Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases

Cimino, Cecilia; Cavalli, Sandra Vairo; Spina, Francisco; Natalucci, Claudia L.; Priolo, Nora

doi:10.2225/vol9-issue3-fulltext-14

Cited by 15 publications

(9 citation statements)

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“…A solution could be the production of S. marianum biomass from in vitro cultures. Cimino et al (2006) have developed the composition of a culture medium for callus production of S. marianum.…”

Section: Human Nutritionmentioning

confidence: 99%

Silybum marianum: non-medical exploitation of the species

2015

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Silybum marianum (milk thistle) is currently utilised as a medicinal plant which provides raw material for the production of silymarin. Silymarin is composed of a group of flavonolignans that are well known and studied for their medicinal properties. However, the biomass yield potential and the chemical composition of its biomass suggest opportunities for much broader utilisation of S. marianum. This paper reviews the available literature about S. marianum biomass productivity and composition and about properties of products and byproducts of silymarin extraction. Alternative uses of whole plant biomass include fodder, bioenergy production and phytoremediation. Byproducts of silymarin extraction such as oil and flour have possible applications in food, feed and cosmetics. Moreover, the review explores potential alternative applications of silymarin. We conclude that a pivotal issue for further utilisation of S. marianum is the development of improved cultivars suited for the different possible utilisations.

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Section: Human Nutritionmentioning

confidence: 99%

Silybum marianum: non-medical exploitation of the species

2015

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“…5). Callus cultures of S. marianum were also reported as potential sources of milk clotting peptidases (Cimino et al 2006). Positive linear correlation between antioxidant activity and total phenolic content for alcoholic extracts of S. marianum was reported by Tawaha et al (2007).…”

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confidence: 93%

“…1a). Previously, Cimino et al (2006) induced callus from cotyledons of S. marianum incubated on B5 medium (Gamborg et al 1968) supplemented with 0.05 mg l -1 BA and 0.5 mg l -1 of 2,4-dicholorophenoxyacetic acid (2,4-D). However, Iqbal and Srivastava (2000) induced callus from leaf, shoot apex and nodal explants of seedlings of S. marianum incubated on medium containing NAA, BA, and Zn.…”

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confidence: 99%

“…In this study, leaf explants from wild-grown plants showed high frequency of callogenesis (88%) and organogenesis (76%). However, callus production may be important for studies of indirect morphogenesis (Radice and Caso 1997) or for studies of production of secondary metabolites in medicinal plants (Meratan et al 2009;Cimino et al 2006). Frequency of organogenesis was determined after 30 days of sub-culture and the mean number of shoots per culture flask was significantly higher (25.5 ± 2.0 shoots per flask) when explants were incubated on medium containing 2.0 mg l -1 GA 3 and 1.0 mg l -1 NAA than all other PGR treatments tested (Figs 1b, 3 and 4).…”

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confidence: 99%

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Shoot regeneration and free-radical scavenging activity in Silybum marianum L.

Abbasi

Khan

Mahmood

et al. 2010

Plant Cell Tiss Organ Cult

105

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The morphogenic potential and free-radical scavenging activity of the medicinal plant, Silybum marianum L. (milk thistle) were investigated. Callus development and shoot organogenesis were induced from leaf explants of wild-grown plants incubated on media supplemented with different plant growth regulators (PGRs). The highest frequency of callus induction was observed on explants incubated on Murashige and Skoog (MS) medium supplemented with 5.0 mg l -1 6-benzyladenine (BA) after 20 days of culture. Subsequent transfer of callogenic explants onto MS medium supplemented with 2.0 mg l -1 gibberellic acid (GA 3 ) and 1.0 mg l -1 a-naphthaleneacetic acid (NAA) resulted in 25.5 ± 2.0 shoots per culture flask after 30 days following culture. Moreover, when shoots were transferred to an elongation medium, the longest shoots were observed on MS medium supplemented with 0.5 mg l -1 BA and 1.0 mg l -1 NAA, and these shoots were rooted on a PGR-free MS basal medium. Assay of antioxidant activity of in vitro and in vivo grown tissues revealed that significantly higher antioxidant activity was observed in callus than all other regenerated tissues and wild-grown plants.

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“…Some animal enzymes, like urokinase and tissue plasminogen activator are being produced from in-vitro cultivation of established cell lines (Mizrahi 1986;Avgerinos et al 1990;Zang et al 1995;Altamirano et al 2006). Some plant enzymes, like invertase (Pressi et al 2003), neutral protease (Cimino et al 2006) and acid phosphatase (Su and Arias 2003) are produced by in-vitro plant cell cultivation. Only high value enzymes for sophisticated applications are potential candidates for in-vitro production with animal and plant cell lines, because of the complexities and high costs of production at large scale (Chu and Robinson 2001;Hood 2002).…”

mentioning

confidence: 99%

Enzyme Production

Illanes

2008

Enzyme Biocatalysis

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Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases

Cited by 15 publications

References 9 publications

Silybum marianum: non-medical exploitation of the species

Silybum marianum: non-medical exploitation of the species

Shoot regeneration and free-radical scavenging activity in Silybum marianum L.

Enzyme Production

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