was to study the effect of auxin and cytokinin ratio on the morphogenesis response of leaf and petioles explants of rootstock apple and to study variations in the ratio of auxin and cytokinin with the best response. The study used a 2-factorial Completely Randomized Design (CRD) with variations in the types of explants and combinations of plants growth regulators (PGR) in the culture medium. There were 12 treatments with five repetitions. Explants in the form of young apple leaf (third leaf) and petiole rootstock that had been sterilized and cut with a size of 5x5 mm for the leaf and 5 mm long for the petiole were inoculated into MS medium with cytokinin in the form of BAP (1 ppm) combined with auxin, IBA (0, 0.1, 0.2, 0.3, 0.4, 0.5 ppm). Each culture bottle contains five explants. The results showed that the average leaf explant was capable of producing 34% callus. Meanwhile, petiole explants produced 4% callus. Callus began to form on leaf and petiole explants on the 26th and 42nd days after inoculation respectively. Most calluses have a compact texture with variations in green, yellowish white, and brownish green. The PGR combination with the highest percentage of callus formation in leaf explants was found in the BAP 1 ppm + IBA 0.4 ppm treatment, while the petiole was in the BAP 1 ppm + IBA 0.3 ppm treatment

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%