Calicivirus Biology

Gutiérrez-Escolano, Ana Lorena

doi:10.1007/978-3-319-54567-7_3

Cited by 2 publications

(2 citation statements)

References 78 publications

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“…Figure 1 depicts the cell invasion mechanisms of the most common viruses used as surrogates to determine antiviral action of EOs and NPs, among them FCV, herpes virus (HSV), MNV, HAV and SARS‐CoV. Virus replication begins with their interaction mediated by specific receptors present in the host cell membrane, for example, JAM‐1 for FCV (Akiko et al, 2006; David et al, 2008; Gutierrez‐Escolano, 2017; Pesavento et al, 2010), CD300lf for MNV (Can et al, 2008; Haga et al, 2016; Taube et al, 2009, 2010; Turgay et al, 2022), ACE for SARS‐CoV‐2 (Cantuti‐Castelvetri et al, 2020; Duan et al, 2020; Mousavizadeh & Ghasemi, 2021; J. S. Yang et al, 2020), sialic acid binding for nHAV (A. Das et al, 2020), and TIM1‐like PS receptors for eHAV (A. Das, 2020). This interaction causes conformational changes in the viral particle, which favor the subsequent stages.…”

Section: Background On the Use Of Antipathogenic Substances In Packag...mentioning

confidence: 99%

Nanoparticles and essential oils with antiviral activity on packaging and surfaces: An overview of their selection and application

Márquez

Zwilling

Zambrano

et al. 2022

J Surfact & Detergents

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Until recently, food packaging has been used as a barrier to unfavorable environmental and microbial conditions, but new technologies are arising aimed at upgrading the protective nature of these materials. Cases of bacterial and viral transmission through food and surfaces have shown the vulnerabilities of the food packaging distribution systems over the years, creating awareness about new methods to prevent the proliferation of pathogens. This has been highlighted by safety concerns due to the COVID‐19 pandemic. This work reviews the state‐of‐the‐art biobased technologies tailored for antiviral applications on surfaces, focusing on packaging materials. A survey and selection tool of essential oils (EOs) and nanoparticles that have been proven effective in reducing the proliferation or transmission of viruses through surfaces is presented. Additionally, the use of essential oil formulations or nanoparticle‐functionalized biobased materials that can be deployed to prevent virus transmission through food produce and surfaces is reviewed, including environmental and safety concerns around the use of metal nanoparticles and EOs in packaging materials. Finally, an assessment of the available technologies, challenges, opportunities and the potential role of biobased antiviral surfaces in future viral outbreaks is presented.

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Section: Background On the Use Of Antipathogenic Substances In Packag...mentioning

confidence: 99%

Nanoparticles and essential oils with antiviral activity on packaging and surfaces: An overview of their selection and application

Márquez

Zwilling

Zambrano

et al. 2022

J Surfact & Detergents

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“…Trascripción in vitro de la región 5´ UTR de SCMV. Para la transcripción in vitro se siguió la metodología descrita previamente [19]. La región 5´UTR de SCMV fue amplifi cada por PCR incluyendo la secuencia del promotor T7 en el oligo 5´.…”

Section: Introductionunclassified

Exploración de la interacción entre la región 5´UTR del Sugarcane Mosaic Virus (SCMV) y proteínas del hospedero maíz

Chaves-Bedoya

2017

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Resumen Antecedentes: El virus del mosaico de la caña de azúcar, SCMV (Potyviridae) es un miembro de la gran familia de virus de ARN de cadena positiva, sin una estructura CAP en su extremo 5´no traducido (5`UTR), pero con una proteína viral unida al genoma (VPg) y una cola poli A en su extremo 3´UTR. Se ha sugerido que proteínas del hospedero hacen un puente entre las regiones no traducidas virales 5´y 3´ para potenciar la traducción viral. Dado que las regiones no traducidas presentes en los genomas virales contienen elementos involucrados en la regulación de su ciclo replicativo, es importante analizar la interacción entre estas regiones y las proteínas virales o del hospedero para inferir su función. Objetivo: Determinar si existen proteínas del hospedero maíz que pudieran estar interactuando con la región 5´UTR de SCMV. Metodología: La región 5´UTR de SCMV se amplificó por PCR incluyendo la secuencia del promotor T7 en la secuencia del oligo 5´ UTR y se generaron sus transcritos marcados radiactivamente. Los transcritos marcados fueron entrecruzados con extractos proteicos de hojas de maíz sanas e infectadas. Resultados: Los resultados sugieren la presencia de una proteína putativa de interacción del hospedante maíz de aproximadamente 53kDa, con la región 5´UTR de SCMV. Conclusión: Los ensayos de entrecruzamiento, especificidad y estabilidad de los complejos ARN-proteína sugieren que en el hospedante maíz existe al menos una proteína que interactúa con la región 5´ UTR de SCMV.Palabras Clave: Complejo ARN-proteína, entrecruzamiento UV, fracción proteica, Potyvirus, VPg.Abstract Background: Sugarcane mosaic virus is a member of the great family of positive sense RNA viruses, without a CAP structure in its 5´UTR end, but with a viral protein attached to the genome (VPg) and a poli A tail in its 3´UTR end. It has been suggested that some host proteins make a bridge between the untranslated 5´and 3´ regions (UTRs) to enhance the canonical and/or non-canonical virus translation. Since the UTR regions present in the viral genomes have some elements involved in the regulation of their replicative cycle, it is important to analyze the interaction that may occur between these regions and the viral or host proteins to infer regarding its function. Objective: To identify proteins of the host maize that could be interacting with the 5´UTR region of SCMV. Methodology: 5´UTR region of SCMV was amplified by PCR including the sequence of the T7 promoter in the 5´oligo sequence. The transcripts were radioactively labeled. Labeled transcripts were crosslinked with proteins extracts from healthy and infected maize leaves. Results: The results suggest the presence of a 53 kDa putative protein interacting with the 5 ´UTR region of SCMV. Conclusion: Crosslinking essays, specificity and the stability of the RNA-protein complex suggest that in maize host there is at least one protein that interacts with the 5´UTR region of SCMV.Keywords: RNA-Protein complex, UV crosslinking, protein fraction, Potyvirus, VPg

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Calicivirus Biology

Cited by 2 publications

References 78 publications

Nanoparticles and essential oils with antiviral activity on packaging and surfaces: An overview of their selection and application

Nanoparticles and essential oils with antiviral activity on packaging and surfaces: An overview of their selection and application

Exploración de la interacción entre la región 5´UTR del Sugarcane Mosaic Virus (SCMV) y proteínas del hospedero maíz

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