Calibration by the standard addition and indicative dilution method in flame atomic absorption spectrometry

Kościelniak, Paweł; Kozak, Joanna; Wieczorek, Marcin

doi:10.1039/c1ja10024f

Cited by 13 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…148 The authors claim that this approach works better than the normal standard additions approach providing the interferences are not multiplicative. 148 The authors claim that this approach works better than the normal standard additions approach providing the interferences are not multiplicative.…”

Section: Instrumentationmentioning

confidence: 99%

“…A standard addition and indicative dilution method has been developed which consists of addition of a standard solution to a sample and then successive dilution of this until the signal is equal to that produced by the undiluted sample. 148 The authors claim that this approach works better than the normal standard additions approach providing the interferences are not multiplicative.…”

Section: Instrumentationmentioning

confidence: 99%

See 1 more Smart Citation

Atomic spectrometry update. Advances in atomic spectrometry and related techniques

Evans

Day

Palmer

et al. 2012

J. Anal. At. Spectrom.

View full text Add to dashboard Cite

Section: Instrumentationmentioning

confidence: 99%

Section: Instrumentationmentioning

confidence: 99%

Atomic spectrometry update. Advances in atomic spectrometry and related techniques

Evans

Day

Palmer

et al. 2012

J. Anal. At. Spectrom.

View full text Add to dashboard Cite

“…Denitions, terms and classications of different calibration approaches have been presented in several papers [1][2][3][4] and some novel calibration strategies aimed at overcoming the problem of analytical inaccuracy have been developed. 2,[5][6][7][8][9][10] However, each calibration procedure allows for only one estimate of analyte concentration in a sample.…”

Section: Introductionmentioning

confidence: 99%

Novel multicommuted flow manifold dedicated to the integrated calibration method

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…In spite of the above-mentioned drawbacks, at present, the use of internal standard calibration using SIL-IS is the best option available to ensure the removal of matrix effects from LC-MS data. In practice, SIL-IS are generally very expensive and they are often not commercially The standard addition method, taught through analytical chemistry text books as a calibration method to be used when matrix effects cannot be eliminated by physical means, has been widely used in spectrophotometric analysis, especially in atomic spectroscopy (240)(241)(242)(243).…”

Section: Introductionmentioning

confidence: 99%

Development of an assay for vitamin D in biological samples

Kassim¹,

Sofiah²

View full text Add to dashboard Cite

The role of vitamin D in bone health has been well established. Recent studies have provided a greater focus on the ways in which vitamin D and its metabolites have additional functions in the body that subsequently influence conditions such as common cancers, autoimmune diseases, cardiovascular diseases, depression and schizophrenia. Vitamin D is known to have more than 50 metabolites. The major circulating form in man is 25(OH)D3; having a long half-life, this compound is considered the principal biomarker therefore the best indicator of vitamin D status. Measuring 25(OH)D3 will, however, only provide an incomplete picture of vitamin D status that may or may not correlate with functions in disease. The 1,25dihydroxyvitamin D metabolites (1,25(OH)2D3 and 1,25(OH)2D2) are the most active forms that are responsible for bone health; evidence has now accumulated indicating its role in brain development. The 24,25-dihydroxyvitamin D metabolites (24,25(OH)2D3 and 24,25(OH)2D2) are responsible for bone fracture repair. The biological roles of vitamin D-sulfate compounds (D3-S, D2-S, 25(OH)D3-S and 25(OH)D2-S) are unclear to date, probably due to the lack of sufficiently sensitive assay methods to study them. It has been suggested that sulfated compounds are the storage forms of the non-conjugated metabolites,and may have similar potencies. Considering the above information, and given the diverse biological roles of vitamin D, it is important to accurately quantify as many forms as possible. Although widely used, immunoassay-based methods are not suitable for this purpose because of their inability to differentiate between some of these compounds. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) has been the method of choice in recent years because of its high selectivity and sensitivity. This thesis describes the development and validation of an LC-MS/MS method for the quantitative analysis of 12 vitamin D compounds, specifically: D2, D3, 25(OH)D2, 25(OH)D3, 24,25(OH)2D2, 24,25(OH)2D3, 1,25(OH)2D2, 1,25(OH)2D3, D3-S, D2-S, 25(OH)D3-S and 25(OH)D2-S in biological fluids. During the initial method development, samples of human plasma and serum were compared. It was found that serum yielded higher concentrations of vitamin D compounds. Protein precipitation was compared with saponification method for extraction of vitamin D from biological fluids and found to be more effective. Vitamin D metabolites are known to have low ionisation efficiencies therefore produce weak signals in iii MS detector. Pre-column derivatisation procedure using 4-phenyl-1,2,4-triazoline-3,5-dione, (PTAD) was attempted to enhance the ionisation efficiencies of 12 vitamin D compounds. However, this method failed to detect the sulfated compounds, except for traces of 25(OH)D3-S and the derivatisation procedure proved unsuitable for sulfated compounds. The principal analytical challenge was that of extracting and chromatographing vitamin D compounds with very different lipophilicities in a single analysis procedure. The aim was therefore to o...

show abstract

Calibration by the standard addition and indicative dilution method in flame atomic absorption spectrometry

Cited by 13 publications

References 21 publications

Atomic spectrometry update. Advances in atomic spectrometry and related techniques

Atomic spectrometry update. Advances in atomic spectrometry and related techniques

Novel multicommuted flow manifold dedicated to the integrated calibration method

Development of an assay for vitamin D in biological samples

Contact Info

Product

Resources

About