Callus induction and haploid plant regeneration from baby primrose (Primula forbesii Franch.) anther culture

Jia, Yin; Zhang, Qixiang; Wang, Shiqin; Liu, Qinglin; Sun, Lina

doi:10.1016/j.scienta.2014.07.018

Cited by 26 publications

(13 citation statements)

References 35 publications

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“…Reportedly, root or shoot explants of Arabidopsis culture in callus-inducing medium (CIM) added with cytokinin and auxin induced callus from pericycle cells adjacent to the xylem poles. Additionally, shoot and root regeneration seemed to partly resemble the lateral root meristem formation (Jia et al 2014;Ji et al 2015). Interestingly, the root or shoot calli are not unorganized cells mass, as they were organized structures similar to the primordia of lateral roots (Jia et al 2014).…”

Section: Effect Of Various Explants On Callus Frequencymentioning

confidence: 98%

“…Additionally, shoot and root regeneration seemed to partly resemble the lateral root meristem formation (Jia et al 2014;Ji et al 2015). Interestingly, the root or shoot calli are not unorganized cells mass, as they were organized structures similar to the primordia of lateral roots (Jia et al 2014). Furthermore, the transcriptome analysis showed that the gene expression profile of these calli types is similar to the root meristems (Atta et al 2009).…”

Section: Effect Of Various Explants On Callus Frequencymentioning

confidence: 98%

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Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Abiri

Mahmood

Shaharuddin

et al. 2017

Int. J. Environ. Sci. Technol.

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Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogenesis materials was assessed in three phases, namely callogenesis, proliferation and regeneration. Initially, rice seeds were subjected under various auxin treatments. Secondly, the effect of different concentrations of 2,4-D on callus induction was evaluated. In the next step, the efficiency of different explants was identified. Subsequently, the effects of different auxins, cytokinins, L-proline, casein hydrolysate and potassium metasilicate concentrations on the callus proliferation and regeneration were considered. For the callogenesis phase, 2 mg L -1 of 2,4-D and roots were chosen as the best auxin and explant. In the callus proliferation stage, the highest efficiency was observed at week eight in the MS media supplemented with 2 mg

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Section: Effect Of Various Explants On Callus Frequencymentioning

confidence: 98%

Section: Effect Of Various Explants On Callus Frequencymentioning

confidence: 98%

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Abiri

Mahmood

Shaharuddin

et al. 2017

Int. J. Environ. Sci. Technol.

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“…DH production from anther culture of primula accelerate cultivar development in this crop. A protocol for successful callus induction and plant regeneration from Primula forbesii Franch anthers was described (Jia et al, 2014). They reported highest callus induction ratio (2.4%) on MS supplemented with 1.0 mg/l BAP + 0.5 mg/l 2,4-D and highest induction and proliferation of indefinite buds (55.2%) on MS supplemented with 0.2 mg/l BAP + 0.01 mg/l NAA.…”

Section: Baby Primrosementioning

confidence: 99%

Haploid and Double Hapoids in Ornamentals – A Review

eshwari¹,

Kumari²,

Aswath³

2018

Int.J.Curr.Microbiol.App.Sci

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“…In vitro propagation is considered to be a complimentary conservation tool (Saransan et al, 2006), and has successfully been used to protect many species of the genus Primula, including rare and endemic species (P. scotica, P. heterochroma, P. cuneifolia var. hakusanensis) (Benson et al, 2000;Sharaf et al, 2011;Shimada et al, 1997) as well as those with decorative and/or medicinal (P. veris, P. vulgaris, P. pubescens, P. forbesii) properties (Hayta et al 2016;Jędrzejczyk et al, 2018;Jia et al, 2014;Morozowska & Wesołowska, 2004;Okršlar et al, 2007;Schween & Schwenkel, 2002Takihira et al, 2007). Depending on the propagation objective, different explant types and cultivation methods were used.…”

Section: Introductionmentioning

confidence: 99%

Application of Horticultural and Tissue Culture Methods for Ex Situ Conservation of Endangered Primula farinosa L.

et al. 2020

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Our study aimed at active conservation of the last location of Primula farinosa, an endangered species in Poland, and assessed reproduction by seeds and plant propagation on sterile media in tissue culture conditions. We identified gibberellic acid (GA3) as the key factor stimulating germination of P. farinosa seeds. Growing juvenile plants under controlled temperature of 18/16 °C day/night yielded good quality plant material without mycorrhization. In tissue culture, the most favorable medium for shoot propagation was MS supplemented with the lowest tested concentration of indole-3-butyric acid (IBA; 0.05 mg dm−3) and 6-benzyl-aminopurine (BAP; 0.1 mg dm−3). The rooting ability of shoots was high and comparable for all auxins used. 2C DNA content of seed-derived and micropropagated plants did not indicate any change in the ploidy level during in vitro cultivation. Plants derived from seeds and tissue cultures were compared in a 2-year study. Of all the characteristics compared, only the number of flowers per inflorescence was lower for micropropagated plants when compared with the seed-origin plants in the first year of observation. The difference was of transient nature and was not observed in the second year of the study. Effective protocols for in vivo and in vitro propagation of P. farinosa were developed, which can be used in practical species protection.

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Callus induction and haploid plant regeneration from baby primrose (Primula forbesii Franch.) anther culture

Cited by 26 publications

References 35 publications

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Haploid and Double Hapoids in Ornamentals – A Review

Application of Horticultural and Tissue Culture Methods for Ex Situ Conservation of Endangered Primula farinosa L.

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