Callus extract of Ipomoea mauritiana show analgesic and antihyperglycemic activity in Swiss albino mice

Abstract

Callus can be a viable alternative to obtain important phytochemicals and analyze crude extract for pharmacological activities rather than going the cumbersome way of collecting and destroying possibly endangered plants. In this study, callus was produced using nodal explant of Ipomoea mauritiana, and methanol extract of dried and powdered callus was evaluated for its analgesic and antihyperglycemic potential. The extract, when administered to Swiss albino mice at doses of 50, 100, 200 and 400 mg per kg significantly reduced the number of writhings in mice produced by intraperitoneal administration of acetic acid by 23.3, 33.3, 43.3, and 53.3%, respectively. A standard analgesic drug, aspirin, at doses of 200 and 400 mg per kg, reduced the number of writhings by 40.0 and 46.7%, respectively. In oral glucose tolerance tests conducted with glucose-loaded mice, the extract at doses of 50, 100, 200 and 400 mg per kg significantly reduced blood glucose levels by 35.1, 42.5, 53.6, and 58.8%. In comparison, a standard antihyperglycemic drug, glibenclamide, at a dose of 10 mg per kg reduced blood glucose levels by 60.7%. Our study indicates that methanol extract of callus of I. mauritiana can be used to alleviate pain and high blood sugar levels.

Medicinal uses, pharmacology, and phytochemistry of Convolvulaceae plants with central nervous system efficacies: A systematic review

“…Due to the hallucinogenic content of ergoline alkaloids in the seeds, a lot of Ipomoea species were used by ancient people to attain a state of mind suitable for divination during religious ceremonies and magical rituals in Central America and Africa. For instance, the seeds of I. violacea, known as tlitliltzin in the Aztec language, are Islam, Ahmed, et al (2015) Ipomoea obscura Usiboniseleni (Z)…”

Comparative assessment of in vitro antidiabetic activity of Tinospora cordifolia stem, leaves and its callus extracts

“…If calluses can be seen to give the desired pharmacological effect or have the requisite phytochemical(s), they can serve the purpose of various plant parts including underground parts, and such calluses can be obtained within a relatively small space in the laboratory or industry and so negating the uprooting of plants and as a result endangering them. (Islam et al,2015) The herb Tinospora cordifolia (Menispermaceae) is commonly known as Guduchi in India. A variety of chemical constituents such as alkaloids, diterpenoid lactones, steroids, glycosides aliphatic compounds, polysaccharides have been reported from different parts of Tinospora cordifolia.…”