Callus growth and plant regeneration in diverse cultivars of cucumber (Cucumis sativus L.)

Kim, Sang‐Gu; Chang, Joeng-Rahn; Cheol, Hyeon; Lee, Kwang‐Woong

doi:10.1007/bf00043109

Cited by 41 publications

(28 citation statements)

References 14 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The maximum amount of embryogenic callus was obtained on MS medium supplemented with 3.0 mg L -1 of NAA. Similar kind of somatic embryogenic studies and shoot regeneration induction was reported in cucumber leaves (Usman et al 2011;Kim et al 1988;Ali et al 1991). Then the small piece of primary globular embryogenic callus was subcultured on MS medium supplemented with 2.5 mg L -1 BAP ?…”

Section: Effect Of Naa Alone or In Combination With Bap On Induction supporting

confidence: 69%

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

Ramakrishna

Shasthree

2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

We report an efficient somatic embryogenesis and plant regeneration system using leaf cultures of Citrullus colocynthis (L.) and assessed the effect of plant growth regulators on the regeneration process. Initially leaf explants were cultured on Murashige and Skoog medium supplemented with different concentrations of auxins viz., 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid, gibberellic acid alone and along with combination of 6-benzylaminopurine. The different forms of calli such as compact, white friable, creamy friable, brownish nodular, green globular and green calli were induced from the leaf explants on MS medium containing different concentrations of auxins and gibberellins. Subsequently initial callus was subcultured at 1.5 mg L -1 BAP ? 1.0 mg L -1 2,4-D which resulted in 25 % somatic embryos from 85 % nodular embryogenic nodular callus that is highest percentage. Similarly the lowest percentage of somatic embryos was recorded at 2.5 mg L -1 BAP ? 0.5 mg L -1 NAA from 55 % embryogenic globular callus i.e., 16 %. High frequency of embryo development takes place at intermittent light when compared with continuous light in the individual subcultures. The cotyledonary embryos were developed into complete platelets on MS medium. In vitro regenerated plantlets were washed to remove the traces of agar and then transferred to sterile vermiculite and sand (2:1) containing pot.

show abstract

Section: Effect Of Naa Alone or In Combination With Bap On Induction supporting

confidence: 69%

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

Ramakrishna

Shasthree

2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…Differences in explant response among cultivars of the same species has been observed in cucumber, Cucumis sativus L . (Kim et al ., 1988), melon (Gray et al ., 1993 ;Niedz et al ., 1989), potato (Cardi et al ., 1993 ;M'Ribu & Veilleux, 1990), tomato 'Collective diameter of all 3 flexuous anthers per flower . Y Leaf length by width ratio ; obtained by dividing the leaf length by its width .…”

Section: Methodsmentioning

confidence: 99%

Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro

1996

View full text Add to dashboard Cite

Adventitious shoots were obtained from the diploid watermelon [Citrullus lanatus (Thunb .) Matsum . & Nakai] cultivars Dixielee, Jubilee II, Mickylee, Minilee, and Royal Sweet by culturing excised cotyledons on shoot regeneration medium for six weeks. Tetraploid and diploid regenerants were identified by counting the number of chloroplasts per guard cell pair from leaves of regenerated plants . Cross fertilization of putative tetraploids with diploid pollinators and the production of triploid seed confirmed the efficacy of this approach . The mean number of chloroplasts for tetraploid regenerants was 19 .1 whereas diploids averaged 11 .2 . These values were similar to tetraploid and diploid plants from seed . Ovary diameter, petal, and anther diameter of male flowers, and leaf length by width ratio were also good indicators of plant ploidy . Progeny obtained from self-fertile tetraploids of 'Mickylee' were crossed with various diploid pollinators to produce triploid hybrid seed . All triploid plants from tissue culture-derived tetraploids produced fruit comparable in quality to fruit produced by currently-available triploid hybrids, demonstrating that in vitro tetraploid induction can be used to produce high quality tetraploid plants for use in triploid hybrid seed production .

show abstract

“…Variety of explants have been used as starting material for plant regeneration from cucumber, such as nodal segments (Ahmad & Anis, 2005), shoot tip (Vasudevan et al, 2001;Jafar & Nuray, 2007), cotyledons (Trulson & Shahin, 1986;Kim et al, 1988;Chee, 1990), hypocotyl explants (Rajasekaran et al, 1983;Ziv & Gadasi, 1986;Selvaraj et al, 2006), primary leaves (Chee & Tricoli, 1988;Malepszy & Nadolska-Orezyk, 1989;Seo et al, 2000), and petioles (Punja et al, 1990). Regeneration of cucumber is also possible through protoplast, leaf-derived callus (Abu-Romman et al, 2013), somatic embryogenesis (Chee, 1990), and cell suspension culture (Chee & Tricoli, 1988;Raharjo & Punja, 1992;Lou & Kako, 1994;Vengadesan et al, 2005;Ugandhar et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

Abu-Romman¹,

Al-Hadid²,

Arabiyyat³

2015

JAS

View full text Add to dashboard Cite

In vitro shoot multiplication of cucumber (Cucumis sativus) was examined from the nodal explants of 10-day-old aseptic plantlets using Murashige and Skoog (MS) media supplemented with different concentration (0, 0.5, 1, 2, 3 mg L -1 ) of cytokinins (6-Benzylaminopurine-BAP, Kinetin-Kn, Thidiazuron-TDZ, and Zeatin). Nodal explants of cucumber showed shoot induction and multiplication in response to all cytokinins tried. MS medium containing Kn was the most effective for inducing shoots from nodal explants of cucumber. The maximum rate of regeneration (83%), the highest number of obtained shoots (7.93 shoots/explant) and the longest shoots (3.61 cm) were obtained on MS medium fortified with 1 mg L -1 Kn. The lowest culture responses were recorded for media supplemented with either BAP or Zeatin. In conclusion, using Kn is strongly recommended than using Zeatin, TDZ and BAP to obtain the highest percentage of regeneration, the highest number of shoot/explant, and the highest shoot length for nodal explants of cucumber.

show abstract

Callus growth and plant regeneration in diverse cultivars of cucumber (Cucumis sativus L.)

Cited by 41 publications

References 14 publications

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro

Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

Contact Info

Product

Resources

About