Callus induction and frond regeneration in Spirodela polyrhiza

Abstract

Wang Y. (2016): Callus induction and frond regeneration in Spirodela polyrhiza. Czech J. Genet. Plant Breed., 52: 114-119.Spirodela polyrhiza belongs to the family Lemnaceae (duckweed), which is a group of small aquatic plants offering an attractive plant expression system for the production of recombinant protein. No frond regeneration protocol has been established in this species yet. An efficient protocol for plant regeneration through organogenesis has been developed in Spirodela polyrhiza for the first time. Calli were successfully induced from 92% of explants on Murashige and Skoog (MS) medium with 10 μM naphthaleneacetic acid, 2 μM thidiazuron, 1μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 3% sucrose. MS medium containing 1% (m/v) sorbitol and 1 µM 2,4-D supported long lasting growth (at least 5 months) of 98% of calli. Plants regenerated from 92% of calli on Schenk and Hildebrandt (SH) medium with 10 μM zeatin and 1% (m/v) sucrose. The protocol for frond regeneration could be a good basis for transgenic engineering of S. polyrhiza.

Efficient genetic transformation and CRISPR/Cas9‐mediated genome editing in Lemna aequinoctialis

“…Second, an optimized callus induction protocol can be used. Recently, a study showed that optimizing the callus induction medium can significantly increase the rate of callus induction in S. polyrhiza (Wang, ). Our preliminary results also showed that using the optimized medium reported from Wang () can result in 100% callus induction among all 16 tested S. polyrhiza genotypes.…”

“…Recently, a study showed that optimizing the callus induction medium can significantly increase the rate of callus induction in S. polyrhiza (Wang, ). Our preliminary results also showed that using the optimized medium reported from Wang () can result in 100% callus induction among all 16 tested S. polyrhiza genotypes. It seems plausible that the optimization of the callus induction medium can be used to increase the callus induction efficiency also in other Lemna species.…”

Agrobacterium-Mediated Genetic Transformation of Taiwanese Isolates of Lemna aequinoctialis

“…Previous studies have implemented callus induction on species within Lemnoideae and have revealed that both the type and concentration of PGRs can significantly enhance the callus induction rate [ 25 , 30 , 31 ]. A crucial role is played by 2,4-D in callus induction in many plant species [ 32 , 33 , 34 ].…”

Estabelecimento de protocolo de cultura de tecidos e transformação genética para três genótipos de >i<Lemnoideae>/i<

“…A indução de calos também se mostrou possível a partir da combinação de mais de dois reguladores, como no caso da indução em Spirodela polyrhiza (Wang, 2016). O trabalho, utilizou ANA, TDZ e 2,4 D. Yang et al (2018b) apontaram em seu trabalho que a indução de calos em S. polyrhiza ocorre de maneira mais eficiente quando a concentração de auxina for igual ou superior a de citocinina.…”

“…O primeiro relato de regeneração de folhas de S. polyrhiza foi descrito por Wang (2016) As idades dos calos testados também interferiram nos resultados de eficiência de regeneração. O genótipo L. minor 8627 apresentou regeneração em todas as idades de calos testadas, e maior número de plantas (88%) a partir de calos no início de R2 -com cerca de 120 a 130 dias desde a inoculação, e no fim do subcultivo R2 -com cerca de 140 a 150 dias desde a inoculação.…”