Callus formation and plant regeneration in various Lilium species and cultivars

Mori, Satoshi; Adachi, Yukiko; Horimoto, Sakae; Suzuki, Sakae; Nakano, Masaru

doi:10.1079/ivp2005707

Cited by 34 publications

(28 citation statements)

References 13 publications

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“…The highest bulblet regeneration was obtained on N6 medium containing 4 mg/L picloram. The promotory effect of picloram and dicamba on callus formation and bulblet regeneration has also been reported previously for many bulbous plant species (Xu et al, 2000;Mori et al, 2005, Khosravi et al, 2007Ault and Siqueira, 2008). Khosravi et al (2007) investigated the effect of various concentrations of picloram on direct somatic embryogenesis in Lilium and indicated that MS medium supplemented with 2 mg/L picloram was the most effective treatment for induction of embryogenesis.…”

Section: Discussionsupporting

confidence: 74%

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In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Uzun¹,

Parmaksız²,

Uranbey³

et al. 2014

Turk J Biol

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An efficient in vitro bulblet production procedure from immature zygotic embryos of endemic and endangered Muscari muscarimi Medik. was described in the current study. Zygotic embryos were first isolated from immature seeds and cultured on different nutrient media compositions supplemented with various combinations of α-naphthalene acetic acid (NAA), picloram, dicamba, 6-benzylaminopurine (BAP), and thidiazuron (TDZ). The best bulblet regeneration (59 bulblets per explant) was achieved in Murashige and Skoog (MS) medium containing 4 mg/L BAP and 0.5 mg/L NAA after 1 year of culture initiation. Regenerated bulblets were then transferred into MS medium without plant growth regulators for rooting. Bulblets produced well-developed root systems and increased their size on this medium after 2 months. All rooted bulblets were successfully transplanted into a potting mixture and acclimatized to ambient conditions.

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Section: Discussionsupporting

confidence: 74%

“…Bulb scales are the most common explants used for in vitro propagation of geophytes (Mirici et al, 2005;Mori et al, 2005). However, the use of bulbs as starting material is often associated with heavy bacterial or fungal contamination (Langens-Gerrits et al, 1998;Ziv and Liliens-Kipnis, 2000;Karagüzel et al, 2012;Liu et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Uzun¹,

Parmaksız²,

Uranbey³

et al. 2014

Turk J Biol

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show abstract

“…As such, TCL cultures would also appear to be suitable as a valuable target tissue for Agrobacterium transformation. In some Lilium species and cultivars, genetic transformations have already been performed successfully (Watad et al 1998;Hoshi et al 2004;Mori et al 2005;Kim et al 2007). The procedure described here is efficient and can be applied in programs aimed at conserving this valuable genetic resource.…”

Section: Somatic Embryo Germination and Plant Acclimatizationmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration of Lilium ledebourii (Baker) Boiss., an endangered species

Bakhshaie

Babalar

Mirmasoumi

et al. 2010

Plant Cell Tiss Organ Cult

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A somatic embryogenesis (SE) protocol was established for the regeneration of Lilium ledebourii (Baker) Boiss. whole plants using new vegetative bulblet microscales and transverse thin cell layers (tTCLs) of young bulblet roots as the explant sources. Bulblets were induced from bulb scale explants cultured for at least 3 months in the dark on Murashige and Skoog (MS) medium containing 3% sucrose, 0.8% agar, and different concentrations of a-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and thidiazuron. Embryo-like structures were obtained from tTCL explants of 3-month-old bulblets (excised from bulb scale explants) following culture on solid MS medium containing 3% sucrose and various concentrations of NAA and BA for 3 months in the dark. Both the explant source and the type of plant growth regulators affected the differentiation of somatic embryos. The highest percentage (65.55%) of embryogenesis was obtained from bulblet microscale tTCLs cultured on solid MS medium containing 0.54 lM NAA and 0.44 lM BA. Plants with normal shoots and roots were obtained following a 3-month culture of embryos on growth regulatorfree MS medium at 25 ± 1°C under a 16/8-h light/dark photoperiod (light intensity 40 lmol m -2 s -1 , cool-white fluorescent light). The plants were successfully acclimatized in the growth chamber.

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“…Mori (11) reported that scales displayed the greatest ability to form callus in 23 Lilium genotypes, followed by leaves. There have been only a few reports on petioles used as explants for callus induction.…”

Section: Rooting and Transplantationmentioning

confidence: 99%

“…Bulblets regeneration has been achieved from a vast array of explants ranging from vegetative organs to reproductive organs (1,9,10,14,15,16,17). Callus formation and plant regeneration from induced callus were observed in 33 Lilium genotypes (11). Most successful reports of in vitro regeneration of Lilium have focused on L. longiflorum genotypes and other popular Lilium species (10,13,14,15,16,17).…”

Section: Introductionmentioning

confidence: 99%

Callus Induction and Plant Regeneration fromIn VitroCultured Leaves, Petioles and Scales ofLilium Leucanthum(Baker) Baker

Tang

Liu

Gituru

et al. 2010

Biotechnology & Biotechnological Equipment

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Callus formation and plant regeneration in various Lilium species and cultivars

Cited by 34 publications

References 13 publications

In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Somatic embryogenesis and plant regeneration of Lilium ledebourii (Baker) Boiss., an endangered species

Callus Induction and Plant Regeneration fromIn VitroCultured Leaves, Petioles and Scales ofLilium Leucanthum(Baker) Baker

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