Callus Formation from Protoplasts Derived from Cell Suspension Cultures of Vicia faba L.

Röper, W.

doi:10.1016/s0044-328x(81)80063-3

Cited by 14 publications

(2 citation statements)

References 11 publications

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“…After the protoplasts were isolated from leaves and shoots [150,151] and suspension cells [152], the regeneration of faba bean plant from protoplasts was reported [153]. Somatic embryogenesis in callus and suspension cultures derived from immature cotyledons of faba bean [154], a protocol in which plantlets derived from somatic embryos could be grown to maturity [155] and an improved protocol combined with the Agrobacterium tumefaciens-mediated gene transfer [156] were subsequently been achieved.…”

Section: In Vitro Regenerationmentioning

confidence: 99%

Impact of Molecular Technologies on Faba Bean (Vicia faba L.) Breeding Strategies

Gnanasambandam¹,

Paull

Torres

et al. 2012

Agronomy

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Faba bean (Vicia faba L.) is a major food and feed legume because of the high nutritional value of its seeds. The main objectives of faba bean breeding are to improve yield, disease resistance, abiotic stress tolerance, seed quality and other agronomic traits. The partial cross-pollinated nature of faba bean introduces both challenges and opportunities for population development and breeding. Breeding methods that are applicable to self-pollinated crops or open-pollinated crops are not highly suitable for faba bean. However, traditional breeding methods such as recurrent mass selection have been established in faba bean and used successfully in breeding for resistance to diseases. Molecular breeding strategies that integrate the latest innovations in genetics and genomics with traditional breeding strategies have many potential applications for future faba bean cultivar development. Hence, considerable efforts have been undertaken in identifying molecular markers, enriching genetic and genomic resources using high-throughput sequencing technologies and improving genetic transformation techniques in faba bean. However, the impact of research on practical faba bean breeding and cultivar release to farmers has been limited due to disconnects between research and breeding objectives and the high costs of research and implementation. The situation with faba bean is similar to other small crops and highlights the need for coordinated, collaborative research programs that interact closely with commercially focused breeding programs to ensure that technologies are implemented effectively

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Section: In Vitro Regenerationmentioning

confidence: 99%

Impact of Molecular Technologies on Faba Bean (Vicia faba L.) Breeding Strategies

Gnanasambandam¹,

Paull

Torres

et al. 2012

Agronomy

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show abstract

“…There have been two attempts to regenerate plants using protoplast culture. In one experiment, 20% of the protoplasts were capable of cell division and 4% formed a colony or callus (Röper, 1981). In another, 40% of the protoplasts divided and regeneration frequency was 5-10%, but only one cultivar out of ten tested was able to regenerate plants and the entire procedure took 14 months (Tegeder et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Indirect Organogenesis of Faba Bean (Vicia Faba L. Minor)

Skrzypek¹,

Czyczyło-Mysza²,

Marcińska³

2012

Acta Biologica Cracoviensia Series Botanica

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Using four Polish Vicia faba L. minor cultivars (Bronto, Dino, Tibo, Nadwiślański) we obtained callus from epicotyl fragments collected from 7-and 14-day-old seedlings and from cotyledonary nodes of immature seeds. Callus induction efficiency varied from 81% to 97% depending on the origin of the explant. Shoots regenerated only from the cotyledonary nodes of all tested cultivars. On average, 50% of the explants grown on MS medium containing 1.0 mg dm -3 NAA, 0.5 mg dm -3 BAP, 0.25 mg dm -3 GA 3 , 1.0 g dm -3 casein hydrolysate, 750 mg dminositol, 3% sucrose and 0.4% agar were able to regenerate shoots. The number of calluses regenerating shoots was highest from explants collected from fruiting nodes 6 to 9. Decapitation of donor plants increased the percentage of calluses regenerating shoots. On half-strength MS medium with 2 mg dm -3 NAA and on 1/2 MS alone, 11% of the shoots rooted; on 1/2 MS with 1 g dm -3 AC, 8.0% rooted. The regenerants were transferred to Perlite with Hoagland medium and acclimated. Ten percent of the regenerated plants survived the acclimation process, flowered and produced seeds.K Ke ey y w wo or rd ds s: : Callus, immature seed, organogenesis, in vitro culture, Vicia faba L. minor.

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Tissue Culture Techniques for Virus Elimination and Germplasm Preservation

Kartha

1984

Plant Breeding Reviews

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Callus Formation from Protoplasts Derived from Cell Suspension Cultures of Vicia faba L.

Cited by 14 publications

References 11 publications

Impact of Molecular Technologies on Faba Bean (Vicia faba L.) Breeding Strategies

Impact of Molecular Technologies on Faba Bean (Vicia faba L.) Breeding Strategies

Indirect Organogenesis of Faba Bean (Vicia Faba L. Minor)

Tissue Culture Techniques for Virus Elimination and Germplasm Preservation

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