Calibration of light forces in optical tweezers

Felgner, Harald; Müller, Otto; Schliwa, Manfred

doi:10.1364/ao.34.000977

Cited by 161 publications

(88 citation statements)

References 12 publications

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“…8 confirm the conclusions of previous works, 10,11 according to which the trap efficiency increases with the particle diameter. Though similar in order of magnitude, our values are lower than those of Wright et al 11 and Felgner et al 10 for 2a =1 m, we find Q Ќ Х 0.037, to be compared to 0.08, 10 ͑this measurement was performed in glycerol solution͒ and 0.13. 11 Taking into account the above remarks on hydrodynamic coupling and spherical aberration, our value may be raised up to about 0.048, still below the other values.…”

Section: ͑0͒supporting

confidence: 90%

“…10,11,15,24,29,30 Conversely to the hydrodynamic coupling, the spherical aberration increases when h / a increases, causing the decrease of F Ќ for h / a Ͼ 5 ͑see Fig. 4; note that in this region F Ќ ͑0͒ Х F Ќ ͒.…”

Section: ͑0͒mentioning

confidence: 97%

“…4͒ is only apparent, being caused by the hydrodynamic coupling of the particle with the wall. In theory, this artifact can be eliminated by applying a correction factor to the measured escape force, 10,11,25 .…”

Section: A Single-beam Trapmentioning

confidence: 99%

“…In comparison to the two-beam concept, the single-beam trap has the twofold advantage of simplicity and size independence; i.e., particles with sizes ranging from less than 1 m up to about 20 m can be trapped with the same setup, without any adjustment except that of the beam power. 10,11 Simplicity stems from the fact that the trap just needs a single collimated beam, directed through a microscope objective with a very large aperture. The laser beam must be expanded to be wide enough to cover the exit pupil of the objective.…”

Section: Introductionmentioning

confidence: 99%

“…The test procedures are similar to those used in recent works about RP forces and the influence of spherical aberration in optical tweezers. 10,11,15 Long-WD threedimensional trapping in two-beam mode is demonstrated, and transverse trapping efficiencies are measured as a function of the interfoci distance ͑⌬͒, for various particle sizes. In both modes, we test the linearity of the measured trapping force versus the laser power.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Implementing both short- and long-working-distance optical trappings into a commercial microscope

Kraikivski

Pouligny

Dimova

2006

Review of Scientific Instruments

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Optical tweezers are now a widespread tool based on three-dimensional trapping by a tightly focused single laser beam. This configuration only works with large numerical aperture and short-working-distance (SWD) objectives, restricting optical manipulation to the high magnification end of the microscope nosepiece. Certain applications of optical trapping demand long-working distances (LWDs) at moderate magnification, imposing a more complex two-beam trapping configuration. In this article, we describe a complete setup that incorporates both SWD and LWD optical trapping functionalities into a single Axiovert 200M Zeiss microscope. We evaluate the performance of the setup in both trapping modes with latex particles, either fluorescent or not, of different sizes, in the 1–20μm range. We provide practical information allowing for optimal configuration of the two-beam geometry, in relation with longitudinal and lateral stabilities of the trap.

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Section: ͑0͒supporting

confidence: 90%

Section: ͑0͒mentioning

confidence: 97%

Section: A Single-beam Trapmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Implementing both short- and long-working-distance optical trappings into a commercial microscope

Kraikivski

Pouligny

Dimova

2006

Review of Scientific Instruments

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An adjustable multi‐scale single beam acoustic tweezers based on ultrahigh frequency ultrasonic transducer

Chen

Lam

Chen

et al. 2017

Biotech & Bioengineering

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This paper reports the fabrication, characterization, and microparticle manipulation capability of an adjustable multi-scale single beam acoustic tweezers (SBAT) that is capable of flexibly changing the size of "tweezers" like ordinary metal tweezers with a single-element ultrahigh frequency (UHF) ultrasonic transducer. The measured resonant frequency of the developed transducer at 526 MHz is the highest frequency of piezoelectric single crystal based ultrasonic transducers ever reported. This focused UHF ultrasonic transducer exhibits a wide bandwidth (95.5% at -10 dB) due to high attenuation of high-frequency ultrasound wave, which allows the SBAT effectively excite with a wide range of excitation frequency from 150 to 400 MHz by using the "piezoelectric actuator" model. Through controlling the excitation frequency, the wavelength of ultrasound emitted from the SBAT can be changed to selectively manipulate a single microparticle of different sizes (3-100 μm) by using only one transducer. This concept of flexibly changing "tweezers" size is firstly introduced into the study of SBAT. At the same time, it was found that this incident ultrasound wavelength play an important role in lateral trapping and manipulation for microparticle of different sizes. Biotechnol. Bioeng. 2017;114: 2637-2647. © 2017 Wiley Periodicals, Inc.

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Dynamically controlled dielectrophoresis using resonant tuning

et al. 2021

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Electrically polarizable micro-and nanoparticles and droplets can be trapped using the gradient electric field of electrodes. But the spatial profile of the resultant dielectrophoretic force is fixed once the electrode structure is defined. To change the force profile, entire complex lab-on-a-chip systems must be re-fabricated with modified electrode structures. To overcome this problem, we propose an approach for the dynamic control of the spatial profile of the dielectrophoretic force by interfacing the trap electrodes with a resistor and an inductor to form a resonant resistor-inductor-capacitor (RLC) circuit. Using a dielectrophoretically trapped water droplet suspended in silicone oil, we show that the resonator amplitude, detuning, and linewidth can be continuously varied by changing the supply voltage, supply frequency, and the circuit resistance to obtain the desired trap depth, range, and stiffness. We show that by proper tuning of the resonator, the trap range can be extended without increasing the supply voltage, thus preventing sensitive samples from exposure to high electric fields at the stable trapping position. Such unprecedented dynamic control of dielectrophoretic forces opens avenues for the tunable active manipulation of sensitive biological and biochemical specimen in droplet microfluidic devices used for single-cell and biochemical reaction analysis.

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Calibration of light forces in optical tweezers

Cited by 161 publications

References 12 publications

Implementing both short- and long-working-distance optical trappings into a commercial microscope

Implementing both short- and long-working-distance optical trappings into a commercial microscope

An adjustable multi‐scale single beam acoustic tweezers based on ultrahigh frequency ultrasonic transducer

Dynamically controlled dielectrophoresis using resonant tuning

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