Intrahospital infections (IIH) generate a considerable increase in morbidity and mortality, with the consequent increase in hospitalization costs. They are considered a controllable epidemic problem, but difficult to eradicate; however, through effective programs their incidence can be significantly reduced and morbidity and costs drastically reduced, optimizing the limited allocation of resources for health. In the research, samples of indoor environment were collected from two private centers located in the city of Valencia, Venezuela, samples such as water, air, surfaces. Whose samples have been processed according to Spanish environmental regulations and even through official gazettes and the standard method for drinking water and waste water. The results yielded alarming data, data that were quantified and identified microorganisms causing hospital-acquired infections, which are easily dispersed by hospital areas, affecting susceptible patients, non-susceptible, visitors and staff in general.