Calculation of the Paternity Index for STR with tri-allelic patterns in paternity testing

Yang, Qinrui; Li, Jinliang; Shao, Chengchen; Zhou, Yuxiang; Hui, Li; Li, Chengtao; Tang, Qiqun; Xie, Jianhui

doi:10.1016/j.forsciint.2021.110832

Cited by 5 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Triallelic genotypes are rare but are expected to be encountered at all traditional regions used in forensic DNA analysis 6 . This has implications for paternity index calculations, with new methods being formulated according to the generation and genetic transmission of tri-allelic pattern 18 . All the triallelic genotypes observed in this study except for 1 were type 2 suggesting chromosomal duplication or aneuploidy where multiple alleles with peaks of equal height are produced 6 .…”

Section: Discussionmentioning

confidence: 99%

Analysis of data and common mutations encountered during routine parentage testing in Zimbabwe

Thelingwani,

Jonhera,

Masimirembwa

2024

Sci Rep

View full text Add to dashboard Cite

We analyzed parentage data collected over a ten-year period in a Zimbabwean DNA testing laboratory. Parentage case types, prevalence, exclusion data, mutations rates and observed genotyping irregularities were analyzed. We report analysis results from 1303 cases. DNA extraction and STR typing was conducted using standard commercial kits. Paternity was the most requested test (87.37%) followed by the indirect biological kinship tests (7.01%). Duo paternity (motherless) was the most common paternity test for both regular and court cases. We observed 367 paternity exclusions from 1135 cases, giving an overall paternity exclusion rate of 32.33%. Maternity had the lowest exclusion rate (8.33%), with criminal cases having the highest paternity (61.11%) and maternity (33.33%) exclusion rates. The number of mismatched STR loci ranged from 2–12 for duo cases and 4–18 for the trio cases. FGA, D2S1338, D18S51 and D2S441 were the most informative markers for exclusion. We detected 30 mutations out of 837 cases with an estimated paternal and maternal mutation rate of 0.0021 and 0.0011 respectively. Triallelic patterns were only observed at the TPOX locus with allele 10 and 11 being the extra alleles transmitted. Our report provides forensic parameters which can improve parentage and forensic analysis in Zimbabwe.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of data and common mutations encountered during routine parentage testing in Zimbabwe

Thelingwani,

Jonhera,

Masimirembwa

2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Short tandem repeats (STRs), also known as microsatellites, are repeat sequences of 2–6 bp nucleotides and common genetic variants in the human genome ( Edwards et al, 1992 ). STRs are also viewed as gold-standard genetic markers for forensic identity testing and parentage analysis owing to their high diversities and wide distributions in the human genome ( Cheng et al, 2021 ; Vullo et al, 2021 ; Yang et al, 2021 ; Zhang et al, 2021 ). In 1998, Budowle et al (1998 ) proposed a combined DNA index system (CODIS) that included 13 core STRs.…”

Section: Introductionmentioning

confidence: 99%

Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation

et al. 2022

View full text Add to dashboard Cite

Short tandem repeats (STRs) are the most frequently used genetic markers in forensic genetics due to their high genetic diversities and abundant distributions in the human genome. Currently, the combined DNA index system is commonly incorporated into various commercial kits for forensic research. Some novel STRs that are different from the combined DNA index system were not only used to assess complex paternity cases but also could provide more genetic information and higher forensic efficiency in combination with those commonly used STRs. In this study, we validated forensic performance of a novel multiplex amplification STR panel to evaluate its sensitivity, species specificity, forensic application values, and so on. Obtained results revealed that the kit showed high sensitivity, and the complete allelic profile could be observed at 0.125 ng DNA sample. In addition, the kit possessed high species specificity, good tolerance to common inhibitors, and accurate genotyping ability. More importantly, STRs out of the kit displayed high discrimination power and probability of exclusion. To sum up, the novel kit presented in this study can be viewed as a promising tool for forensic human identification and complex paternity analysis.

show abstract

Statistical Interpretation of Forensic DNA Evidence

Dash,

Elkins,

Al-Snan

2023

Advancements in Forensic DNA Analysis

View full text Add to dashboard Cite

Calculation of the Paternity Index for STR with tri-allelic patterns in paternity testing

Cited by 5 publications

References 19 publications

Analysis of data and common mutations encountered during routine parentage testing in Zimbabwe

Analysis of data and common mutations encountered during routine parentage testing in Zimbabwe

Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation

Statistical Interpretation of Forensic DNA Evidence

Contact Info

Product

Resources

About