Calculations for reproducible autologous skin cell-spray grafting

Esteban-Vives, Roger; Young, Matthew P.; Zhu, Toby; Beiriger, Justin; Pekor, Chris; Ziembicki, Jenny; Corcos, A; Rubin, P.; Gerlach, Jörg C.

doi:10.1016/j.burns.2016.06.013

Cited by 24 publications

(13 citation statements)

References 27 publications

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“…It involved isolating the epidermal cells from a small skin sample by enzymatic digestion, preparing a basal cell-enriched suspension and redistributing the cells by spraying or dripping to the debrided or granulated wound area to facilitate permanent wound closure or to correct the pigmentation loss. The isolated living cells were then re-distributed immediately to a larger wound surface area at 1:80–100 expansion ratio for fast wound epithelisation [ 110 ].…”

Section: Reviewmentioning

confidence: 99%

Cell therapy for severe burn wound healing

Maitz

2018

Burns &Amp; Trauma

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Cell therapy has emerged as an important component of life-saving procedures in treating burns. Over past decades, advances in stem cells and regenerative medicine have offered exciting opportunities of developing cell-based alternatives and demonstrated the potential and feasibility of various stem cells for burn wound healing. However, there are still scientific and technical issues that should be resolved to facilitate the full potential of the cellular devices. More evidence from large, randomly controlled trials is also needed to understand the clinical impact of cell therapy in burns. This article aims to provide an up-to-date review of the research development and clinical applications of cell therapies in burn wound healing and skin regeneration.

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Section: Reviewmentioning

confidence: 99%

Cell therapy for severe burn wound healing

Maitz

2018

Burns &Amp; Trauma

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“…Cell spraying, by means of aerosolization of cell suspensions in air, has been used previously to spray keratinocytes on skin wounds to accelerate healing (Kirsner et al, 2012;Esteban-Vives et al, 2016b), melanocytes to restore skin color (Iman et al, 2013), and bladder urothelial and smooth muscle cells to reconstitute segments of colon used in bladder augmentation (Hidas et al, 2015). However, there is an urgent need to develop improved methods of spraying considering large and irregular wounds and cutaneous injuries.…”

Section: Discussionmentioning

confidence: 99%

Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing

Chang

Liu

Guo³

et al. 2020

Front. Bioeng. Biotechnol.

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Severe skin wounds are often associated with large areas of damaged tissue, resulting in substantial loss of fluids containing electrolytes and proteins. The net result is a vulnerability clinically to skin infections. Therapies aiming to close these large openings are effective in reducing the complications of severe skin wounds. Recently, cell transplantation therapy showed the potential for rapid re-epithelialization of severe skin wounds. Here, we show the improved effects of cell transplantation therapy using a robust protocol of efficient expansion and delivery of epidermal cells for treatment of severe skin wounds. Human skin tissues were used to generate human epidermal organoids maintained under newly established culture conditions. The human epidermal organoids showed an improved capacity of passaging for at least 10 rounds, enabling organoids to expand to cell numbers required for clinical applications. A newly designed auto micro-atomization device (AMAD) was developed for delivery of human epidermal organoids onto the sites of severe skin wounds enhancing uniform and concentrated delivery of organoids, facilitating their engraftment and differentiation for skin reconstitution. With the optimal design and using pneumatic AMAD, both survival and functions of organoids were effectively protected during the spraying process. Cells in the sprayed human epidermal organoids participated in the regeneration of the epidermis at wound sites in a mouse model and accelerated wound healing significantly. The novel AMAD and out new protocol with enhanced effects with respect to both organoid expansion and efficient transplantation will be used for clincal treatments of complex, uneven, or large-area severe skin wounds.

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“…The question arises whether a large wound area can be covered by the harvest of cells without expansion from a small skin specimen. In an in vitro study an expansion ratio of over 1:100 was calculated for uncultured cells sprayed with a density of 10 4 cells/cm 2 for an estimated surface coverage [112] . However, there is no other literature to support the claimed expansion rate.…”

Section: Application Types Of Keratinocytes Transplantationmentioning

confidence: 99%

Advances in keratinocyte delivery in burn wound care

Horst

Chouhan

Moiemen

et al. 2018

Advanced Drug Delivery Reviews

171

129

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This review gives an updated overview on keratinocyte transplantation in burn wounds concentrating on application methods and future therapeutic cell delivery options with a special interest in hydrogels and spray devices for cell delivery.To achieve faster re-epithelialisation of burn wounds, the original autologous keratinocyte culture and transplantation technique was introduced over 3 decades ago. Application types of keratinocytes transplantation have improved from cell sheets to single-cell solutions delivered with a spray system. However, further enhancement of cell culture, cell viability and function in vivo, cell carrier and cell delivery systems remain themes of interest.Hydrogels such as chitosan, alginate, fibrin and collagen are frequently used in burn wound care and have advantageous characteristics as cell carriers.Future approaches of keratinocyte transplantation involve spray devices, but optimisation of application technique and carrier type is necessary.

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Calculations for reproducible autologous skin cell-spray grafting

Cited by 24 publications

References 27 publications

Cell therapy for severe burn wound healing

Cell therapy for severe burn wound healing

Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing

Advances in keratinocyte delivery in burn wound care

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