Calculation of the Results of Microbiological Assays

Wood, Eric C.

doi:10.1038/155632a0

Cited by 24 publications

(7 citation statements)

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“…3 are W = 387e0O3ISD and W = 347eO°°O7D, respectively. The intercepts (387-and 347-mg uteri) are close, allowing the LT sample to be assayed as a dilution of the CT sample (11). Thus, the potency of the LT sample is 0.00507/0.0318 or 16% that of the CT sample.…”

Section: Resultsmentioning

confidence: 99%

Characterization of uterine growth response to cholera toxin in hamsters and test of heat-labile enterotoxin from Escherichia coli

Alleva¹,

Lamanna²

1984

J Clin Microbiol

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Cholera toxin (CT) and the heat-labile enterotoxin from Escherichia coli, when injected intraperitoneally into cycling hamsters but not rats or mice, induced a massive uterine growth similar to that normally induced by the implanting blastocyst during pregnancy. CT and heat-labile enterotoxin are the only known agents that have this action in any species. Uterine weight reached a maximal sixfold increase 48 h after injection of CT. Concurrent injection of estrogen, progesterone, and CT increased the maximal response to eightfold and eliminated differences in the response to CT injected on different days of the 4-day hamster estrous cycle. The dose response for CT, heat-labile enterotoxin, and CT plus estrogen plus progesterone was most linear (r > 0.93) when the logarithm of uterine weight was plotted against the dose of toxin. The hamster uterine weight response can serve as a simple, highly precise, and highly specific bioassay for CT and heat-labile enterotoxin.

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Section: Resultsmentioning

confidence: 99%

Characterization of uterine growth response to cholera toxin in hamsters and test of heat-labile enterotoxin from Escherichia coli

Alleva¹,

Lamanna²

1984

J Clin Microbiol

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“…After incubation for 72 h at 38" the growth of micro-organisms was measured by titration with 0.05 M-NaOH. Results were cvaluated by the method of Wood (1945) and expressed as g/16 g N. All assays were replicated at least three times.…”

Section: I972mentioning

confidence: 99%

The use of hydroxyproline analyses to predict the nutritional value of the protein in different animal tissues

Dvořák¹

1972

Br J Nutr

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1. The amounts of available cystine and tyrosine in the protein of different animal tissues showed a close correlation with the level of hydroxyproline, and may be estimated from hydroxyproline values by regression equations.2. Estimates of ‘chemical score’ have been calculated from the content of hydroxyproline determined in a series of samples for which net protein utilization (NPU) for rats had also been determined. Chemical scores calculated as percentages of the total ‘essential+semiessential’ amino acid content of each material correlated closely with NPU, whereas scores calculated as percentages of total amino acids did not. ‘Methionine+cystine’ were calculated to be first limiting amino acids in every sample.

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“…Eine ausfuhrliche Veroffentlichung oon I<. SIGLE [37]). Nach REIF [38] ist die MeBgenauigkeit der mikrobiologischen Methode bei Verwendung von Leuconostoc a EAA-Index = Essential-Amino-Acid-Index; deutsch : EAS-Index = Essentielle Aminosauren-Index.…”

Section: Bisherige Arbeitenunclassified

Die Kartoffel als hochwertige Eiweißquelle nach Ergebnissen von Ernährungsversuchen und von Bausteinanalysen

Schuphan¹,

Weinmann²

1959

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Calculation of the Results of Microbiological Assays

Cited by 24 publications

References 0 publications

Characterization of uterine growth response to cholera toxin in hamsters and test of heat-labile enterotoxin from Escherichia coli

Characterization of uterine growth response to cholera toxin in hamsters and test of heat-labile enterotoxin from Escherichia coli

The use of hydroxyproline analyses to predict the nutritional value of the protein in different animal tissues

Die Kartoffel als hochwertige Eiweißquelle nach Ergebnissen von Ernährungsversuchen und von Bausteinanalysen

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